PGT-A (10 Embryos) Test: Booking, Price, and Results
About PGT-A (10 Embryos) Test
| Field | Value |
|---|---|
| Also Known As | PGT-A, Preimplantation Genetic Screening (PGS), Preimplantation Genetic Testing for Aneuploidies, Embryo Genetic Testing, Chromosome Screening |
| Sample Type | Trophectoderm biopsy (5 to 6 cells from a Day 5 blastocyst embryo) |
| Fasting Required | No — this test is performed on embryos during an IVF cycle, not on the patient directly |
| Report Time | 18 days |
| Recommended For | Couples undergoing IVF; women aged 35 and above; individuals with recurrent miscarriage; those with repeated IVF failures; couples with known chromosomal abnormalities |
| Price | Starting at ₹1,00,000 |
What is a PGT-A (10 Embryos) Test?
The PGT-A test — short for Preimplantation Genetic Testing for Aneuploidy — is a specialised genetic test performed during an IVF cycle. It analyses embryos for chromosomal abnormalities before they are transferred to the uterus. Also known as Preimplantation Genetic Screening (PGS), the test examines up to 10 embryos in this panel. The sample used is a small cluster of cells biopsied from each blastocyst embryo, not a blood or urine sample from the patient.
What Does a PGT-A (10 Embryos) Test Measure?
The PGT-A test procedure uses Next-Generation Sequencing (NGS) to examine the genetic material from biopsied embryo cells. Here is what the test evaluates:
| Parameter | What it Checks |
|---|---|
| Chromosomal ploidy status | Whether each embryo has the correct number of chromosomes (46 total) |
| All 23 chromosome pairs | All 22 autosomes plus the sex chromosomes X and Y |
| Mosaicism | Whether an embryo contains a mix of normal and abnormal cells |
| Segmental aneuploidies | Whether portions of chromosomes — rather than whole chromosomes — are missing or duplicated |
Why is a PGT-A (10 Embryos) Test Done?
A fertility specialist may recommend the PGT-A test for several reasons related to reproductive history and age.
Common Symptoms That May Require This Test
The following clinical situations are the most common reasons a doctor recommends this test:
- Advanced maternal age (35 years and above)
- Recurrent pregnancy loss or unexplained miscarriages
- Repeated IVF failure despite good embryo quality
- Known chromosomal abnormality in either partner
- Previously affected pregnancy with a chromosomal condition
- Unexplained implantation failure
Conditions This Test Can Help Detect
The test helps identify embryos affected by the following chromosomal conditions:
- Down syndrome (Trisomy 21)
- Edwards syndrome (Trisomy 18)
- Patau syndrome (Trisomy 13)
- Turner syndrome and other sex chromosome abnormalities
- Aneuploidy — gain or loss of one or more chromosomes from the normal count of 46
- Segmental aneuploidies — where a fragment of a chromosome is gained or lost
How to Prepare and What to Expect
The PGT-A test online booking and preparation process involves coordination between the couple, the IVF clinic, and the diagnostic laboratory.
Do You Need to Fast?
No fasting is required. The test is carried out on embryo biopsy samples, not on blood or any sample collected directly from the patient.
Practical Tips Before Your Test
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Carry the duly filled Test Request Form (TRF), biopsy worksheet, and signed consent form, as these are mandatory for processing
- Discuss your age, medical history, and prior pregnancy outcomes with your fertility specialist to confirm the test is appropriate for you
- Plan for genetic counselling before and after the test to help you understand the results fully
- Be aware that embryos are frozen immediately after biopsy; a frozen embryo transfer (FET) cycle will follow once results are ready
Step-by-Step Procedure
The PGT-A test procedure takes place across several stages within an IVF cycle:
- Eggs are retrieved from the female partner and fertilised with sperm in the laboratory to create embryos.
- Embryos are grown in culture for approximately 5 to 6 days until they reach the blastocyst stage.
- A trained embryologist gently removes 5 to 6 cells from the outer layer (trophectoderm) of each blastocyst using a fine laser-assisted technique. This outer layer later forms the placenta.
- Immediately after biopsy, each embryo is vitrified (rapidly frozen) to preserve it while testing is underway.
- The biopsied cells are placed in a PGT collection kit and transported on dry ice to the Lupin Diagnostics laboratory for NGS-based chromosomal analysis.
- Results are reported within 18 days. The fertility team then reviews findings and plans the next steps for embryo transfer.
