PGT SR - 3 Embryos Test: Booking, Price, and Results
About PGT SR - 3 Embryos Test
| Field | Value |
|---|---|
| Also Known As | PGT-SR, Preimplantation Genetic Testing for Structural Rearrangements, PGT for Chromosomal Rearrangements |
| Sample Type | Embryo biopsy (Day 5 trophectoderm cells) |
| Fasting Required | No (not applicable; the test is performed on embryo cells, not on patient blood or urine) |
| Report Time | 18 days |
| Recommended For | Couples where one or both partners carry a balanced chromosomal rearrangement; couples with recurrent pregnancy loss or repeated implantation failure |
| Price | Starting at ₹43,500 |
What Is a PGT SR - 3 Embryos Test?
The PGT SR - 3 Embryos Test screens three embryos for chromosomal imbalances caused by a structural rearrangement carried by one or both parents. The full name is Preimplantation Genetic Testing for Structural Rearrangements, also referred to as PGT-SR. It is carried out as part of an in vitro fertilisation (IVF) cycle, before any embryo is transferred to the uterus. The test analyses a small number of cells taken by biopsy from the outer layer of each embryo.
What Does a PGT SR - 3 Embryos Test Measure?
The PGT SR - 3 Embryos Test procedure examines the chromosomal makeup of each biopsied embryo. It checks for imbalances arising from the parental rearrangement and also detects random chromosomal number errors. The following types of abnormalities are assessed:
| What Is Checked | Plain-Language Explanation |
|---|---|
| Unbalanced structural rearrangements | Extra or missing chromosome material inherited from a parent who carries a balanced rearrangement |
| Reciprocal translocations | Segments of genetic material that have swapped between two chromosomes |
| Robertsonian translocations | A specific type of translocation involving acrocentric chromosomes (13, 14, 15, 21, and 22) |
| Inversions | A chromosome segment that has flipped position within the same chromosome |
| Sporadic aneuploidies | Random gains or losses of whole chromosomes, unrelated to the parental rearrangement |
Why Is a PGT SR - 3 Embryos Test Done?
Couples who carry a balanced chromosomal rearrangement often face reproductive challenges. This test helps identify which embryos have a normal or balanced chromosome content, improving the chances of a successful pregnancy. Below are the key reasons a doctor may recommend it.
Common Symptoms That May Require This Test
The test is not triggered by physical symptoms in the usual sense. Instead, it is indicated based on reproductive history and genetic findings. A fertility specialist may recommend it when a couple has experienced:
- Two or more unexplained pregnancy losses (recurrent miscarriage)
- Repeated failure to achieve pregnancy after embryo transfer (implantation failure)
- A confirmed balanced chromosomal rearrangement in one or both partners on karyotype testing
- A previous pregnancy affected by an unbalanced chromosomal condition
- A family history of inherited chromosomal rearrangements
Conditions This Test Can Help Detect
The test is used to identify embryos affected by chromosomal imbalances. The following conditions in embryos are assessed:
- Unbalanced reciprocal translocation (extra or missing chromosome segments from a parental reciprocal translocation)
- Unbalanced Robertsonian translocation (chromosomal gains or losses from a Robertsonian rearrangement)
- Chromosomal inversions resulting in unbalanced embryos
- Sporadic aneuploidy (incorrect total chromosome number) detected alongside the targeted rearrangement screening
How to Prepare and What to Expect
The PGT SR - 3 Embryos Test is performed on embryo cells within an IVF programme, not directly on the patient. Preparation involves several clinical and administrative steps before the embryo biopsy can take place.
Do You Need to Fast?
No fasting is required. The test is performed on embryo biopsy cells, so dietary restrictions do not apply to this test.
Practical Tips Before Your Test
The following steps must be completed before the biopsy sample is collected and dispatched:
- Bring a detailed clinical history, including symptoms, previous pregnancy outcomes, and all past reports, as this is required for the test
- Submit a duly filled Test Request Form (TRF) and biopsy worksheet before sample dispatch
- Ensure a signed consent form is provided by both partners
- Both partners should have undergone karyotype analysis (a blood test to identify the specific chromosomal rearrangement) before the IVF cycle
- Attend all genetic counselling sessions required by your fertility centre before starting the cycle
Step-by-Step Procedure
- Eggs are fertilised using intracytoplasmic sperm injection (ICSI), the preferred fertilisation method for this type of testing.
- Fertilised eggs are cultured in the laboratory until they develop into blastocysts, typically by day 5, and sometimes by day 6.
- An embryologist takes a small biopsy of 5 to 6 cells from the trophectoderm, the outer layer of the blastocyst that later forms the placenta. The inner cells that develop into the baby are not disturbed.
- Biopsied embryos are frozen (cryopreserved) and stored safely while the genetic analysis is carried out.
