Trisomy - 4-FISH Test: Booking, Price, and Results
About Trisomy - 4-FISH Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | Chromosome 4 FISH, CEP4 FISH, Trisomy 4 by FISH, FISH for Chromosome 4 |
| Sample Type | Bone marrow aspirate or peripheral blood |
| Fasting Required | No |
| Report Time | 8 days |
| Recommended For | All ages; primarily patients with suspected haematological malignancies |
| Price | Starting at ₹4,400 |
What Is a Trisomy - 4-FISH Test?
The Trisomy - 4-FISH test uses a technique called Fluorescence In Situ Hybridisation (FISH) to count the number of copies of chromosome 4 in a patient's cells. Normally, each cell contains two copies of every chromosome. This test checks whether an extra copy of chromosome 4 is present, a finding known as trisomy 4. Also referred to as CEP4 FISH, the test is typically ordered by a haematologist or oncologist when a blood cancer is suspected. The sample required is either bone marrow aspirate or peripheral blood.
What Does a Trisomy - 4-FISH Test Measure?
This test examines the number of copies of chromosome 4 present in cells from the sample. Normally, each cell contains two copies of every chromosome. The table below explains what the test looks for.
| Parameter | What It Detects |
|---|---|
| Chromosome 4 copy number | Whether cells carry the normal two copies or an abnormal extra copy |
| Signal pattern per cell | The percentage of cells showing an extra chromosome 4 signal |
| Numerical chromosomal change | Presence of trisomy 4 (three copies instead of two) in the sampled cells |
The laboratory typically counts signal patterns across 200 cells and reports the percentage showing abnormality.
Why Is a Trisomy - 4-FISH Test Done?
Doctors order this test when a patient shows signs that may point to a blood cancer or related blood disorder. The test helps confirm a diagnosis, guide treatment decisions and track disease over time.
Common Symptoms That May Require This Test
A haematologist may recommend the Trisomy-4-FISH test if a patient presents with any of the following:
- Persistent, unexplained fatigue
- Frequent or recurring infections
- Unusual bleeding or bruising without clear cause
- Bone pain
- Enlarged lymph nodes
- Unexplained weight loss
- Pallor (pale skin)
Conditions This Test Can Help Detect
The test is used to identify chromosomal changes linked to several blood-related conditions, including:
- Acute myeloid leukaemia (AML), where trisomy 4 may appear as the sole abnormality or alongside other chromosomal changes
- T-cell acute lymphoblastic leukaemia (T-cell ALL), where trisomy 4 may appear as a sole chromosomal change
- B-cell acute lymphoblastic leukaemia (B-cell ALL), particularly in children, where combined trisomies of chromosomes 4 and 10 are associated with a favourable outcome
- Mixed phenotype acute leukaemia (MPAL)
- Myelodysplastic syndromes (MDS)
Trisomy - 4-FISH Test for Chronic Disease Monitoring
The Trisomy-4-FISH test is a valuable tool for monitoring patients already diagnosed with leukaemia. It can track how well treatment is working and detect any signs of disease returning after therapy. Oncologists may repeat the test at key treatment milestones, during follow-up visits and when minimal residual disease (very small amounts of remaining cancer cells) needs to be assessed. Regular monitoring with this test supports informed decisions about ongoing therapy.
How to Prepare and What to Expect
This test may involve a bone marrow aspiration procedure, which requires some specific preparation. Here is what you need to know before your appointment.
Do You Need to Fast?
No fasting is required for the Trisomy-4-FISH test procedure. However, if your doctor advises sedation or any form of anaesthesia for the bone marrow collection, you may need to follow separate dietary instructions provided by your clinical team.
Practical Tips Before Your Test
Keep the following in mind when preparing for your appointment:
- Bring a detailed clinical history, including your symptoms, previous test results and family history. This is required for the test.
- Inform your doctor about all medications you are currently taking, especially blood thinners or anticoagulants.
- Discuss any concerns about the bone marrow aspiration procedure with your haematologist before the appointment.
- Disclose any allergies, particularly to local anaesthetics, before the procedure begins.
- Arrange for someone to accompany you if sedation is planned.
Step-by-Step Procedure
This test requires a bone marrow aspirate or a peripheral blood sample, collected by a trained specialist. Here is what to expect:
Peripheral Blood Sample Collection:
- A trained phlebotomist cleans the skin over a vein, usually in the arm.
- A needle is used to collect approximately 3 ml of blood into a lavender-top EDTA tube.
- The needle is removed, and a small bandage or cotton swab is applied to the site.
- The sample is labelled and stored at 2 to 8 degrees Celsius for transport.
Bone Marrow Collection:
- A doctor performs the bone marrow collection, usually from the hip bone, under local anaesthesia or mild sedation.
