TERT Gene Analysis (C228T and C250T) Test: Booking, Price, and Results
About TERT Gene Analysis (C228T and C250T) Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | TERT Promoter Mutation Analysis, TERTp Mutation Test, Telomerase Reverse Transcriptase Promoter Mutation Test, TERT C228T/C250T Analysis |
| Sample Type | Peripheral blood (EDTA tube) |
| Fasting Required | No fasting required |
| Report Time | 15 days |
| Recommended For | Patients with suspected or confirmed cancers, particularly brain tumours, bladder cancer, thyroid cancer, melanoma or hepatocellular carcinoma |
| Price | Starting at ₹10,800 |
What Is a TERT Gene Analysis (C228T and C250T) Test?
The TERT Gene Analysis (C228T and C250T) test is a molecular genetic test that looks for two specific mutations in the promoter region of the TERT gene. TERT stands for telomerase reverse transcriptase, the enzyme responsible for maintaining chromosome length in cells. When mutations occur in this gene's promoter region, telomerase activity increases, allowing cancer cells to keep dividing unchecked.
This test is also known as TERT Promoter Mutation Analysis or the TERTp Mutation Test. It is typically ordered as part of a cancer workup to support diagnosis and assess prognosis. A blood sample is collected for this analysis.
What Does a TERT Gene Analysis (C228T and C250T) Test Measure?
The TERT Gene Analysis test looks for two specific DNA changes in the promoter region of the TERT gene. A promoter region controls how actively a gene is switched on. Here is what each mutation means:
| Mutation | Location | Effect |
|---|---|---|
| C228T (c.-124C>T) | 124 base pairs before the gene's start site | Creates a new binding site that switches on telomerase activity |
| C250T (c.-146C>T) | 146 base pairs before the gene's start site | Similarly activates telomerase expression in tumour cells |
Together, these two mutations account for over 98% of all TERT promoter mutations seen across cancer types. When either is present, telomerase activity in tumour cells rises significantly, allowing those cells to avoid normal ageing and continue dividing.
Why Is a TERT Gene Analysis (C228T and C250T) Test Done?
This test is ordered when a doctor needs more information about a suspected or confirmed tumour. It helps assess malignant potential and provides important prognostic information.
Common Symptoms That May Require This Test
A doctor may order the TERT Gene Analysis test when a patient presents with any of the following:
- Persistent headaches, seizures or neurological changes that suggest a brain tumour
- Blood in the urine (haematuria), which may indicate bladder cancer
- A suspicious thyroid nodule found on imaging or biopsy
- An unusual skin lesion or pigmented growth that may be melanoma
- Abnormal liver imaging findings that could suggest hepatocellular carcinoma
- Pre-operative cancer staging where mutation status will guide treatment decisions
Conditions This Test Can Help Detect
The TERT C228T/C250T analysis is relevant across several cancer types. This test does not diagnose cancer on its own. It identifies mutation status that helps characterise the following tumour types:
- Brain tumours, particularly gliomas and glioblastomas
- Bladder cancer
- Melanoma
- Hepatocellular carcinoma (liver cancer)
- Thyroid cancers, particularly more aggressive subtypes
- Basal cell carcinoma
How to Prepare and What to Expect
Preparation for the TERT Gene Analysis (C228T and C250T) test procedure is straightforward. No special steps are needed before a blood draw.
Do You Need to Fast?
No. Fasting is not required for this test. You may eat and drink normally before sample collection.
Practical Tips Before Your Test
A smooth collection and accurate result depend on a few simple steps. Keep the following in mind:
- Bring a detailed clinical history, including your symptoms, previous test results and family history, as this is required for the test.
- Inform your doctor about any previous biopsies or surgical samples that may already be available, as these may be used for analysis.
- Disclose all medications and supplements to your doctor before the test.
- Wear a loose-fitting top or short sleeves for easy access during blood collection.
- Stay well hydrated before the appointment, as this makes the blood draw easier.
Step-by-Step Procedure
The TERT Gene Analysis test procedure involves the following steps:
- A trained phlebotomist cleans the skin on your inner arm and draws 2 ml of blood into a lavender-top EDTA tube.
