PGT M - 2 Variants - 5 Embryos Test: Booking, Price, and Results
About PGT M - 2 Variants - 5 Embryos Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | PGT-M (Preimplantation Genetic Testing for Monogenic Disorders), Preimplantation Genetic Diagnosis (PGD), Single Gene Testing, Monogenic PGT |
| Sample Type | Embryo biopsy (trophectoderm cells from blastocyst-stage embryos, Day 5 or Day 6) |
| Fasting Required | No. This test is performed on embryo cells, not on a blood or urine sample from the patient. |
| Report Time | 24 days after embryo biopsy. A pre-test design phase (3 to 12 weeks) is required before the IVF cycle begins. |
| Recommended For | Couples undergoing IVF who are known carriers of a single-gene (monogenic) disorder or have a family history of an inherited genetic condition |
| Price | Starting at ₹1,45,000 |
What Is a PGT M - 2 Variants - 5 Embryos Test?
The PGT M - 2 Variants - 5 Embryos test is a specialised genetic test performed on embryos created through in vitro fertilisation (IVF). It screens five embryos for two specific disease-causing genetic variants (mutations) known to be present in a family. The test helps identify which embryos do not carry the condition so that only those embryos are selected for transfer. It is also referred to as PGT-M or Preimplantation Genetic Testing for Monogenic Disorders.
What Does a PGT M - 2 Variants - 5 Embryos Test Measure?
The PGT M - 2 Variants - 5 Embryos test procedure analyses the genetic material in cells biopsied from each embryo. It uses Next Generation Sequencing (NGS), an advanced DNA-reading technology, to examine two specific mutations. Here is what the analysis covers:
| What Is Analysed | What It Tells You |
|---|---|
| Presence or absence of specific genetic variants | Whether each embryo has inherited the disease-causing mutation(s) |
| Haplotype linkage analysis | Tracks the inheritance pattern of the mutation through the family to improve accuracy |
| Embryo genetic classification | Labels each embryo as affected, carrier or unaffected |
For the PGT M - 2 Variants - 5 Embryos test, two specific mutations are tracked across five embryos, giving the fertility team clear data on which embryos are suitable for transfer.
Why Is a PGT M - 2 Variants - 5 Embryos Test Done?
This test is ordered for couples who know they carry a genetic condition and want to reduce the risk of passing it to their child. This test is ordered as part of an IVF cycle to screen embryos before transfer.
Common Symptoms That May Require This Test
This test is not triggered by symptoms. Instead, it is recommended based on genetic risk factors. The following situations commonly lead a doctor to recommend the PGT M - 2 Variants - 5 Embryos test:
- Known carrier status for an inherited single-gene disorder in one or both partners
- A previous pregnancy or child affected by a genetic condition
- A strong family history of an inherited disease such as thalassaemia or haemophilia
- A relative recently diagnosed with a hereditary condition prompting family screening
- Recurrent pregnancy loss associated with a genetic cause
- Belonging to a population group at higher risk for specific conditions, such as beta-thalassaemia carriers in certain regions of India
- A recommendation from a genetic counsellor following carrier screening
Conditions This Test Can Help Detect
The PGT M - 2 Variants - 5 Embryos test is typically sought by couples with a family history of the following conditions:
- Beta-thalassaemia and sickle cell anaemia
- Cystic fibrosis
- Haemophilia A and B
- Duchenne muscular dystrophy
- Huntington's disease
- Spinal muscular atrophy
- Fragile X syndrome
- Hereditary cancer syndromes (autosomal dominant forms)
- Rare metabolic disorders such as phenylketonuria and methylmalonic acidaemia
How to Prepare and What to Expect
This is not a standard blood test. The PGT M - 2 Variants - 5 Embryos test procedure is performed on embryo cells during an IVF cycle. It involves several stages, beginning weeks before the IVF cycle itself.
Do You Need to Fast?
No fasting is required. This test is performed on embryo cells biopsied in an IVF laboratory, not on a sample collected from the patient directly.
Practical Tips Before Your Test
The following steps are essential before the embryo biopsy and genetic analysis can take place:
- Bring a detailed clinical history, including your symptoms, previous test results and family history, as this is required for the test.
- Submit a duly completed Test Request Form (TRF), biopsy worksheet and signed consent form as instructed by your clinic.
- Provide DNA samples from both partners, and potentially from affected family members, to allow the laboratory to design a test specific to your family's mutations.
- Attend a genetic counselling session before starting the IVF cycle.
- Allow 3 to 12 weeks for the pre-test design phase before your IVF cycle begins.
- Discuss all medications you are taking with your fertility specialist, as they will guide you through the IVF preparation protocol.
Step-by-Step Procedure
The PGT M - 2 Variants - 5 Embryos test procedure takes place within a full IVF cycle. Here is what the process looks like:
- Pre-test design: The laboratory designs a custom genetic test for your specific mutations using DNA from both partners and family members. This phase takes 3 to 12 weeks.
