PGT SR – 5 Embryos Test: Booking, Price, and Results
About PGT SR – 5 Embryos Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | PGT-SR, Preimplantation Genetic Testing for Structural Rearrangements, PGD for translocations/inversions |
| Sample Type | Embryo biopsy (trophectoderm cells from blastocyst-stage embryos, Day 5 or Day 6) |
| Fasting Required | Not applicable — this is an embryo laboratory procedure, not a patient blood test |
| Report Time | 18 days from receipt of samples |
| Recommended For | Couples where one or both partners carry a balanced chromosomal rearrangement (translocation or inversion); couples with recurrent pregnancy loss |
| Price | Starting at ₹72,000 |
What Is a PGT SR – 5 Embryos Test?
The PGT SR – 5 Embryos test is a specialised genetic test performed on embryos created through in vitro fertilisation (IVF). PGT-SR stands for Preimplantation Genetic Testing for Structural Rearrangements. It checks embryos for chromosomal imbalances that result from a structural change in one or both parents' chromosomes. This test is used before an embryo is transferred to the uterus. It is recommended for couples carrying balanced chromosomal rearrangements such as translocations or inversions. The test is performed on embryo biopsy samples using Next-Generation Sequencing (NGS), a method that reads the genetic information with high accuracy.
What Does a PGT SR – 5 Embryos Test Measure?
The PGT SR – 5 Embryos test procedure analyses trophectoderm cells (a few cells taken from the outer layer of the embryo) to assess the chromosomal status of each embryo. The following table summarises what the test evaluates.
| Parameter Assessed | What It Means |
|---|---|
| Structural chromosomal imbalances | Detects extra or missing chromosome material arising from a parental translocation or inversion |
| Balanced rearrangements | Identifies embryos carrying the parental rearrangement in a balanced form (no gain or loss of material) |
| Aneuploidy (numerical errors) | Detects embryos with an incorrect total number of chromosomes, in addition to structural assessment |
| Mosaic findings | Identifies embryos where some cells have a different chromosomal pattern from others |
The test covers both structural and numerical chromosomal abnormalities, giving a picture of each embryo's genetic suitability for transfer.
Why Is a PGT SR – 5 Embryos Test Done?
Couples with chromosomal rearrangements face a higher risk of passing on unbalanced chromosomes to their embryos. The PGT SR – 5 Embryos test procedure helps identify which embryos are suitable for transfer, reducing the risk of implantation failure or pregnancy loss.
Common Symptoms That May Require This Test
This test is not triggered by symptoms in the usual sense. Instead, it is indicated based on reproductive and genetic history. Situations that commonly lead to a referral for PGT-SR testing include:
- Two or more unexplained miscarriages (recurrent pregnancy loss)
- A known balanced chromosomal translocation or inversion in either partner
- Repeated IVF failure without a clear cause
- A previous pregnancy or child affected by a chromosomal imbalance
- A family history of chromosomal structural rearrangements
- Abnormal results on parental karyotype (chromosome blood test)
- Unexplained infertility combined with a family history of chromosomal conditions
Conditions This Test Can Help Detect
The PGT SR – 5 Embryos test helps identify the following chromosomal situations in embryos:
- Unbalanced reciprocal translocations (where two chromosomes exchange segments unevenly)
- Unbalanced Robertsonian translocations (where two specific chromosomes fuse)
- Chromosomal inversions resulting in unbalanced embryos
- Complex chromosomal rearrangements
- Numerical chromosome abnormalities (aneuploidies) across all chromosomes
How to Prepare and What to Expect
The PGT SR – 5 Embryos test is carried out in a fertility laboratory as part of an IVF cycle. There is no patient fasting or physical preparation required for the test itself. However, several steps must be completed before the embryo biopsy samples are sent for analysis. The preparation steps below apply to the couple undergoing IVF and PGT-SR.
Do You Need to Fast?
No fasting is required. This test is performed on embryo biopsy samples in a laboratory setting. It is not a blood or urine test for the patient.
Practical Tips Before Your Test
Before proceeding with the PGT SR – 5 Embryos test, ensure the following are in order:
The following steps are essential before the embryo biopsy and genetic analysis can take place:
- Attend genetic counselling sessions to understand what the results may mean for your family.
- Provide a copy of your parental karyotype report (chromosome analysis from a blood test), which is typically required before starting PGT-SR.
- Bring a detailed clinical history, including your symptoms, previous test results and family history, as this is required for the test.
- Submit a duly completed Test Request Form (TRF), biopsy worksheet and signed consent form as instructed by your clinic.
- Confirm with your fertility clinic that ICSI (intracytoplasmic sperm injection, where a single sperm is injected directly into an egg) will be used as the fertilisation method, as this is the preferred fertilisation method for PGT-SR.
Step-by-Step Procedure
The following steps describe how the embryo biopsy sample is collected and processed for the PGT SR – 5 Embryos test:
- Eggs are retrieved from the female partner as part of a standard IVF cycle and fertilised with sperm in the laboratory using ICSI.
- The fertilised eggs are grown in the lab until they reach the blastocyst stage, which occurs on Day 5 or Day 6 after fertilisation.
- A trained embryologist carefully removes 5 to 6 cells from the trophectoderm (the outer cell layer of the embryo, which later becomes the placenta) using a microsurgical needle. The inner cell mass, which becomes the foetus, is not disturbed.
- After the biopsy, each embryo is immediately vitrified (rapidly frozen) to preserve it while awaiting results.
