PGT - A (2 Embryos) Test: Booking, Price, and Results
About PGT - A (2 Embryos) Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | PGT-A, Preimplantation Genetic Screening (PGS), Chromosome Screening, Aneuploidy Testing, Embryo Genetic Testing |
| Sample Type | Embryo biopsy (trophectoderm cells from a Day 5/6 blastocyst) |
| Fasting Required | Not applicable; the procedure is performed on embryos in the laboratory, not on the patient |
| Report Time | 18 days |
| Recommended For | Couples undergoing IVF; particularly women over 35, those with recurrent miscarriage, repeated IVF failure, or known chromosomal concerns |
| Price | Starting at ₹22,500 |
What is a PGT - A (2 Embryos) Test?
The PGT - A test — short for Preimplantation Genetic Testing for Aneuploidy — is a specialised genetic test performed on embryos created through IVF (in vitro fertilisation). It checks whether embryos have the correct number of chromosomes before being transferred to the uterus. The test is carried out on a small biopsy taken from each embryo and is also known as PGT-A, Preimplantation Genetic Screening (PGS), or aneuploidy testing. This version of the PGT - A (2 embryos) test covers chromosome screening for two embryos in a single IVF cycle.
What Does a PGT - A (2 Embryos) Test Measure?
The PGT - A test procedure uses Next-Generation Sequencing (NGS) to analyse the chromosomal makeup of each embryo. All 23 chromosome pairs are examined to determine whether the embryo is chromosomally normal.
The following aspects are assessed for each embryo:
| What is Assessed | What it Means |
|---|---|
| All 23 chromosome pairs | Checks whether every chromosome pair is present in the correct number |
| Euploidy status | Confirms whether the embryo has the normal complement of 46 chromosomes |
| Aneuploidy | Detects extra or missing whole chromosomes (abnormal) |
| Mosaicism | Identifies embryos where some cells are normal, and others are abnormal |
| Segmental abnormalities | Finds missing or extra portions of chromosomes rather than entire chromosomes |
Why is a PGT - A (2 Embryos) Test Done?
The PGT - A test is recommended during IVF cycles to help select embryos with the best chance of resulting in a healthy pregnancy. Below are the main reasons a fertility specialist may recommend it.
Common Symptoms That May Require This Test
Doctors typically recommend the PGT - A test based on clinical history rather than physical symptoms. The following are common indications:
- Advanced maternal age (over 35 years)
- Recurrent miscarriage with no identified cause
- Repeated IVF implantation failure
- Previous pregnancy affected by a chromosomal abnormality
- Family history of chromosomal conditions
- Desire to maximise the chance of a successful first embryo transfer
Conditions This Test Can Help Detect
The PGT - A test can help identify embryos carrying chromosomal abnormalities associated with the following:
- Down syndrome (Trisomy 21), Edwards syndrome (Trisomy 18), and Turner syndrome
- Chromosomal aneuploidies that cause implantation failure or early pregnancy loss
- Embryonic chromosomal abnormalities, which are responsible for 50 to 70% of early pregnancy losses
How to Prepare and What to Expect
The PGT - A test procedure is performed on embryos in an IVF laboratory. The patient does not undergo a separate collection procedure. Here is what couples need to know before and during the process.
Do You Need to Fast?
No fasting is required. This test is performed on embryo biopsy samples in the laboratory, not directly on the patient.
Practical Tips Before Your Test
The following steps help ensure the process runs smoothly:
- Complete a full IVF or ICSI cycle to create embryos before the test can be performed
- ICSI (intracytoplasmic sperm injection, where a single sperm is injected directly into an egg) is preferred over standard IVF fertilisation for PGT-A, as it reduces the risk of contamination
- Embryos must be cultured to the blastocyst stage (Day 5 or Day 6 after fertilisation) before biopsy
- Bring a detailed clinical history, including symptoms, previous test results, and family history, as this is required for the test
- Bring a duly filled Test Request Form (TRF), biopsy worksheet, and signed consent form to the centre
- Genetic counselling before and after testing is strongly advisable to help understand and act on results
Step-by-Step Procedure
The PGT - A test involves the following steps, carried out by embryologists and genetic specialists:
- Eggs are retrieved from the female partner and fertilised with sperm in the IVF laboratory.
- The resulting embryos are cultured in the laboratory until they reach the blastocyst stage, approximately 5 to 6 days after egg retrieval.
- The embryologist makes a small laser-assisted opening in the outer shell of the blastocyst, then gently removes 5 to 8 trophectoderm cells (the outer layer that would go on to form the placenta).
- Immediately after biopsy, the embryos are frozen using ultra-rapid vitrification and stored safely while testing is underway.
