PGT M - 1 Variant - 2 Embryos Test: Booking, Price, and Results
About PGT M - 1 Variant - 2 Embryos Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | PGT-M, Preimplantation Genetic Testing for Monogenic Disorders, Single Gene PGT, PGD (older term) |
| Sample Type | Embryo biopsy (trophectoderm cells from a day 5 or day 6 blastocyst); blood or saliva samples from parents and relevant family members for test setup |
| Fasting Required | Not applicable |
| Report Time | 24 days |
| Recommended For | Couples undergoing IVF who carry or are affected by a known single-gene disorder |
| Price | Starting at ₹28,800 |
What Is a PGT M - 1 Variant - 2 Embryos Test?
The PGT M - 1 Variant - 2 Embryos Test is a genetic test performed on embryos created through IVF (in vitro fertilisation). It checks whether embryos carry a specific disease-causing gene mutation known to run in the family. This particular package covers one genetic variant across two embryos. The test is also known as PGT-M (Preimplantation Genetic Testing for Monogenic Disorders) or, by its older name, Preimplantation Genetic Diagnosis (PGD). A small number of cells are removed from each embryo and analysed in a laboratory before any embryo is transferred to the womb.
What Does a PGT M - 1 Variant - 2 Embryos Test Measure?
This test examines each embryo's genetic material to determine whether it has inherited the specific mutation that causes a known hereditary condition in the family. The results classify each embryo into one of the following categories.
| Result Category | What It Means |
|---|---|
| Unaffected | The embryo does not carry the disease-causing variant being tested and is generally considered suitable for transfer |
| Carrier | An embryo carries one copy of a recessive disease-causing variant but is not expected to develop the condition; generally considered for transfer following counselling |
| Affected | The embryo has inherited the disease-associated variant(s) in a pattern predicted to cause the condition being tested and is generally not selected for transfer |
| Inconclusive | Genetic analysis could not provide a reliable result due to technical or biological limitations; repeat testing, re-biopsy, or further specialist review may be considered |
The test uses Next-Generation Sequencing (NGS), a method that reads large sections of genetic material quickly and with high accuracy. Studies report that PGT-M identifies affected and unaffected embryos with greater than 99% accuracy.
Why Is a PGT M - 1 Variant - 2 Embryos Test Done?
A doctor may recommend this test when a couple has a known hereditary condition and wishes to avoid passing it on to their child. Below are the common reasons it is prescribed.
Common Symptoms That May Require This Test
The following indications are typically discussed during genetic counselling before an IVF cycle:
- A known hereditary single-gene disorder in one or both partners
- A previous child born with a genetic condition
- Confirmed carrier status identified during routine genetic screening
- Recurrent pregnancy loss linked to a genetic cause
- A consanguineous (blood-related) marriage between partners
- Family history of conditions such as beta thalassaemia, haemophilia, or muscular dystrophy
Conditions This Test Can Help Detect
The PGT M - 1 Variant - 2 Embryos Test procedure can be customised for nearly any single-gene disorder once the specific family mutation has been identified. Conditions commonly tested include:
- Beta thalassaemia and sickle cell anaemia
- Cystic fibrosis
- Haemophilia A and B
- Duchenne muscular dystrophy
- Huntington's disease
- Fragile X syndrome
- Myotonic dystrophy type 1
- Spinal muscular atrophy
- Hereditary cancer syndromes (e.g., BRCA-related)
- Lysosomal storage disorders and other rarer inherited conditions
How to Prepare and What to Expect
The PGT M - 1 Variant - 2 Embryos Test is not a standard blood test. It is performed during an IVF cycle and requires careful preparation in advance.
Do You Need to Fast?
Fasting is not required for this test. The embryo biopsy is performed in an IVF laboratory and does not involve dietary restrictions.
Practical Tips Before Your Test
The following steps will help ensure the process runs smoothly:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Carry all prior genetic testing reports and share them with the fertility centre before your consultation
- Ensure a completed Test Request Form (TRF), biopsy worksheet, and signed consent form are submitted to the laboratory
- Attend a genetic counselling session before starting the IVF cycle, as the test setup can take several weeks
- Confirm that relevant family members (parents, siblings, or an affected child) are available to provide blood or saliva samples for haplotype analysis during setup
Step-by-Step Procedure
- The female partner undergoes ovarian stimulation followed by egg retrieval; the eggs are fertilised using ICSI (intracytoplasmic sperm injection, where a single sperm is injected directly into each egg).