- The skin over the collection site (usually the hip bone) is cleaned and numbed with a local anaesthetic.
- A small amount of bone marrow, approximately 3 ml, is drawn using a thin needle. This procedure is called bone marrow aspiration.
- The sample is placed in a green-capped sodium heparin tube and stored at 2 to 8 degrees Celsius for safe transport.
- The sample is sent to the cytogenetics laboratory, where fluorescent probes are applied to the cells on a glass slide.
- A specialist uses a fluorescence microscope to count chromosome 4 signals in the cells and prepares your report.
Factors That Can Affect Accuracy
Several factors may influence the reliability of your Trisomy - 4-FISH test results:
- Poor sample quality or low cell viability at the time of collection
- Delayed transport to the laboratory beyond the acceptable window
- Use of an incorrect anticoagulant container
- Prior chemotherapy or radiation therapy, which may alter chromosomal findings
- An insufficient number of cells in the collected sample
Understanding Your Trisomy - 4-FISH Test Results
Results from this test should always be reviewed with a qualified haematologist or oncologist. The table below shows how signal patterns in the cells are interpreted.
| Finding | Signals per Cell | Interpretation |
|---|---|---|
| Normal (disomy) | 2 signals | No evidence of trisomy 4 |
| Abnormal (trisomy 4) | 3 or more signals | Extra copy of chromosome 4 detected |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain circumstances can affect how results are read or how much information the test provides:
- The FISH technique targets only chromosome 4. It does not detect structural chromosomal abnormalities, mosaicism (when only some cells carry an abnormality) or changes on other chromosomes. For this reason, doctors usually recommend running FISH alongside routine cytogenetic analysis.
- False-positive or false-negative results are possible. Clinical context and additional investigations are always needed to confirm findings.
- Blood contamination of the bone marrow sample and a very low proportion of abnormal cells in the marrow may also affect detection sensitivity.
How to Maintain Healthy Levels
Chromosomal abnormalities like trisomy 4 are not caused by lifestyle factors and cannot be prevented through diet or exercise. If you have been diagnosed with a related blood condition, the following general guidance may support your overall wellbeing:
- Attend all follow-up appointments and repeat monitoring tests as advised by your oncologist.
- Maintain a balanced diet and adequate rest to support your body during treatment.
- Avoid known environmental carcinogens and minimise unnecessary exposure to radiation.
- Communicate openly with your healthcare team about any new or changing symptoms.
Lupin Diagnostics Trisomy – 4-FISH Test Price
The Trisomy - 4-FISH test cost at Lupin Diagnostics starts at ₹4,400. This test requires a visit to a Lupin Diagnostics centre, as the sample collection must be performed in a clinical setting. Home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 4400 |
| CHENNAI | 4400 |
| HYDERABAD | 4400 |
| KOLKATA | 4400 |
| NAVI MUMBAI | 4400 |
| PUNE | 4400 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps for Trisomy - 4-FISH test online booking:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The Trisomy - 4-FISH test detects whether cells carry an extra copy of chromosome 4. It is primarily used in the diagnosis of blood cancers such as acute myeloid leukaemia (AML) and acute lymphoblastic leukaemia (ALL). It also helps doctors plan treatment and monitor disease response.
The test can be performed on a bone marrow aspirate or a peripheral blood sample. Bone marrow aspiration is typically preferred, as it provides a more direct picture of the cells involved in haematological malignancies.
Bone marrow aspiration can cause some discomfort. A local anaesthetic is used to numb the area before the procedure, and mild sedation may be offered if you are anxious. The procedure is performed by a trained specialist to ensure your safety and comfort.
It means that three copies of chromosome 4 were found in the cells examined, rather than the normal two. In children with B-cell acute lymphoblastic leukaemia (ALL), combined trisomies of chromosomes 4 and 10 are associated with a positive outlook. In adult acute myeloid leukaemia (AML), the significance depends on the patient's age and other chromosomal findings. Your haematologist will explain what the result means for your specific situation.
At Lupin Diagnostics, the report is delivered within 8 days of sample collection. Turnaround time may vary depending on sample quality and laboratory processing.
Yes. Clinical history is required when submitting the sample. Bring your medical records, previous test reports and a summary of your symptoms to your appointment. This information helps the cytogenetics team interpret your results accurately.
No. This test is not a prenatal screening test. It is a specialised oncology test used to diagnose and monitor blood cancers. Prenatal FISH tests typically target other chromosomes to screen for conditions such as Down syndrome.
No. The Trisomy - 4-FISH test requires bone marrow aspiration or a peripheral blood sample, which must be performed by a trained healthcare professional at a clinical facility. Home collection is not available for this test.
Trisomy - 4-FISH Test: Booking, Price, and Results
