Trisomy-10-FISH Test: Booking, Price, and Results
About Trisomy-10-FISH Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | Chromosome 10 Enumeration by FISH, FISH for Trisomy 10, CEP10 FISH, Chromosome 10 Aneuploidy FISH, Trisomy 10 by Chromosome Enumeration Probe |
| Sample Type | Bone marrow and peripheral blood |
| Fasting Required | No fasting required |
| Report Time | 8 days |
| Recommended For | All ages; primarily used in haematological malignancy workup, particularly paediatric B-cell acute lymphoblastic leukaemia |
| Price | Starting at ₹4,400 |
What Is a Trisomy-10-FISH Test?
The Trisomy-10-FISH test uses Fluorescence In Situ Hybridisation (FISH), a laboratory method that attaches fluorescent tags to specific chromosomes so they can be counted under a specialised microscope. This test looks at chromosome 10 specifically, checking whether cells carry the normal two copies or an abnormal third copy (trisomy). The test is also known as Chromosome 10 Enumeration by FISH or CEP10 FISH. It is typically ordered by haematologists and oncologists when a blood cancer is suspected or already diagnosed, and it requires a bone marrow aspirate and a peripheral blood sample.
What Does a Trisomy-10-FISH Test Measure?
The Trisomy-10-FISH test procedure analyses the number of copies of chromosome 10 present in cells. Here is what it examines:
| Parameter | What It Shows |
|---|---|
| Chromosome 10 copy number | Counts the number of chromosome 10 signals in each cell. Normally, two copies are present. Three or more signals indicate trisomy |
| Fluorescent signal count | A fluorescent probe binds to the centromeric region of chromosome 10, making it possible to count copies under a fluorescence microscope |
| Percentage of abnormal cells | The proportion of cells showing extra chromosome 10 copies is calculated across a large number of cells (up to 200 to 500 cells analysed per specimen) |
Why Is a Trisomy-10-FISH Test Done?
This test is requested when a doctor needs to detect chromosomal abnormalities linked to certain blood cancers. The findings help with diagnosis, risk classification and treatment planning.
Common Symptoms That May Require This Test
A doctor may request this test when a patient presents with the following symptoms:
- Unexplained and persistent fatigue
- Frequent or unusual infections
- Easy bruising or prolonged bleeding
- Bone pain without a clear cause
- Pallor (paleness of skin)
- Swollen lymph nodes (glands in the neck, armpits or groin)
- Unexplained weight loss
Conditions This Test Can Help Detect
The Trisomy-10-FISH test can help identify the following conditions:
- Paediatric acute lymphoblastic leukaemia (ALL), particularly the high-hyperdiploid subtype
- B-cell acute lymphoblastic leukaemia (B-ALL) and B lymphoblastic lymphoma in children
- Acute myeloid leukaemia (AML) in adults, in which chromosome 10 changes may be present
- Myelodysplastic syndromes and myeloproliferative disorders
- Certain brain tumours where chromosome 10 loss is relevant
- Rare congenital chromosomal disorders involving extra chromosome 10 material
Trisomy-10-FISH Test for Chronic Disease Monitoring
This test is not limited to initial diagnosis. For patients already diagnosed with leukaemia or myelodysplastic syndromes, the Trisomy-10-FISH test plays a role in tracking how well treatment is working. FISH analysis can be performed on both dividing and non-dividing cells, making it possible to estimate the proportion of abnormal cells before and after therapy. In ALL patients, chromosome 10 trisomy status established at diagnosis guides treatment intensity and can be reassessed to monitor disease response. It may also be used to detect minimal residual disease (MRD), meaning small amounts of leukaemia remaining after treatment.
How to Prepare and What to Expect
The preparation steps are straightforward since no fasting is required, but there are a few practical points to keep in mind before your appointment.
Do You Need to Fast?
No fasting is required before the Trisomy-10-FISH test. You may eat and drink normally on the day of the test. However, if your doctor advises sedation or any form of anaesthesia for the bone marrow collection, you may need to follow separate dietary instructions provided by your clinical team.
Practical Tips Before Your Test
Please keep the following in mind before attending your appointment:
- Bring a detailed clinical history, including your symptoms, previous test results and family history, as this is required for the test.
- Carry any previous cytogenetic studies, haematopathology reports or bone marrow examination records.
- Inform your doctor about all current medications, especially blood thinners or anticoagulants.
- Disclose any known allergies or existing health conditions before the procedure.
- Wear loose, comfortable clothing.
Step-by-Step Procedure
This test requires two types of samples: a bone marrow aspirate and a peripheral blood sample. Both may be collected during the same appointment.
Peripheral Blood Sample Collection:
- A trained phlebotomist cleans the skin over a vein, usually in the arm.