- The biopsied cells are placed in the PGT Collection Kit and transported on dry ice (-20°C) to the Lupin Diagnostics laboratory for NGS analysis.
- The laboratory performs whole-genome amplification, library preparation, sequencing and data analysis to generate the chromosomal report. Results are reported within 18 days of sample receipt.
Factors That Can Affect Accuracy
Several factors may influence the reliability of the test result:
- Quality and quantity of trophectoderm cells collected during biopsy
- Embryo mosaicism, where cells within the same embryo carry different chromosomal content
- Transport conditions, including whether the sample was kept on dry ice throughout transit
- Laboratory accreditation and technical experience
- Complexity of the parental chromosomal rearrangement
Understanding Your PGT SR – 5 Embryos Test Results
Results from the PGT SR – 5 Embryos test are reported per embryo. Your fertility specialist and genetic counsellor will review each embryo's chromosomal status and advise on which embryos are suitable for transfer. The table below outlines the general categories of results.
| Chromosomal Status | Interpretation |
|---|---|
| Euploid or Balanced | Normal chromosomal structure; embryo considered suitable for transfer |
| Unbalanced or Aneuploid | Chromosomal gain or loss detected; embryo not recommended for transfer |
| Mosaic | Mixed chromosomal findings; requires specialist counselling before a transfer decision |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain factors can affect how many usable embryos a couple obtains from a single IVF cycle:
- Couples with balanced translocations may need an average of 4 to 5 blastocysts to have a reasonable chance of obtaining at least one euploid (chromosomally normal) embryo, and multiple retrieval cycles may be needed.
- Advanced maternal age or low ovarian reserve can further reduce the proportion of chromosomally normal embryos available for transfer.
- Couples with complex chromosomal rearrangements tend to have a lower proportion of balanced or normal embryos, with some studies reporting rates below 6%.
- Female carriers of such rearrangements generally have a lower rate of transferable embryos compared with male carriers.
How to Maintain Healthy Levels
While chromosomal outcomes cannot be changed through lifestyle, the following general steps support the overall IVF process:
- Work closely with both your fertility specialist and genetic counsellor at every stage of the process.
- Discuss all reproductive options openly with your medical team, including the possibility of multiple IVF cycles.
- Prioritise overall health and wellbeing during fertility treatment, as advised by your doctor.
- Maintain a balanced diet and healthy body weight, as recommended by your fertility team.
- Avoid smoking and alcohol, which can affect egg and sperm quality.
Lupin Diagnostics PGT SR – 5 Embryos Test Price
The PGT SR – 5 Embryos test cost starts at ₹72,000. This test requires a visit to a Lupin Diagnostics centre or coordination through your fertility clinic. Home collection is not available for this test, as sample collection is performed in a fertility laboratory by a trained embryologist.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 72000 |
| CHENNAI | 72000 |
| HYDERABAD | 72000 |
| KOLKATA | 72000 |
| NAVI MUMBAI | 72000 |
| PUNE | 72000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps for PGT SR – 5 Embryos test online booking:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for coordination of sample collection with your IVF clinic and submission of required documentation.
- Receive your report via email or WhatsApp within 18 days from receipt of the biopsy samples.
Frequently Asked Questions
PGT-SR is used when one or both parents carry a structural chromosomal rearrangement, such as a translocation or inversion. PGT-A (Preimplantation Genetic Testing for Aneuploidies) screens embryos for the wrong number of chromosomes but does not specifically assess structural rearrangements. The PGT SR – 5 Embryos test also detects aneuploidies alongside structural findings, making it a broader assessment for couples with known rearrangements.
This test is recommended for couples where one or both partners carry a confirmed balanced chromosomal rearrangement, such as a reciprocal or Robertsonian translocation or an inversion. It is also considered for couples who have experienced recurrent pregnancy loss or repeated IVF failure without an identified cause. A genetic counsellor can help determine whether PGT-SR testing is the right choice for your situation.
The report is delivered within 18 days of the laboratory receiving the embryo biopsy samples. Your fertility specialist will then review the results with you and discuss the next steps in your IVF cycle.
Blastocyst-stage biopsy, performed on Day 5 or Day 6, is considered the preferred approach because it provides more cells for analysis and is less disruptive to embryo development than earlier biopsy methods. Neonatal outcomes following trophectoderm biopsy have been reassuringly documented in published studies. Your fertility clinic will discuss any specific concerns before proceeding.
The proportion of chromosomally balanced or normal embryos varies depending on the type of parental rearrangement. Studies show that couples with inversions tend to have the highest proportion of transferable embryos (around 44%), followed by those with Robertsonian translocations (around 39%) and those with reciprocal translocations (around 22%). Multiple IVF cycles may be needed to obtain at least one suitable embryo.
No. While the test significantly reduces the risk of transferring an embryo with a chromosomal imbalance, it cannot guarantee a successful pregnancy. Chromosomally normal embryos may still fail to implant or result in a miscarriage for reasons unrelated to chromosomal structure. Your specialist may also recommend confirmatory prenatal testing (such as CVS or amniocentesis) after a successful transfer.
Before submitting samples, you must provide a duly filled Test Request Form (TRF), a biopsy worksheet, a signed consent form and a detailed clinical history, including previous genetic and fertility reports. Parental karyotype results are also typically required. Ensuring all paperwork is complete before sample dispatch helps avoid delays in processing the PGT SR – 5 Embryos test.
PGT SR – 5 Embryos Test: Booking, Price, and Results
