TP53 Gene Analysis Test: Booking, Price, and Results
About TP53 Gene Analysis Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | TP53 mutation analysis, TP53 full gene analysis, TP53 somatic mutation, p53 gene testing, TP53 sequencing |
| Sample Type | Bone marrow (sodium heparin tube) and peripheral blood (EDTA tube) |
| Fasting Required | No fasting required |
| Report Time | 20 days |
| Recommended For | All genders and ages; individuals with a personal or family history of cancers linked to Li-Fraumeni syndrome |
| Price | Starting at ₹30,000 |
What Is a TP53 Gene Analysis Test?
The TP53 Gene Analysis test examines the TP53 gene, also called the tumour suppressor gene, for mutations that may be linked to cancer development. When this gene is altered, cells may multiply without control, which can lead to tumour formation. It is ordered for individuals with a personal history of cancer, a strong family history of early-onset cancers or a suspected inherited condition called Li-Fraumeni syndrome. Also known as TP53 mutation analysis or TP53 sequencing, this test uses bone marrow and peripheral blood samples analysed by next-generation sequencing (NGS).
What Does a TP53 Gene Analysis Test Measure?
This test looks for specific changes in the TP53 gene and its behaviour at a molecular level. The table below explains what is assessed.
| Component | What It Tells Us |
|---|---|
| TP53 gene mutation status | Whether a disease-causing change is present in the TP53 gene |
| p53 protein function | Whether the gene can still produce a protein that controls cell growth and DNA repair |
| Mutation types (point mutations, small insertions or deletions) | The precise nature of any genetic change identified |
| Variant of uncertain significance (VUS) | Flags a genetic change whose clinical impact is not yet fully understood |
| Variant allele frequency (VAF) | Estimates the proportion of cells in the sample that carry the mutation |
Why Is a TP53 Gene Analysis Test Done?
Doctors request this test for a range of clinical reasons, from investigating unexplained cancer patterns to guiding treatment decisions.
Common Symptoms That May Require This Test
The following situations or findings commonly prompt a doctor to recommend this test.
- Personal history of cancer diagnosed at an unusually young age
- Multiple different cancers occurring in the same individual
- Strong family history of cancers associated with Li-Fraumeni syndrome
- Childhood cancers such as bone tumours, soft tissue sarcomas or brain tumours
- Family members already found to carry a TP53 mutation
- Suspected treatment-resistant or rapidly progressing cancer
Conditions This Test Can Help Detect
Several conditions are associated with TP53 gene changes. This test can help identify the following.
- Li-Fraumeni syndrome (LFS), a hereditary condition that raises the risk of several cancer types
- Breast cancer and osteosarcoma (bone cancer) with a genetic basis
- Soft tissue sarcomas (cancers of muscle and connective tissue)
- Brain tumours, including medulloblastoma and IDH-mutant astrocytoma
- Leukaemias, particularly chronic lymphocytic leukaemia (CLL)
- Adrenocortical carcinoma (cancer of the adrenal glands)
TP53 Gene Analysis Test for Chronic Disease Monitoring
The TP53 Gene Analysis test plays an important role in managing chronic lymphocytic leukaemia (CLL). TP53 alterations are found in a higher proportion of patients with advanced or treatment-resistant CLL than in newly diagnosed cases, making re-testing necessary before each new line of treatment. Early and repeated testing helps track how the disease is evolving and guides treatment decisions.
How to Prepare and What to Expect
The TP53 Gene Analysis test procedure requires two sample types: peripheral blood and bone marrow. No special preparation is needed before this test, but a few practical steps will help the process go smoothly.
Do You Need to Fast?
No fasting is required for the TP53 Gene Analysis test. You can eat and drink normally on the day of sample collection.
Practical Tips Before Your Test
The following steps will help ensure accurate sample collection and processing.
- Bring a detailed clinical history, including your symptoms, previous test results and family history, as this is required for the test.
- Inform your doctor of all medications you are currently taking, including supplements.
- Tell your doctor about any known allergies before the procedure.
- Genetic counselling is strongly recommended before and after testing to help you understand the implications of results.
- Wear clothing with easy access to your arm for the blood draw.
- Stay well hydrated before your appointment.
Step-by-Step Procedure
This test requires two types of samples: peripheral blood and bone marrow. Both are collected at the diagnostic centre.
Peripheral Blood Collection
- A trained phlebotomist cleans the skin on your inner arm and inserts a small needle into a vein.
- A small amount of blood (around 3 ml) is drawn into a lavender-capped EDTA tube.
- You may feel a brief sting when the needle is inserted; the process usually takes under five minutes.
- The needle is removed, and a small bandage is placed over the site.
Bone Marrow Collection
- Bone marrow collection is performed by a specialist doctor at the centre under sterile conditions.
- The doctor will clean and numb a small area, usually at the back of the hip bone (iliac crest), using local anaesthetic.
- A small quantity of bone marrow (around 3 ml) is collected into a green-capped sodium heparin tube.
