PGT SR - 4 Embryos Test: Booking, Price, and Results
About PGT SR - 4 Embryos Test
| Field | Value |
|---|---|
| Also Known As | PGT-SR, Preimplantation Genetic Testing for Structural Rearrangements, PGD for Translocations, Embryo Chromosomal Rearrangement Testing |
| Sample Type | Embryo biopsy (trophectoderm cells from Day 5 blastocyst) |
| Fasting Required | No (sample is taken from the embryo, not the patient) |
| Report Time | 18 days |
| Recommended For | Couples where one or both partners carry a balanced chromosomal rearrangement (translocation or inversion); all genders |
| Price | Starting at ₹57,500 |
What Is a PGT SR - 4 Embryos Test?
The PGT SR - 4 Embryos Test is a genetic screening test performed on embryos created through in vitro fertilisation (IVF). It analyses up to four embryos for chromosomal imbalances caused by a structural rearrangement carried by one or both parents. The test uses Next Generation Sequencing (NGS), a method that reads the genetic material of each embryo in detail. It is also known as PGT-SR or Preimplantation Genetic Testing for Structural Rearrangements.
What Does a PGT SR - 4 Embryos Test Measure?
The PGT SR - 4 Embryos Test procedure examines each embryo's chromosomes for specific types of genetic imbalance. The table below explains what each result category means.
| Parameter | What It Means |
|---|---|
| Unbalanced Translocations | Extra or missing chromosomal material resulting from a parental reciprocal or Robertsonian translocation |
| Unbalanced Inversions | A chromosomal imbalance that may arise from a parental inversion, resulting in extra or missing genetic material in the embryo |
| Aneuploidy | Extra or missing whole chromosomes, which may occur in addition to abnormalities related to the parental rearrangement |
| Mosaicism | Evidence of both chromosomally normal and abnormal cell populations within the same embryo |
Why Is a PGT SR - 4 Embryos Test Done?
Couples who carry a chromosomal rearrangement face a higher risk of failed implantation, recurrent miscarriage, or having a child with a chromosomal disorder. This test screens embryos before transfer to identify those most likely to result in a healthy pregnancy.
Common Symptoms That May Require This Test
The following indications are among the most common reasons a fertility specialist may recommend this test:
- Recurrent miscarriages with no other identified cause
- Repeated IVF cycle failures despite good embryo quality
- Known carrier status for a chromosomal translocation or inversion
- Family history of chromosomal abnormalities
- The previous pregnancy was affected by a chromosomal disorder
- Difficulty conceiving despite multiple treatment cycles
Conditions This Test Can Help Detect
This test helps identify embryos affected by the following conditions:
- Unbalanced reciprocal translocation (chromosomal segments swapped between two chromosomes)
- Unbalanced Robertsonian translocation (two chromosomes fused)
- Chromosomal inversion causing loss or gain of genetic material
- Complex chromosomal rearrangements involving multiple chromosomes
- General chromosomal aneuploidy (too many or too few chromosomes)
How to Prepare and What to Expect
Because the sample is taken from the embryo rather than from the patient directly, preparation for the PGT SR - 4 Embryos Test is different from a standard blood test. Several steps must be completed before the embryo biopsy takes place.
Do You Need to Fast?
No fasting is required for this test. The sample is a small number of cells biopsied from the embryo, not a blood or urine sample from the patient.
Practical Tips Before Your Test
The following preparation steps are required or strongly advised before the test:
- Submit a karyotype report confirming the specific chromosomal rearrangement carried by you or your partner before the test can begin
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Bring a duly filled Test Request Form (TRF) and biopsy worksheet to the fertility centre
- Ensure a signed consent form is completed before the biopsy procedure
- Attend genetic counselling with a qualified medical geneticist before starting your IVF cycle
- Inform your fertility team of all current medications
Step-by-Step Procedure
- Your embryos are created in the laboratory through standard IVF fertilisation.
- The embryos are allowed to develop for approximately five days until they reach the blastocyst stage, a point where they contain over 100 cells.
- An embryologist removes five to six trophectoderm cells (the outer layer that later becomes the placenta) from each embryo. The inner cells that develop into the baby are not disturbed.
- Each embryo is frozen and stored safely while the biopsy samples are prepared and sent to the genetics laboratory using dry ice at -20 degrees Celsius.
- The laboratory analyses the samples using NGS technology to check for chromosomal imbalances.
