Wilms Tumour LOH-1p16q (Affymetrix OncoScan Plus Assay) Test: Booking, Price, and Results
About Wilms Tumour LOH-1p16q (Affymetrix OncoScan Plus Assay) Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | Wilms Tumour LOH 1p/16q Test, Nephroblastoma LOH Analysis, Wilms Tumour Chromosomal Microarray, WT LOH Testing |
| Sample Type | FFPE Tissue Block (formalin-fixed, paraffin-embedded tumour tissue) |
| Fasting Required | No – not applicable; sample is obtained during surgery or biopsy |
| Report Time | 8 days |
| Recommended For | Paediatric patients diagnosed with Wilms tumour (nephroblastoma), typically children under 5 years old |
| Price | Starting at ₹84,000 |
What is a Wilms Tumour LOH-1p16q (Affymetrix OncoScan Plus Assay) Test?
The Wilms Tumour LOH-1p16q test is a specialised molecular analysis performed on tumour tissue from children diagnosed with Wilms tumour, the most common kidney cancer in paediatric patients. It examines specific chromosomal regions to identify genetic changes that influence how aggressively the disease may behave. Also known as the Wilms Tumour Chromosomal Microarray or Nephroblastoma LOH Analysis, the test uses the Affymetrix OncoScan Plus Assay platform, which analyses DNA extracted from preserved tumour tissue.
What Does a Wilms Tumour LOH-1p16q (Affymetrix OncoScan Plus Assay) Test Measure?
The Wilms Tumour LOH-1p16q test procedure uses a SNP (single-nucleotide polymorphism) microarray to detect chromosomal changes in tumour cells. LOH, or loss of heterozygosity, means one copy of a specific gene region has been lost in tumour cells.
The following parameters are evaluated in this test:
| Parameter | What it Detects |
|---|---|
| LOH at Chromosome 1p | Loss of one copy of a region on the short arm of chromosome 1 |
| LOH at Chromosome 16q | Loss of one copy of a region on the long arm of chromosome 16 |
| Combined LOH 1p/16q | Presence of both chromosomal losses together |
| 1q Gain | An extra copy of a region on chromosome 1, an emerging risk marker |
| Copy Number Variations (CNVs) | Structural changes, including deletions, duplications, and unbalanced translocations |
| Ploidy and Mosaicism | Abnormal chromosome numbers and mixed cell populations within the tumour |
Why is a Wilms Tumour LOH-1p16q (Affymetrix OncoScan Plus Assay) Test Done?
The Wilms Tumour LOH-1p16q test helps oncologists understand the biological behaviour of the tumour so that treatment can be matched to the risk level.
Common Symptoms That May Require This Test
A child may be referred for this test after a Wilms tumour is confirmed. The following symptoms typically prompt the initial investigation:
- A firm, painless lump or swelling in the abdomen
- Abdominal or flank pain
- Blood in the urine (haematuria)
- Persistent fever without a clear cause
- Loss of appetite or unexplained weight loss
- Fatigue or low energy
Conditions This Test Can Help Detect
Once a Wilms tumour is diagnosed, this test is used to identify specific genetic risk factors. It can help detect:
- Chromosomal loss at 1p and/or 16q in favourable histology Wilms tumour
- Combined LOH 1p/16q, found in approximately 6% of patients with favourable histology disease and associated with significantly higher relapse rates
- Patients at elevated risk of relapse and death who may benefit from intensified treatment
- Chromosomal imbalances such as CNVs, ploidy changes, and mosaicism within the tumour
How to Prepare and What to Expect
The Wilms Tumour LOH-1p16q test procedure does not require any preparation from the patient or family in the traditional sense, as the sample is collected during a surgical or biopsy procedure.
Do You Need to Fast?
No fasting is required. This test uses tumour tissue, not blood or urine. Sample collection takes place in a hospital setting during nephrectomy (surgical removal of the kidney) or a tumour biopsy.
Practical Tips Before Your Test
The treating surgical and oncology team will manage sample collection. Families can help by keeping the following in mind:
- Bring a detailed clinical history, including the child's symptoms, previous imaging results, and prior treatment records, as this is required for the test
- Ensure the surgical team is informed that an FFPE tissue block is needed for this molecular analysis
- The tissue sample must contain at least 40% tumour content for reliable results
- Proper formalin fixation and paraffin embedding of the tissue is essential; discuss this with the treating hospital team
Step-by-Step Procedure
The sample collection and testing process follows these steps:
- The child undergoes nephrectomy or tumour biopsy at the treating hospital; lymph node sampling is also recommended at this stage.
- The surgically collected tumour tissue is preserved in formalin and embedded in a paraffin block (FFPE), or sent as fresh or frozen tissue.