- Some pressure or discomfort may be felt during this step, but it typically lasts only a short time.
- The site is bandaged after collection.
Both samples are labelled, stored at 2 to 8 degrees Celsius, and dispatched to the NABL-accredited laboratory for analysis by next-generation sequencing (NGS). Results are prepared and delivered within 20 days.
Factors That Can Affect Accuracy
Several factors may influence the reliability of results. These include the following.
- Low tumour cell content in the sample, which can reduce detection sensitivity
- Mutations present at very low levels, which may fall below the detection limit of the assay
- Inadequate sample volume or poor sample quality
- Clonal haematopoiesis, an age-related change in blood cells that can complicate result interpretation
- The test does not detect large-scale gene deletions, duplications or epigenetic changes such as promoter methylation
Understanding Your TP53 Gene Analysis Test Results
Results from the TP53 Gene Analysis test are reported qualitatively as "mutation detected" or "mutation not detected", rather than as a numerical value, along with details of any variants identified. The table below outlines how results are typically classified.
| Result Category | Meaning |
|---|---|
| Mutation not detected | No harmful TP53 gene changes found in the tested sample |
| Pathogenic or likely pathogenic variant detected | A disease-causing or likely disease-causing change has been identified |
| Variant of uncertain significance (VUS) | A genetic change is present, but its clinical significance is not yet established |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
- In CLL, TP53 mutations become more frequent as the disease progresses, so a result at diagnosis may not reflect the status at a later stage. Re-testing before starting each new treatment line is therefore recommended.
- Some TP53 mutations that appear inherited may actually be somatic, meaning they are present in only a subset of cells. This distinction matters because somatic mutations in tumour cells alone do not indicate Li-Fraumeni syndrome.
- In roughly 5% of cases, a variant of uncertain significance is found. Its relationship to cancer risk may be clarified by future research.
How to Maintain Healthy Levels
This is a genetic test rather than a biochemistry test. It does not produce a result that can be "improved" through lifestyle changes. However, the following steps are relevant for those with a confirmed TP53 mutation:
- Discuss a personalised cancer screening schedule with your doctor, as early detection is important for people with hereditary TP53 changes.
- Avoid unnecessary radiation exposure, particularly for individuals with an inherited TP53 mutation.
- Maintain regular follow-up appointments with your genetic counsellor and oncologist.
Lupin Diagnostics TP53 Gene Analysis Test Price
The TP53 Gene Analysis test price starts at ₹30,000 at Lupin Diagnostics. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test due to the specialised nature of sample collection.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 30000 |
| CHENNAI | 30000 |
| HYDERABAD | 30000 |
| KOLKATA | 30000 |
| NAVI MUMBAI | 30000 |
| PUNE | 30000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The TP53 Gene Analysis test online booking process at Lupin Diagnostics is simple:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The TP53 gene produces a protein that normally stops cells from dividing too quickly, helps repair damaged DNA and triggers cell death when damage cannot be repaired. When this gene carries a harmful mutation, these controls can fail, allowing abnormal cells to grow. The TP53 Gene Analysis test identifies such mutations to inform cancer diagnosis and treatment.
This test is typically recommended for people with a personal or family history of early-onset cancers, multiple cancers in one individual or cancers associated with Li-Fraumeni syndrome. It is also used in people already diagnosed with cancer to help select the most appropriate treatment approach.
Li-Fraumeni syndrome is a rare hereditary condition caused by an inherited change in one copy of the TP53 gene. It significantly raises the likelihood of developing several types of cancer, sometimes from childhood onwards. The condition is estimated to affect roughly 1 in 5,000 to 1 in 20,000 people worldwide.
No. A TP53 mutation found in a tumour sample is usually a somatic change (acquired during the person's lifetime) and does not mean the mutation was inherited. Li-Fraumeni syndrome is caused by inherited TP53 mutations. Your doctor and genetic counsellor will help distinguish between the two based on your full clinical picture.
At Lupin Diagnostics, the report is delivered within 20 days. Turnaround times vary depending on the laboratory and the specific analysis method used.
The blood draw is generally quick and causes only a brief sting. The bone marrow collection is done under local anaesthetic, so the area is numbed beforehand. Some patients feel pressure during bone marrow aspiration, but this passes quickly. Your doctor will explain what to expect beforehand and address any concerns.
Yes. Close relatives such as parents, siblings and children have the highest likelihood of carrying the same inherited mutation. Testing at-risk family members allows early identification of those who may benefit from regular cancer screening and preventive follow-up. Speak with a genetic counsellor for guidance on family testing.
Always discuss your results with your treating doctor or a genetic counsellor. A positive or uncertain finding does not automatically indicate cancer; further clinical assessment is needed. For those with confirmed Li-Fraumeni syndrome, a tailored surveillance plan and avoidance of unnecessary radiation exposure are generally advised.
TP53 Gene Analysis Test: Booking, Price, and Results
