TB DNA PCR, Qualitative Test: Booking, Price, and Results
About TB DNA PCR, Qualitative Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | TB PCR, Tuberculosis PCR, MTB DNA PCR, Mycobacterium Tuberculosis DNA Detection, TB DNA Amplification Test, Tuberculosis Nucleic Acid Amplification Test (NAAT) |
| Sample Type | BAL (Bronchoalveolar Lavage) or Ascitic Fluid, collected in a sterile container |
| Fasting Required | No fasting required |
| Report Time | 2 days |
| Recommended For | All age groups, both males and females |
| Price | Starting at ₹1,950 |
What Is a TB DNA PCR, Qualitative Test?
The TB DNA PCR, Qualitative test is a molecular diagnostic test that detects the genetic material (DNA) of Mycobacterium tuberculosis, the bacteria responsible for tuberculosis. It uses a technique called Real Time PCR with a Taqman probe to identify TB bacteria DNA directly in a body fluid sample. Also known as Tuberculosis PCR or MTB DNA PCR, this test is ordered when a doctor suspects active TB infection, particularly at sites outside the lungs such as the respiratory tract or abdomen. The sample used is BAL (bronchoalveolar lavage) fluid or ascitic fluid, collected under medical supervision.
What Does a TB DNA PCR, Qualitative Test Measure?
This test analyses the sample for the presence or absence of specific DNA sequences belonging to the Mycobacterium tuberculosis complex. The result is qualitative, meaning it tells you whether TB bacteria DNA was detected or not detected. The following targets are assessed:
| Component | What It Tells You |
|---|---|
| M. tuberculosis complex DNA | Whether tuberculosis bacteria are present in the sample |
| Non-tuberculous Mycobacteria (NTM) DNA | Whether atypical mycobacteria (bacteria related to TB but distinct from it) are present |
Why Is a TB DNA PCR, Qualitative Test Done?
A doctor may order this TB DNA PCR, Qualitative test when a patient presents with symptoms or clinical findings that raise concern for tuberculosis, especially at extrapulmonary sites.
Common Symptoms That May Require This Test
The following symptoms are among the most common reasons a doctor may recommend this test:
- Persistent cough lasting more than two to three weeks
- Coughing up blood or blood-stained sputum
- Unexplained weight loss
- Fever and chills
- Night sweats
- Chest pain or difficulty breathing
- Fatigue or general weakness
Conditions This Test Can Help Detect
This test can assist in identifying several conditions, including:
- Pulmonary tuberculosis (TB affecting the lungs)
- Extrapulmonary tuberculosis (TB in lymph nodes, bones, brain, kidneys, or abdomen)
- Non-tuberculous Mycobacteria (NTM) infections, which are caused by related bacteria and can affect the lungs, skin, and soft tissue
How to Prepare and What to Expect
No special preparation is needed before a TB DNA PCR, Qualitative test. The section below covers everything you should know before your appointment.
Do You Need to Fast?
No, fasting is not required for this test. You may eat and drink normally before your sample is collected.
Practical Tips Before Your Test
Keep the following in mind before your test appointment:
- Bring a detailed clinical history, including your symptoms, previous test results, and relevant medical records, as this is required for the test.
- Inform your doctor about all medications and supplements you are currently taking, as some may affect the result.
- Wear comfortable clothing with easy access to the area where the sample will be collected.
- If you have had TB treatment in the past, inform your doctor before the test.
Step-by-Step Procedure
This test requires a BAL (bronchoalveolar lavage) fluid or ascitic fluid sample, collected by a trained medical professional. Here is what the process involves:
- Your doctor reviews your clinical history before proceeding with sample collection.
- The collection procedure (bronchoscopy for BAL or abdominal aspiration for ascitic fluid) is carried out by a trained clinician in a clinical setting.
- At least 5 ml of fluid is collected and transferred into a sterile container with a white cap.
- The container is labelled with your name and details, then stored under refrigeration (2 to 8 degrees Celsius) for transport.
- The sample is sent to the laboratory, where DNA is extracted and amplified using Real Time PCR with a Taqman probe.
- The probe detects the presence or absence of TB DNA, and the result is reported as detected or not detected.