- The biopsied cells are sent to the genetics laboratory, where NGS (Next-Generation Sequencing) is used to analyse all 23 chromosome pairs.
- Results are reported within 18 days; the fertility team then discusses findings with the couple to plan the next steps.
Factors That Can Affect Accuracy
The following factors may influence the reliability of results:
- The skill of the embryologist performing the biopsy
- The number of cells successfully biopsied
- Laboratory quality and sequencing technology
- Mosaicism in the embryo, where a mix of normal and abnormal cells may not be fully representative of the whole embryo
- Technical factors such as amplification variation, contamination, or statistical test noise
Understanding Your PGT - A (2 Embryos) Test Results
Results of the PGT - A test are reported as one of four classifications. Your fertility specialist will use these results alongside your full clinical picture to guide treatment decisions.
| Result | Definition | What it May Indicate |
|---|---|---|
| Euploid (Normal) | 46 chromosomes detected; less than 30% of chromosome material is abnormal | Highest suitability for transfer; approximately 60% chance of live birth |
| Aneuploid (Abnormal) | Incorrect number of chromosomes; more than 70% of the chromosome material is abnormal | Not recommended for transfer; very low live birth rate of 0 to 1% |
| Mosaic | Between 30 and 70% of cells are chromosomally abnormal | May be considered for transfer in select cases after detailed genetic counselling |
| No Result / Inconclusive | The biopsy did not yield interpretable data | Does not indicate an abnormal embryo; re-biopsy may be possible |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Maternal age has a significant effect on aneuploidy rates. In women under 30, roughly 28% of embryos are aneuploid. This figure rises steadily with age and reaches approximately 67% in women over 40. Even in young, healthy couples, 30 to 50% of IVF embryos may be chromosomally abnormal. Technical factors such as amplification variation and contamination can occasionally produce inconclusive results.
How to Maintain Healthy Levels
While chromosomal status in embryos is not influenced by lifestyle, the following general tips support the overall IVF process:
- Follow your fertility specialist's guidance on embryo selection and transfer timing
- Maintain a balanced diet, adequate sleep, and manageable stress levels during IVF treatment
- Standard prenatal screening is still recommended during pregnancy, even after transferring a euploid embryo
Lupin Diagnostics PGT - A (2 Embryos) Test Price
The PGT - A test cost at Lupin Diagnostics starts at ₹22,500 for two embryos. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test, as the sample is an embryo biopsy collected in a specialised IVF laboratory setting.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 22500 |
| CHENNAI | 22500 |
| HYDERABAD | 22500 |
| KOLKATA | 22500 |
| NAVI MUMBAI | 22500 |
| PUNE | 22500 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The following steps outline how to book your PGT - A test online or at a centre:
- Select the PGT-A (2 Embryos) test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time; the embryo biopsy sample will be collected and dispatched from the IVF laboratory in the designated PGT collection kit on dry ice.
- Receive your report via email or WhatsApp within 18 days.
Frequently Asked Questions
The PGT - A test checks whether embryos created during IVF have the correct number of chromosomes (46). It helps identify which embryos are most likely to result in a healthy pregnancy and reduces the risk of miscarriage caused by chromosomal abnormalities.
The PGT - A test is primarily recommended for women over 35, couples with unexplained recurrent miscarriage, repeated IVF failure, a prior pregnancy with a chromosomal condition, or a family history of chromosomal issues. It may also benefit couples who wish to maximise the chances of a successful first transfer.
Yes. The biopsy removes approximately 5 to 8 cells from the outer layer of the blastocyst — the part that would form the placenta, not the baby itself. Research indicates that embryo biopsy does not harm the developing embryo or the baby that results from it.
At Lupin Diagnostics, results for the PGT - A (2 embryos) test are delivered within 18 days. During this time, the biopsied embryos are frozen and stored safely at the IVF centre.
Receiving no euploid results can be difficult, but it is not uncommon, particularly for older patients. Your fertility specialist will discuss options such as a further IVF cycle, using donor eggs, or evaluating whether mosaic embryos may be suitable for transfer in your specific situation.
No. The PGT - A test is a screening test, not a diagnostic test. Selecting a chromosomally normal embryo significantly improves the likelihood of a successful pregnancy, but standard prenatal testing during pregnancy is still advised even after a euploid embryo transfer.
The PGT - A test price at Lupin Diagnostics starts at ₹22,500 for two embryos. Costs across India generally range from ₹15,000 to ₹20,000 per embryo at other providers, with complete IVF cycles including PGT-A typically ranging from ₹2.5 lakh to ₹4 lakh in major cities. Please confirm current pricing directly with Lupin Diagnostics at the time of booking.
PGT - A (2 Embryos) Test: Booking, Price, and Results