- Results are reported within 18 days. Your fertility specialist then reviews the findings and advises on which embryo is suitable for transfer.
Factors That Can Affect Accuracy
Several factors may influence the reliability of the test result:
- Quality and developmental stage of the embryo at the time of biopsy
- Timing of the biopsy (Day 5 or Day 6 is preferred)
- The embryologist's experience and laboratory technique
- Quality of the whole genome amplification process (the method used to copy the small amount of DNA in the biopsy)
- Mosaicism within the embryo, where different cells carry different chromosomal profiles
Understanding Your PGT SR - 4 Embryos Test Results
Results are reported per embryo and fall into one of the following categories. Your fertility specialist and genetic counsellor will review these findings with you before any transfer decision is made.
| Result Category | Classification | What It Indicates |
|---|---|---|
| Euploid or Balanced | Normal / Balanced | No detectable chromosomal imbalance; generally considered suitable for transfer |
| Aneuploid or Unbalanced | Abnormal | Extra, missing, or unbalanced chromosomal material detected; generally not selected for transfer |
| Mosaic | Intermediate | Evidence of both normal and abnormal chromosomal cell populations requires specialist review and counselling |
| No Result | Inconclusive | No reliable result obtained due to insufficient DNA or technical limitations; repeat testing or re-biopsy may be considered |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Some conditions can affect how results are interpreted:
- The test cannot detect uniparental disomy, a condition where both copies of a chromosome come from one parent instead of one from each.
- The test does not screen for single-gene disorders such as cystic fibrosis, sickle cell anaemia, or Tay-Sachs disease. A separate test (PGT-M) is needed for those conditions.
- A general risk of birth defects of approximately 4 to 6% exists for all babies born following IVF, regardless of PGT-SR results.
- Mosaicism in an embryo may result in an interpretation less straightforward; a specialist discussion is needed in these cases.
How to Maintain Healthy Levels
These general tips support a positive outcome after embryo transfer:
- Follow all medication and monitoring protocols prescribed by your fertility specialist.
- Attend all scheduled follow-up appointments after embryo transfer.
- Consider confirmatory prenatal testing such as CVS or amniocentesis once pregnancy is confirmed, as advised by your doctor.
Lupin Diagnostics PGT SR - 4 Embryos Test Price
The PGT SR - 4 Embryos Test cost at Lupin Diagnostics starts at ₹57,500. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 57500 |
| CHENNAI | 57500 |
| HYDERABAD | 57500 |
| KOLKATA | 57500 |
| NAVI MUMBAI | 57500 |
| PUNE | 57500 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your PGT SR - 4 Embryos Test online:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection and submission.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
PGT-SR is designed for couples where one partner carries a known structural chromosomal rearrangement, such as a translocation or inversion. PGT-A, by contrast, screens embryos for whole-chromosome abnormalities (aneuploidy) in couples without a known structural rearrangement. Both tests use NGS technology but address different genetic concerns.
Any couple where one or both partners have been confirmed to carry a balanced chromosomal rearrangement should discuss this test with their fertility specialist. It is particularly relevant for those who have experienced recurrent miscarriages, repeated IVF failures, or have a family history of chromosomal disorders.
Five to six trophectoderm cells are removed from the outer layer of the blastocyst. Since a healthy blastocyst contains over 100 cells, this represents a very small proportion of the total and does not affect the embryo's further development.
Lupin Diagnostics delivers results within 18 days from receipt of the samples. During this time, the embryos are kept frozen safely until the results are reviewed, and a transfer decision is made.
If all tested embryos show unbalanced chromosomal imbalances, your fertility specialist will discuss the available options with you. These may include undertaking another IVF cycle to generate additional embryos for testing, or exploring other family-building paths. A genetic counsellor can provide further guidance.
The biopsy removes cells only from the trophectoderm, the outer layer that develops into the placenta. The inner cell mass, which becomes the baby, is not touched. Because the embryo has over 100 cells at this stage, removing five to six has no meaningful effect on its development.
The test has a high level of diagnostic accuracy, but no genetic test is entirely without limitation. It cannot detect all chromosomal or genetic conditions. Confirmatory prenatal testing through CVS or amniocentesis is recommended once pregnancy is confirmed. Always discuss the benefits and limitations with your fertility specialist and genetic counsellor before proceeding.
PGT SR - 4 Embryos Test: Booking, Price, and Results