Factors That Can Affect Accuracy
Several technical and biological factors can influence the reliability of results:
- An embryologist's skill and experience during the biopsy procedure
- Quality of the embryo culture environment and equipment
- DNA amplification errors during NGS library preparation
- Poor-quality DNA or insufficient cellular material in the biopsy sample
- Liquid carryover during sample loading into collection tubes
- Laboratory protocols and handling during transit on dry ice
Understanding Your PGT-A (10 Embryos) Test Results
Results are reported for each embryo individually. Your fertility specialist or genetic counsellor will review the findings with you and guide the next steps.
| Result Category | Meaning | Clinical Implication |
|---|---|---|
| Euploid (normal) | Embryo has 46 chromosomes — the correct number | Approximately 60% chance of live birth; recommended for transfer |
| Aneuploid (abnormal) | Extra or missing whole chromosomes or chromosome segments | Live birth rate of 0 to 1%; generally not recommended for transfer |
| Mosaic | Mix of normal and abnormal cells within the same embryo | 30 to 70% of cells abnormal; lower success rate than euploid but healthy live births are possible |
| Inconclusive / No result | Insufficient DNA from the biopsy for analysis | Affects 2 to 7% of samples; re-biopsy may be considered |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
- Maternal age has a direct effect: the rate of chromosomal aneuploidy in embryos is approximately 28% in women under 30, rising to around 67% in older age groups.
- Technical artefacts from DNA amplification errors during NGS can occasionally lead to a false mosaicism finding; your genetic counsellor can help clarify ambiguous results.
- Inconclusive results may occur when the biopsy sample contains poor-quality DNA or an insufficient number of cells for analysis.
How to Maintain Healthy Levels
While no specific "levels" apply to embryo genetic results, these general tips support a positive IVF outcome:
- Maintain a balanced diet and avoid smoking and alcohol during your IVF cycle, as these can affect egg and sperm quality.
- Manage stress through rest, light activity, and support from a counsellor or support group — this is a demanding process.
- A euploid result provides strong reassurance about chromosome count but does not replace first-trimester screening, NIPT, or a mid-pregnancy anomaly scan during the resulting pregnancy.
Lupin Diagnostics PGT-A (10 Embryos) Test Price
The PGT-A test cost for the 10-embryo panel starts at ₹1,00,000 at Lupin Diagnostics. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for embryo biopsy samples.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 100000 |
| CHENNAI | 100000 |
| HYDERABAD | 100000 |
| KOLKATA | 100000 |
| NAVI MUMBAI | 100000 |
| PUNE | 100000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book the PGT-A test:
- Select the PGT-A (10 Embryos) test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample submission; your IVF clinic will coordinate embryo biopsy sample dispatch.
- Receive your report via email or WhatsApp within 18 days.
Frequently Asked Questions
The PGT-A test checks embryos created during IVF for chromosomal abnormalities before transfer. By identifying embryos with the correct number of chromosomes, the test aims to improve the chances of a successful pregnancy and reduce the risk of miscarriage.
At Lupin Diagnostics, results for the PGT-A (10 embryos) test are delivered within 18 days of the laboratory receiving the biopsy sample. Plan your IVF timeline with your fertility specialist accordingly.
You must submit a duly filled Test Request Form, a biopsy worksheet, a signed consent form, and a detailed clinical history, along with past reports. Missing documentation can delay processing, so check with your IVF clinic in advance.
No. The PGT-A test improves the chances of implantation and a healthy pregnancy by selecting chromosomally normal embryos, but it does not guarantee success. A euploid embryo may still not implant due to other factors, such as uterine, immunological, or sperm-related issues.
A mosaic embryo contains a mix of chromosomally normal and abnormal cells. The proportion of abnormal cells influences the outcome. While mosaic embryos have a lower success rate than fully euploid ones, healthy live births following mosaic embryo transfers have been reported. Your genetic counsellor will guide you on the best course of action.
Yes. Trophectoderm biopsy is a well-established procedure. Studies show it does not harm the embryo or the child born from it. The cells removed come from the part of the embryo that forms the placenta, not the inner cell mass that becomes the baby.
Lupin Diagnostics also offers PGT-A panels for 2, 3, and 4 embryos, with pricing adjusted accordingly. If you have fewer embryos to test, speak with the Lupin Diagnostics team to select the panel that matches your cycle outcome.
PGT-A (10 Embryos) Test