- The biopsy sample is placed in the PGT collection kit and transported on dry ice to the laboratory for NGS (next-generation sequencing) analysis.
- The laboratory processes the sample through whole-genome amplification, library preparation, sequencing, and data analysis before generating a report.
Factors That Can Affect Accuracy
Several factors can influence the reliability of the test result:
- Cumulus cells attached to the embryo surface at the time of biopsy can interfere with the analysis
- Embryo quality and developmental stage at the time of biopsy
- Mosaicism, where different cells within the same embryo carry different chromosome content, can produce complex results
- Technical issues during sample handling or transport, such as insufficient DNA or chain-of-custody errors
- Laboratory expertise and the sequencing technology used
Understanding Your PGT SR - 3 Embryos Test Results
Results are reviewed jointly by your fertility specialist and a genetic counsellor. Each embryo receives an individual classification. The table below explains the possible result categories:
| Result Category | What It Means |
|---|---|
| Euploid or Balanced | Normal chromosome number or a balanced rearrangement. The embryo is generally considered suitable for transfer. |
| Aneuploid or Unbalanced | Abnormal chromosome content detected. This embryo is typically not recommended for transfer. |
| Mosaic | A mix of chromosomally normal and abnormal cells within the embryo. Requires detailed discussion with a genetic counsellor before any transfer decision. |
| No Result or Non-Informative | Insufficient DNA was obtained, or a technical issue occurred. A repeat biopsy may be discussed with your fertility team. |
Disclaimer: These categories are general guidelines. Your doctor will interpret your embryo results in the context of your full clinical picture, medical history, and reproductive goals. Always consult a qualified healthcare professional and a certified genetic counsellor for personalised medical advice.
Results During Special Conditions
The proportion of chromosomally normal embryos varies depending on the type of rearrangement and other factors:
- Female carriers of translocations tend to have a lower percentage of normal or balanced embryos than male carriers, regardless of translocation type.
- Increasing maternal age reduces the likelihood of obtaining a chromosomally normal embryo in any IVF cycle.
- In reciprocal translocation carriers, there is a meaningful chance that no chromosomally normal embryo may be available for transfer in a given cycle, even when several embryos are biopsied.
How to Maintain Healthy Outcomes
These are general wellness pointers for couples going through an IVF and PGT cycle:
- Follow all instructions from your fertility team regarding medication protocols, appointments, and lifestyle during the IVF cycle.
- Attend every genetic counselling appointment scheduled before and after testing to ensure results are properly understood.
- Discuss all result categories, including mosaic results, with your specialist before making any decisions about embryo transfer.
Lupin Diagnostics PGT SR - 3 Embryos Test Price
The PGT SR - 3 Embryos Test cost at Lupin Diagnostics starts at ₹43,500. This test requires a visit to a Lupin Diagnostics centre or an affiliated fertility facility; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 43500 |
| CHENNAI | 43500 |
| HYDERABAD | 43500 |
| KOLKATA | 43500 |
| NAVI MUMBAI | 43500 |
| PUNE | 43500 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The PGT SR - 3 Embryos Test online booking process is straightforward:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for coordination of sample collection with your fertility clinic.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
PGT-SR stands for Preimplantation Genetic Testing for Structural Rearrangements. It is recommended for couples where one or both partners have a confirmed balanced chromosomal rearrangement, such as a translocation or inversion. It is also advised for couples who have experienced repeated miscarriages or multiple failed embryo transfers.
PGT-A screens embryos for random chromosomal number errors that can arise in any IVF cycle. PGT-SR is a targeted test used when a specific, known chromosomal rearrangement is present in one or both parents. Both tests use similar laboratory technology but address different clinical questions.
Studies suggest that roughly 35% of blastocysts in couples carrying balanced rearrangements are chromosomally normal or balanced. This figure varies depending on the type of rearrangement, which partner carries it, and the maternal age.
At Lupin Diagnostics, the report delivery time for this test is 18 days from the receipt of the biopsy sample. You will receive your report digitally once the analysis is complete.
The biopsy removes cells only from the trophectoderm, the outer layer that later becomes the placenta. The inner cells that form the baby are not touched. A small proportion of embryos, around 5%, may be affected by handling during the biopsy process, which is why it is performed by experienced embryologists.
Your fertility specialist will review each embryo's result with you. Embryos classified as euploid or balanced are generally considered for transfer. Mosaic or unbalanced results require further discussion. Embryos that are not selected can remain frozen for a defined period, as agreed with your centre.
Yes. PGT-SR is highly informative but not 100% conclusive. Professional guidelines recommend that couples with a normal PGT-SR result are offered confirmatory prenatal testing, such as chorionic villus sampling (CVS) or amniocentesis, once pregnancy is established.
PGT SR - 3 Embryos Test: Booking, Price, and Results