- The FFPE tissue block is transported at ambient temperature (18 to 28 degrees Celsius) to the Lupin Diagnostics laboratory.
- In the laboratory, DNA is extracted from the tumour tissue; only a very small amount of genetic material (approximately 80 nanograms) is required.
- The Affymetrix OncoScan Plus Assay analyses the DNA to identify chromosomal losses, gains, and other structural changes.
- Results are reviewed and reported by a specialist, with the full report available within 8 days of sample receipt.
Factors That Can Affect Accuracy
Several factors can influence the quality and reliability of results:
- Tumour cell percentage in the submitted sample (minimum 40% required)
- DNA quality and quantity extracted from the FFPE tissue
- Proper formalin fixation and paraffin embedding at the point of collection
- Tumour heterogeneity, where different parts of the tumour may carry different genetic profiles
- Availability of matching normal tissue from the same patient for comparison
Understanding Your Wilms Tumour LOH-1p16q (Affymetrix OncoScan Plus Assay) Test Results
This is a qualitative test. Results are reported as "Detected" or "Not Detected" for each chromosomal region. A paediatric oncologist will interpret findings alongside tumour stage, histology, the child's age, and overall clinical picture.
| Parameter | Result | Interpretation |
|---|---|---|
| LOH 1p | Detected / Not Detected | Detected indicates a higher relapse risk |
| LOH 16q | Detected / Not Detected | Detected indicates a higher relapse risk |
| Combined LOH 1p/16q | Both Detected / Not Detected | Both detected indicate the highest risk category |
| 1q Gain | Detected / Not Detected | Detected is an emerging adverse prognostic marker |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Sample quality and tumour biology can affect how results are read.
Tumours with low tumour cell content or poor DNA quality from FFPE tissue may yield inconclusive results, requiring repeat sampling or an alternative approach. Tumour heterogeneity means different sections of the same tumour can show different genetic profiles, so the location of the biopsy may influence findings.
How to Maintain Healthy Levels
After receiving results, the paediatric oncology team will guide the family on next steps. General recommendations include:
- Attend all scheduled follow-up appointments with the paediatric oncologist
- Maintain regular imaging reviews as recommended to monitor for recurrence
- Follow the prescribed treatment plan closely and report any new symptoms to the treating team promptly
Lupin Diagnostics Wilms Tumour LOH-1p16q (Affymetrix OncoScan Plus Assay) Test Price
The Wilms Tumour LOH-1p16q test cost at Lupin Diagnostics starts at ₹84,000. This test requires a visit to a Lupin Diagnostics centre or submission of the FFPE tissue block through the treating hospital; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 84000 |
| CHENNAI | 84000 |
| HYDERABAD | 84000 |
| KOLKATA | 84000 |
| NAVI MUMBAI | 84000 |
| PUNE | 84000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book the Wilms Tumour LOH-1p16q test online or through the Lupin Diagnostics network:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time, or arrange submission of the FFPE tissue block through your treating hospital.
- Receive your report via email or WhatsApp within 8 days of sample receipt.
Frequently Asked Questions
Wilms tumour, also called nephroblastoma, is the most common kidney cancer in children and the fourth most common paediatric cancer overall. It is typically diagnosed in children younger than five years old, though it can occur up to age 15.
This test identifies specific chromosomal losses in tumour tissue that are linked to a significantly higher risk of relapse and death. Knowing whether these markers are present allows oncologists to adjust treatment intensity to match the child's actual risk level.
The test requires tumour tissue collected during nephrectomy (surgical removal of the kidney) or a tumour biopsy. The tissue is preserved as an FFPE block, and DNA is then extracted for microarray analysis in the laboratory.
No. The sample must be obtained during a surgical procedure at a hospital. The FFPE tissue block is then submitted to the Lupin Diagnostics laboratory for processing. Home collection is not available for this test.
The full report is available within 8 days of the laboratory receiving the tissue sample. This includes DNA extraction, microarray analysis using the Affymetrix OncoScan Plus Assay, and specialist interpretation.
Detection of LOH at chromosome 1p or 16q, or both, places the patient in a higher risk group. Intensified chemotherapy regimens have been shown to help overcome these adverse risk factors, and the treating oncologist will discuss the appropriate treatment plan based on results.
The test is primarily performed on paediatric patients, as Wilms tumour is largely a childhood cancer. About 95% of cases are diagnosed before age 10. Rare adult cases do occur, and the test may occasionally be requested for older patients on clinical judgment.
Wilms Tumour LOH-1p16q (Affymetrix OncoScan Plus Assay) Test: Booking, Price, and Results