- Fertilised eggs are cultured in the laboratory until they develop into blastocysts, typically by day 5 or day 6.
- A trained embryologist performs a trophectoderm biopsy: a small opening is made in the outer shell of each blastocyst using a laser, and 5 to 8 cells are carefully removed for genetic analysis.
- The biopsied cells are sent to the laboratory for NGS-based genetic analysis; the embryos are vitrified (rapidly frozen) while awaiting results.
- Results are reported within 24 days; your fertility specialist will then discuss which embryos are suitable for transfer.
Factors That Can Affect Accuracy
Several factors may influence the reliability of results:
- Quality of the embryo biopsy sample
- Embryo mosaicism (when an embryo contains a mix of genetically normal and abnormal cells)
- Allele dropout (when one copy of a gene fails to amplify during analysis)
- Completeness of the family genetic workup and availability of suitable reference family members
- Complexity of the specific genetic variant being tested
Understanding Your PGT M - 1 Variant - 2 Embryos Test Results
Results classify each embryo as unaffected, carrier, or affected based on the specific mutation under study. Only a qualified fertility specialist or genetic counsellor can interpret these results in the context of your full medical and family history.
| Result | Interpretation |
|---|---|
| Unaffected | The embryo does not carry the disease-causing mutation; suitable for transfer |
| Carrier | An embryo carries one copy of the mutation (recessive conditions); generally suitable for transfer |
| Affected | The embryo carries mutation(s) that would cause disease; transfer is not recommended |
| Inconclusive | Result could not be determined; specialist advice required |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Embryo mosaicism can occasionally affect results. A mosaic embryo contains some cells with the mutation and some without, which may make classification less straightforward. Your specialist will guide you on the next steps if mosaicism is detected.
In addition, certain test setups using haplotype analysis may not be possible if a suitable reference family member is unavailable, for example, in cases of a new (de novo) mutation with no prior family history of the condition.
How to Maintain Healthy Levels
These are general guidance points to support informed decision-making during and after the process:
- Attend all recommended genetic counselling sessions before and after receiving results.
- Ask your specialist about combining the PGT M test with PGT-A (aneuploidy screening) for a broader assessment of embryo health when clinically appropriate.
- Once pregnancy is achieved, confirmatory prenatal testing such as amniocentesis or chorionic villus sampling may be advised to reconfirm results.
Lupin Diagnostics PGT M - 1 Variant - 2 Embryos Test Price
The PGT M - 1 Variant - 2 Embryos Test cost starts at ₹28,800 at Lupin Diagnostics. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 28800 |
| CHENNAI | 28800 |
| HYDERABAD | 28800 |
| KOLKATA | 28800 |
| NAVI MUMBAI | 28800 |
| PUNE | 28800 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection and submission.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
This means the test is designed to detect one specific genetic mutation known to cause disease in your family, and the package includes testing of up to 2 embryos created during your IVF cycle. If you have more embryos to test, separate packages covering 3, 4, or 5 embryos are also available.
This test is recommended for couples undergoing IVF who carry or are affected by a confirmed single-gene disorder and want to reduce the risk of passing it on to their child. It is also considered for couples with recurrent pregnancy loss linked to a genetic cause, or those in a consanguineous marriage.
PGT-M identifies affected and unaffected embryos with greater than 99% accuracy. However, no test is entirely without limitation, and confirmatory prenatal testing is still recommended after pregnancy is achieved to reconfirm results.
The full process has two phases. Test setup using parental and family samples can take several weeks. Once the embryo biopsy samples are submitted, the report is delivered within 24 days as per the Lupin Diagnostics schedule.
If both embryos carry the disease-causing mutation, your fertility specialist will discuss the available options with you. These may include undergoing another IVF cycle to create additional embryos for testing or exploring other family-building paths.
The PGT M - 1 Variant - 2 Embryos Test tests only for the one specific genetic condition known in your family. It does not screen for all possible genetic or developmental issues. While it significantly reduces the risk of the condition being inherited, it cannot guarantee an entirely healthy pregnancy or baby.
No. The PGT M - 1 Variant - 2 Embryos Test home collection option is not available. The embryo biopsy must be performed by a trained embryologist in a specialised IVF laboratory. You will need to visit a Lupin Diagnostics centre or an affiliated facility for sample submission.
PGT M - 1 Variant - 2 Embryos Test: Booking, Price, and Results
