PGT SR - 2 Embryos Test: Booking, Price, and Results
About PGT SR - 2 Embryos Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | PGT-SR, Preimplantation Genetic Testing for Structural Rearrangements, PGD for chromosomal rearrangements |
| Sample Type | Embryo biopsy (trophectoderm cells from blastocyst-stage embryo, Day 5 or Day 6) |
| Fasting Required | Not applicable. This test is performed on embryo cells, not on a patient's blood or urine sample. |
| Report Time | 18 days |
| Recommended For | Couples where one or both partners carry a balanced chromosomal translocation, inversion, or other structural rearrangement; all genders undergoing IVF |
| Price | Starting at ₹28,500 |
What Is a PGT SR - 2 Embryos Test?
The PGT SR - 2 Embryos Test is a specialised genetic test performed on embryos created through IVF. It screens two embryos for chromosomal imbalances that arise when one or both parents carry a structural chromosomal rearrangement. The test is also known as Preimplantation Genetic Testing for Structural Rearrangements, or PGT-SR.
A small sample of cells is taken from the outer layer of each blastocyst-stage embryo. These cells are then analysed using Next-Generation Sequencing (NGS), a method that reads the genetic material in detail. This helps identify which embryos are suitable for transfer before pregnancy begins.
What Does a PGT SR - 2 Embryos Test Measure?
This test analyses the chromosomes of two embryo biopsy samples to detect genetic imbalances. The following are the key findings the test screens for:
| Finding | What It Means |
|---|---|
| Unbalanced chromosomal rearrangements | Extra or missing chromosomal material resulting from a parental structural rearrangement |
| Aneuploidies (abnormal chromosome number) | Embryos with too many or too few chromosomes, not related to the parental rearrangement |
| Chromosome copy number variations (CNVs) | Deletions or duplications of chromosomal segments resulting in an abnormal amount of genetic material |
Because general aneuploidy screening is included in the PGT-SR test procedure, a separate PGT-A test is not required for the same embryos.
Why Is a PGT SR - 2 Embryos Test Done?
Couples who carry balanced chromosomal rearrangements face a higher risk of miscarriage and chromosomally abnormal pregnancies. The PGT SR test helps identify embryos with the best chance of a healthy pregnancy before transfer.
Common Symptoms That May Require This Test
Doctors typically recommend the PGT SR - 2 Embryos Test when a couple presents with one or more of the following:
- Recurrent pregnancy loss (two or more miscarriages)
- A known balanced chromosomal translocation in one or both partners
- A known chromosomal inversion identified through parental karyotyping
- Repeated IVF implantation failure without a clear cause
- A previous pregnancy or a child affected by a chromosomal imbalance
- A family history of structural chromosomal rearrangements
Conditions This Test Can Help Detect
Testing embryos before transfer can help reduce the risk of the following outcomes:
- Unbalanced chromosomal rearrangements leading to miscarriage or pregnancy loss
- Implantation failure caused by chromosomally abnormal embryos
- Birth of a child with congenital anomalies due to chromosomal imbalance
- Passing a balanced rearrangement to the next generation
How to Prepare and What to Expect
The PGT SR - 2 Embryos test procedure takes place within an IVF cycle. The embryo biopsy is performed by trained embryologists in a specialised fertility laboratory.
Do You Need to Fast?
No fasting is required. This test is performed on embryo cells, not on any blood or urine sample from the patient. Standard IVF cycle instructions from your fertility specialist apply.
Practical Tips Before Your Test
The following documents and steps are required before the test can proceed:
- Bring a detailed clinical history, including symptoms, previous test results, and family history, as this is required for the test
- Carry a duly filled Test Request Form (TRF) and biopsy worksheet to the IVF centre
- Bring a signed consent form as instructed by your fertility clinic
- Provide parental karyotype results confirming the structural chromosomal rearrangement
- Attend genetic counselling before the IVF cycle begins, as recommended
- Disclose all current medications to your fertility specialist
Step-by-Step Procedure
- The patient completes the IVF cycle, including ovarian stimulation and egg retrieval, as directed by the fertility specialist.
- Fertilisation is performed using ICSI (intracytoplasmic sperm injection), the preferred method for PGT-SR cycles. Embryos are cultured in the laboratory until they reach the blastocyst stage, typically on Day 5 or Day 6.
- The embryologist uses a laser-assisted technique to open the outer shell of the blastocyst and remove 5 to 6 trophectoderm cells. This is the outer layer that later forms the placenta. The inner cell mass, which develops into the baby, is not disturbed.