- The sample is labelled and stored at a refrigerated temperature of 2 to 8 degrees Celsius to maintain DNA integrity during transport.
- At the laboratory, genomic DNA is extracted from your blood sample.
- The TERT gene's promoter region is amplified using PCR (a technique that makes many copies of a specific DNA segment for analysis).
- The amplified DNA is then analysed using Sanger sequencing to identify whether the C228T or C250T mutation is present.
- A qualified pathologist reviews the findings and prepares an interpretive report, which is delivered within 15 days.
Factors That Can Affect Accuracy
Several factors can influence the reliability of results:
- Quality and quantity of DNA extracted from the sample
- The proportion of tumour-derived DNA present in a blood-based sample
- Sample handling and storage conditions during transport
- The high guanine-cytosine content (over 80%) of the TERT promoter region, which can make amplification technically challenging
- Mutations present at very low levels may fall below the assay's detection threshold
Understanding Your TERT Gene Analysis (C228T and C250T) Test Results
Results from this test are reported as either negative (no mutation detected) or positive (mutation present) for a specific mutation. Your doctor will review these findings alongside imaging, histopathology and your overall clinical picture.
| Result | Interpretation |
|---|---|
| Negative (no mutation detected)/wild-type | No C228T or C250T mutation detected in the sample |
| Positive: C228T detected | Mutation present; associated with increased telomerase activity and tumour |
| Positive: C250T detected | Mutation present; associated with increased telomerase activity and tumour |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history and other factors. Always consult a qualified healthcare professional for personalised medical advice.
How to Maintain Healthy Levels
This test identifies genetic mutations in tumour cells rather than measuring a lifestyle-modifiable value. General cancer-prevention habits that support overall health include:
- Avoiding excessive UV exposure to reduce the risk of melanoma and skin damage
- Maintaining a healthy body weight through a balanced diet and regular physical activity
- Following recommended cancer screening guidelines and attending regular follow-up appointments with your oncologist
Lupin Diagnostics TERT Gene Analysis (C228T and C250T) Test Price and Home Collection
The TERT Gene Analysis test cost at Lupin Diagnostics starts at ₹10,800, with home sample collection available across cities. The table below lists indicative prices by city.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 10800 |
| CHENNAI | 10800 |
| HYDERABAD | 10800 |
| KOLKATA | 10800 |
| NAVI MUMBAI | 10800 |
| PUNE | 10800 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The TERT Gene Analysis test online booking process at Lupin Diagnostics is simple:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Home Collection
The TERT Gene Analysis test home collection is available across multiple cities through Lupin Diagnostics. A certified phlebotomist visits your home at your chosen time to collect the blood sample. All samples are processed in NABL-accredited laboratories, and your reports are delivered digitally via email or WhatsApp.
Frequently Asked Questions
The TERT gene encodes the enzyme telomerase, which normally maintains chromosome length during cell division. When mutations occur in the TERT gene's promoter region, telomerase becomes overactive, allowing cancer cells to divide indefinitely. This is one of the key processes that drives tumour growth and progression.
TERT promoter mutations are found in a wide range of cancers. They are most frequently detected in glioblastoma (approximately 83%), melanoma (approximately 71%), bladder cancer (approximately 66%) and hepatocellular carcinoma (approximately 47%). They are also present in thyroid cancers, particularly the more aggressive subtypes.
Yes. At Lupin Diagnostics, the TERT Gene Analysis test procedure uses a peripheral blood sample. Blood-based testing allows detection of tumour-derived DNA circulating in the bloodstream, making it a practical option for many patients.
No. A negative result means that the C228T and C250T mutations were not detected in your sample. It does not rule out cancer entirely, as the absence of these specific mutations does not exclude malignancy. Your doctor will interpret this result in context with all other diagnostic findings.
The report is typically delivered within 15 days from the date of sample collection at Lupin Diagnostics. This timeframe reflects the technical complexity of Sanger sequencing and the expert review required for interpretation.
Clinical history helps the pathologist interpret results accurately. Information about your symptoms, prior diagnoses, biopsy reports and treatment history provides essential context that influences how the mutation findings are understood and reported in relation to your specific condition.
TERT Gene Analysis (C228T and C250T) Test: Booking, Price, and Results