- IVF cycle: The woman undergoes ovarian stimulation and egg retrieval. Eggs are fertilised using ICSI (intracytoplasmic sperm injection, where a single sperm is injected directly into each egg).
- Embryo culture: Fertilised embryos are grown in the laboratory until they reach the blastocyst stage, typically on day 5 or day 6, when each embryo contains approximately 100 cells.
- Trophectoderm biopsy: A trained embryologist removes 5 to 8 cells from the outer layer of each blastocyst (the trophectoderm, which later forms the placenta). This biopsy removes only about 5 to 8% of the embryo's total cells.
- Vitrification: Each embryo is immediately frozen after biopsy while the genetic analysis is carried out.
- Genetic analysis and report: The biopsied cells are sent to the Lupin Diagnostics laboratory, where NGS technology analyses each embryo for the two specified variants. Embryos are classified as affected, carrier or unaffected, and your fertility team uses this information to select suitable embryos for transfer.
Factors That Can Affect Accuracy
Several factors can influence the reliability of the result:
- Quality and cellular integrity of the biopsy sample
- Availability of DNA samples from relevant family members for accurate test design
- The stage of embryo development at the time of biopsy
- Rare technical events such as allele dropout (when one copy of a gene is not detected), though NGS technology significantly reduces this risk
- Embryo quality and overall health at the time of biopsy
Understanding Your PGT M - 2 Variants - 5 Embryos Test Results
Results from the PGT M - 2 Variants - 5 Embryos test classify each of the five embryos into one of the categories below. Your genetic counsellor and fertility specialist will interpret the results in the context of your specific condition and family history.
| Result Category | Meaning | Typical Recommendation |
|---|---|---|
| Unaffected | No copies of the disease-causing mutation detected | Recommended for embryo transfer |
| Carrier | One copy of the mutation present; embryo will not be affected | May be considered for transfer depending on condition and counselling |
| Affected | Carries the disease-causing mutation (both copies for recessive, one copy for dominant conditions) | Not recommended for transfer |
| Inconclusive | Technical issue with the sample | Re-biopsy or further testing may be required |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history and other factors. Always consult a qualified healthcare professional for personalised medical advice.
How to Maintain Healthy Levels
This test analyses embryo genetics and does not measure levels that can be modified by lifestyle. However, the following general steps support the best possible outcomes during IVF:
- Maintain a balanced diet and a healthy weight, as this supports ovarian response and embryo quality
- Avoid smoking and alcohol during the IVF preparation period, as both can affect egg and sperm quality
- Attend all recommended follow-up appointments with your fertility specialist and genetic counsellor
- Discuss your embryo transfer options with your fertility specialist based on the full picture, including embryo quality and your reproductive history
- Confirmatory prenatal testing such as amniocentesis is generally recommended after embryo transfer to verify results during pregnancy
Lupin Diagnostics PGT M - 2 Variants - 5 Embryos Test Price
The PGT M - 2 Variants - 5 Embryos test cost starts at ₹1,45,000. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test, as embryo biopsy must be performed in a specialised IVF laboratory.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 145000 |
| CHENNAI | 145000 |
| HYDERABAD | 145000 |
| KOLKATA | 145000 |
| NAVI MUMBAI | 145000 |
| PUNE | 145000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The PGT M - 2 Variants - 5 Embryos test online booking process is straightforward:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for coordination of sample collection with your IVF clinic and submission of required documentation.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
PGT-M screens embryos for a specific inherited single-gene disorder, such as thalassaemia or cystic fibrosis, that is already known to run in your family. PGT-A, by contrast, checks for an abnormal number of chromosomes in the embryo. The two tests serve different purposes and may sometimes be performed together.
This means the laboratory will screen five embryos for two distinct disease-causing genetic mutations identified in your family. The number of variants and embryos determines the scope and complexity of the analysis, which is why both are specified in the test name.
The pre-test design phase, where the laboratory builds a custom genetic test for your family's specific mutations, takes 3 to 12 weeks. After the embryo biopsy is performed during your IVF cycle, results are delivered within 24 days. Your fertility clinic will help you plan your IVF timeline accordingly.
NGS technology delivers very high accuracy, but no genetic test is 100% accurate. Confirmatory prenatal testing, such as amniocentesis at around 16 weeks of pregnancy, is usually recommended after embryo transfer to verify the result.
No. The PGT M - 2 Variants - 5 Embryos test home collection option is not available. The embryo biopsy must be performed in a specialised IVF laboratory by a trained embryologist. Patients need to attend a fertility centre for the IVF cycle and biopsy procedure.
If all five embryos carry the disease-causing mutation, your fertility specialist and genetic counsellor will discuss the available options with you. These may include a repeat IVF cycle to create additional embryos for testing or other family-building approaches suited to your situation.
Yes. Even after a successful embryo transfer using an embryo classified as unaffected by PGT-M, confirmatory prenatal diagnosis during pregnancy is generally recommended. Your doctor will advise on the appropriate form of prenatal testing based on your specific condition.
PGT M - 2 Variants - 5 Embryos Test: Booking, Price, and Results
