PGT-A (9 Embryos) Test: Booking, Price, and Results

The PGT-A test uses Next-Generation Sequencing (NGS) technology to analyse all 23 chromosome pairs in each embryo. Here is what the test evaluates:

Because this test is performed on embryo cells rather than a patient's blood or urine sample, preparation is different from a standard diagnostic test. Below is everything you need to know before proceeding.

Do You Need to Fast?

No fasting is required. The PGT-A test procedure is carried out on embryo biopsy cells in the laboratory. There is no dietary restriction for the patient.

Practical Tips Before Your Test

Here are some practical steps to take before your embryos are tested:

• Complete a full IVF cycle, including ovarian stimulation and egg retrieval, before PGT-A can be arranged
• Attend genetic counselling before the test to understand what the results can and cannot tell you
• Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
• Ensure you carry the duly completed Test Request Form (TRF), biopsy worksheet, and signed consent form, as these are mandatory for processing
• Be prepared for your embryos to be frozen (vitrified) immediately after biopsy, while results are awaited
• Confirm that your IVF centre has trained embryologists and the necessary equipment for trophectoderm biopsy

Step-by-Step Procedure

The PGT-A test procedure involves several stages carried out by your IVF team and the genetics laboratory:

1. IVF cycle completion: Eggs are retrieved, fertilised in the laboratory, and cultured for five to six days until they reach the blastocyst stage.
2. Embryo biopsy: A trained embryologist uses a laser to open a small gap in the embryo's outer shell and removes five to eight cells from the trophectoderm (the outer layer that would form the placenta). The inner cell mass, which develops into the baby, is left undisturbed.
3. Embryo freezing: All biopsied embryos are immediately vitrified (frozen) and stored safely while testing is underway.
4. Sample dispatch: Biopsy cells are placed in the PGT Collection Kit and transported on dry ice to the Lupin Diagnostics genetics laboratory.
5. NGS analysis: The laboratory uses Next-Generation Sequencing to examine all 23 chromosome pairs in each embryo sample.
6. Results and next steps: Once results are ready, your fertility specialist reviews the findings and identifies euploid embryos suitable for frozen embryo transfer.

Factors That Can Affect Accuracy

The following factors may influence the reliability of PGT-A test results:

• The skill and experience of the embryologist performing the biopsy
• The developmental stage and quality of each embryo at the time of biopsy
• Natural embryonic mosaicism (some embryos contain a mix of normal and abnormal cells)
• Laboratory sequencing conditions and analytical methods
• The fact that biopsy samples come only from the trophectoderm, which may not always fully represent the inner cell mass

While chromosomal status cannot be changed, there are general steps that support a positive IVF outcome:

• Discuss all results, including mosaic findings, thoroughly with your fertility specialist and genetic counsellor before deciding on embryo transfer
• Continue with standard prenatal screening after a successful transfer, as PGT-A does not replace routine pregnancy testing
• Maintain general reproductive health through balanced nutrition, adequate rest, and regular communication with your healthcare team

Here is how to book your PGT-A test online with Lupin Diagnostics:

1. Select the PGT-A (9 Embryos) test on the Lupin Diagnostics website.
2. Choose your city and preferred centre location.
3. Visit the centre at your scheduled time, bringing your completed TRF, biopsy worksheet, signed consent form, and clinical history.
4. Receive your report via email or WhatsApp within the stipulated turnaround time.