Factors That Can Affect Accuracy
Several factors can influence the reliability of this test:
- Sample quality and volume (insufficient fluid may reduce sensitivity)
- Presence of natural inhibitors in the sample that interfere with PCR
- Prior antitubercular treatment, which can lower the bacterial load and reduce sensitivity
- Laboratory contamination prevention practices
- The specific site and extent of infection
Understanding Your TB DNA PCR, Qualitative Test Results
Your results will be reported as either "Detected" or "Not Detected." A doctor should always review your results in the context of your symptoms, clinical history, and other investigations.
| Parameter | Result | Interpretation |
|---|---|---|
| M. tuberculosis DNA | Not Detected (Negative) | No TB bacteria DNA found; TB infection is unlikely based on this sample |
| M. tuberculosis DNA | Detected (Positive) | TB bacteria DNA is present; suggests active or recent TB infection |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain conditions may alter how results are interpreted:
- Patients currently on antitubercular therapy may show a negative result even when TB is present, due to reduced bacterial levels in the sample.
- A person with a history of successfully treated TB may return a positive result for several years after completing treatment, as the test detects DNA rather than live bacteria.
- Low sample volumes can reduce sensitivity and may lead to a false negative result.
How to Maintain Healthy Levels
These general wellness tips may help reduce TB exposure risk:
- Avoid close or prolonged contact with individuals known to have active tuberculosis.
- Ensure good ventilation in living and working spaces to reduce airborne transmission risk.
- Seek prompt medical attention if you develop a persistent cough, unexplained weight loss, or night sweats.
Lupin Diagnostics TB DNA PCR, Qualitative Test Price and Home Collection
The TB DNA PCR, Qualitative test is available at Lupin Diagnostics starting at ₹1,950. Please note that this is a specialised test requiring clinical sample collection at a medical facility; the sample must be collected by a trained clinician before being sent to a Lupin Diagnostics centre or picked up for processing.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1950 |
| CHENNAI | 2150 |
| HYDERABAD | 1950 |
| KOLKATA | 1950 |
| NAVI MUMBAI | 1950 |
| PUNE | 1950 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your TB DNA PCR, Qualitative test online:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Home Collection
Lupin Diagnostics offers sample collection support across cities for the TB DNA PCR, Qualitative test. All samples are processed in NABL-accredited laboratories by experienced professionals. Once processed, your digital report is shared promptly via email or WhatsApp.
Frequently Asked Questions
The TB DNA PCR, Qualitative test is used to detect the genetic material of Mycobacterium tuberculosis in body fluid samples such as BAL or ascitic fluid. It helps confirm or rule out active TB infection, particularly at extrapulmonary sites. This test is especially useful when a rapid result is needed.
The TB DNA PCR test detects active TB by identifying actual bacterial DNA in a sample. In contrast, the tuberculin skin test and IGRA (interferon gamma release assay) detect the body's immune response and are used to identify latent (dormant) TB. This test should not be used as a substitute for latent TB testing.
At Lupin Diagnostics, reports are typically delivered within 2 days of sample collection. This is significantly faster than traditional TB culture methods, which can take several weeks. Rapid results help doctors begin appropriate care sooner.
No. A negative result means TB DNA was not detected in the sample tested, but it does not fully exclude a TB diagnosis. If clinical suspicion remains high, your doctor may recommend further tests such as culture, imaging, or biopsy.
Clinical history is required because the interpretation of TB DNA PCR results depends heavily on the patient's background, including symptoms, prior TB episodes, and any ongoing treatment. It also helps the laboratory flag relevant considerations when processing the sample.
Yes, the test is safe for pregnant women when TB is clinically suspected. Early detection is important to avoid complications for both the mother and the baby. Your doctor will advise whether this test is appropriate for your situation.
The standard TB DNA PCR, Qualitative test confirms whether TB DNA is present but does not assess drug resistance. Some advanced PCR platforms can additionally detect mutations linked to rifampicin resistance. Your doctor may order a supplementary test if drug-resistant TB is suspected.
TB DNA PCR, Qualitative Test: Booking, Price, and Results