- A needle is used to collect approximately 3 ml of blood into a lavender-top EDTA tube.
- The needle is removed, and a small bandage or cotton swab is applied to the site.
- The sample is labelled and stored at 2 to 8 degrees Celsius for transport.
Bone Marrow Collection:
- A doctor performs the bone marrow collection, usually from the hip bone, under local anaesthesia or mild sedation.
- The skin over the collection site (usually the hip bone) is cleaned and numbed with a local anaesthetic.
- A small amount of bone marrow, approximately 3 ml, is drawn using a thin needle. This procedure is called bone marrow aspiration.
- The sample is placed in a green-capped sodium heparin tube and stored at 2 to 8 degrees Celsius for safe transport.
Both samples are sent to the cytogenetics laboratory, where the cells are spread onto glass slides and exposed to a fluorescent probe that binds to chromosome 10. A qualified cytogeneticist counts the fluorescent signals in multiple cells and prepares a detailed report, which is delivered within 8 days.
Factors That Can Affect Accuracy
The following factors may influence the reliability of your results:
- Poor sample quality or low cell viability
- A low percentage of blast (abnormal) cells in the specimen
- Use of the wrong anticoagulant tube during collection
- Delay in sample transport beyond 24 hours
- Clotted or frozen specimens, which are not acceptable
- Prior chemotherapy, which can affect cell viability
Understanding Your Trisomy-10-FISH Test Results
Results from this test must always be reviewed by a specialist, typically a haematologist, oncologist or clinical geneticist. The table below provides a general guide to result interpretation.
| Parameter | Finding | Interpretation |
|---|---|---|
| Chromosome 10 copy number | 2 signals per cell | Normal (disomy) |
| Chromosome 10 copy number | 3 or more signals per cell | Trisomy 10 detected (positive result) |
| Chromosome 10 copy number | 1 signal or absent | Monosomy or loss of chromosome 10 |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain factors can affect how results are interpreted:
- In patients above 60 years of age, trisomy 10 as a sole chromosomal abnormality has been associated with a less favourable outcome compared to younger patients.
- A low blast cell percentage in the sample may produce inconclusive results, and repeat testing may be recommended.
- Previous chemotherapy can reduce cell viability and affect the accuracy of the FISH analysis.
- FISH analysis is also limited to the specific chromosomal region tested and will not identify structural rearrangements or other numerical abnormalities elsewhere in the genome.
How to Maintain Healthy Levels
Chromosomal abnormalities are genetic in nature and are not influenced by diet or lifestyle. General guidance includes:
- Follow the treatment plan prescribed by your haematologist or oncologist without interruption.
- Attend all scheduled follow-up appointments so that disease status can be monitored over time.
- Seek genetic counselling if a chromosomal disorder is confirmed, particularly for families with young children.
- Maintain adequate hydration and a balanced diet to support your overall health during treatment.
Lupin Diagnostics Trisomy-10-FISH Test Price
The Trisomy-10-FISH test cost at Lupin Diagnostics starts at ₹4,400. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 4400 |
| CHENNAI | 4400 |
| HYDERABAD | 4400 |
| KOLKATA | 4400 |
| NAVI MUMBAI | 4400 |
| PUNE | 4400 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The Trisomy-10-FISH test online booking process at Lupin Diagnostics is straightforward:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
Trisomy 10 means a cell carries three copies of chromosome 10 instead of the usual two. The Trisomy-10-FISH test is primarily ordered in patients being evaluated for blood cancers, particularly acute lymphoblastic leukaemia, where the presence of this trisomy often points to a more favourable outcome with treatment.
In children with acute lymphoblastic leukaemia (ALL), trisomy 10 is associated with a favourable prognosis, particularly when trisomies of chromosomes 4 and 17 are also present. Identifying this abnormality helps doctors classify the patient into a favourable risk group and plan the appropriate level of treatment.
Local anaesthesia is used before the procedure to minimise discomfort. You may feel brief pressure or a short sharp sensation when the needle is inserted and the fluid is withdrawn. The discomfort usually passes quickly after the procedure. Your doctor can discuss the option of conscious sedation if you are concerned about discomfort.
The report is delivered within 8 days. Turnaround time may vary depending on sample quality and whether additional confirmatory analysis is required.
Yes, clinical history is required for this test. Please bring your medical records, including previous blood test results, any prior cytogenetic reports and a summary of your symptoms and diagnoses, when you visit the centre for sample collection.
A positive result means that three or more copies of chromosome 10 were detected in a significant proportion of cells. In the context of childhood ALL, this is generally a favourable finding. In adults, it may be linked to other haematological conditions. Your doctor will explain the clinical significance of the result in the context of your overall diagnosis.
Trisomy-10-FISH Test: Booking, Price, and Results