- After biopsy, the two embryos are immediately vitrified (frozen) for safekeeping. The biopsied cell samples are placed in a PGT collection kit and shipped on dry ice to the Lupin Diagnostics laboratory.
- In the laboratory, the cells are analysed using NGS technology combined with validated bioinformatic methods to assess chromosomal structure and number.
- A detailed genetic report is prepared and sent to your doctor within 18 days of the laboratory receiving the samples.
Factors That Can Affect Accuracy
The following factors may influence test accuracy:
- Embryo quality and blastocyst grade at the time of biopsy
- Number of cells successfully obtained during the biopsy
- Mosaicism, where an embryo contains two or more genetically different cell populations
- Technical variables during whole-genome amplification and sequencing
- Insufficient DNA in the sample, which may lead to an inconclusive result
Understanding Your PGT SR - 2 Embryos Test Results
Results from the PGT SR - 2 Embryos Test are reported per embryo. Your fertility specialist and genetic counsellor will review the findings with you and recommend next steps based on the results.
| Result Category | Meaning | Implication |
|---|---|---|
| Normal (Euploid) | No chromosomal imbalance detected, and the embryo has not inherited the parental structural rearrangement | Generally considered suitable for transfer |
| Balanced Carrier | An embryo carries the parental balanced translocation or inversion, but has no net gain or loss of genetic material | May be suitable for transfer; genetic counselling may be recommended |
| Unbalanced / Abnormal | Extra or missing chromosomal material detected due to a structural rearrangement or other chromosomal abnormality | Generally not selected for transfer |
| Inconclusive / No Result | No reliable result obtained because of insufficient DNA or technical limitations | Repeat testing or re-biopsy may be considered |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Note: Most NGS-based technologies cannot distinguish between a normal embryo and one carrying a balanced rearrangement. Results are typically reported as balanced or unbalanced.
How to Maintain Healthy Levels
While chromosomal structure cannot be changed through lifestyle, the following general steps support the best possible outcome during an IVF cycle:
- Follow all dietary and activity guidance given by your fertility specialist during ovarian stimulation.
- Stay well hydrated and maintain a consistent sleep routine throughout the cycle.
- Attend all recommended genetic counselling sessions to understand your results and discuss embryo transfer options.
Lupin Diagnostics PGT SR - 2 Embryos Test Price
The PGT SR - 2 Embryos Test cost starts at ₹28,500 at Lupin Diagnostics. This test requires embryo biopsy, which must be performed in a specialised IVF laboratory. Home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 28500 |
| CHENNAI | 28500 |
| HYDERABAD | 28500 |
| KOLKATA | 28500 |
| NAVI MUMBAI | 28500 |
| PUNE | 28500 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The steps below explain how to arrange PGT SR - 2 Embryos test online booking with Lupin Diagnostics:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection coordination with your IVF clinic.
- Receive your report via email or WhatsApp within 18 days of the laboratory receiving the embryo biopsy samples.
Frequently Asked Questions
PGT-SR stands for Preimplantation Genetic Testing for Structural Rearrangements. It is recommended for couples where one or both partners carry a balanced chromosomal translocation, inversion, or complex rearrangement. These conditions increase the risk of miscarriage and chromosomally abnormal pregnancies.
A trained embryologist uses a laser-assisted technique to remove 5 to 6 cells from the outer layer of the blastocyst on Day 5 or Day 6 of embryo development. The part of the embryo that grows into the baby is not touched during this process.
No part of the biopsy procedure is performed on the patient directly. The cells are taken from the embryo in the laboratory. Patients do experience the standard IVF cycle, which includes injections and egg retrieval under sedation, but no additional procedure is done specifically for the biopsy.
The report is delivered within 18 days from the date the laboratory receives the embryo biopsy samples. Your fertility specialist will contact you once the results are available and arrange a consultation to discuss the findings.
No. PGT-SR can detect chromosomal imbalances arising from structural rearrangements and numerical abnormalities. It cannot detect balanced rearrangements where no chromosomal material is gained or lost. It also cannot detect single-gene disorders such as cystic fibrosis or sickle cell anaemia. Confirmatory prenatal testing is recommended during pregnancy.
If no suitable embryo is identified, the fertility team and genetic counsellor will discuss further options. These may include undergoing another IVF cycle, exploring donor gametes, or other family planning choices.
No. The embryo biopsy must be performed by trained embryologists in a specialised IVF laboratory. The sample cannot be collected at home. Please contact Lupin Diagnostics or your IVF centre to coordinate sample dispatch arrangements.
PGT SR - 2 Embryos Test: Booking, Price, and Results
