p-ANCA/MPO ELISA Test
About p-ANCA/MPO ELISA Test
| Field | Value |
|---|---|
| Also Known As | MPO-ANCA ELISA, p-ANCA (MPO) ELISA, Myeloperoxidase Antibody Test, Perinuclear ANCA Test, MPO by EIA |
| Sample Type | Serum (venous blood) |
| Fasting Required | No fasting required |
| Report Time | 1 Day |
| Recommended For | Adults of all genders; ordered when vasculitis or autoimmune inflammatory disease is suspected |
| Price | Starting at ₹2,000 |
What is a p-ANCA/MPO ELISA Test?
The p-ANCA/MPO ELISA test is a blood test that detects autoantibodies directed against myeloperoxidase (MPO), an enzyme found inside neutrophils (white blood cells that help fight infection). When the immune system mistakenly produces these antibodies, it can cause inflammation in small blood vessels.
Doctors order this test to investigate suspected autoimmune or inflammatory conditions, particularly those affecting blood vessels, the kidneys, or the lungs. It is also known as the MPO-ANCA ELISA or Myeloperoxidase Antibody Test.
What Does a p-ANCA/MPO ELISA Test Measure?
This test uses the ELISA (enzyme-linked immunosorbent assay) method to identify and measure the level of MPO-targeted antibodies in the blood. The following explains what is being assessed.
| Component | What It Means |
|---|---|
| MPO-ANCA (Myeloperoxidase Antibodies) | Immune proteins that mistakenly target the MPO enzyme inside neutrophils; their presence can indicate autoimmune blood vessel disease |
| p-ANCA Pattern | A perinuclear staining pattern seen when these antibodies bind to MPO; detected and confirmed using ELISA |
Why is a p-ANCA/MPO ELISA Test Done?
Doctors request this test when a patient shows signs that suggest the immune system may be attacking the body's own blood vessels or organs. Below are the main reasons it is ordered.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to request this test:
- Unexplained fever and persistent fatigue
- Blood in urine or reduced urine output
- Unexplained weight loss and muscle aches
- Persistent cough or coughing up blood
- Skin rashes or purplish spots on the skin
Conditions This Test Can Help Detect
This test can help identify a range of conditions. These include:
- Microscopic polyangiitis (MPA), an inflammation of small blood vessels
- Granulomatosis with polyangiitis (GPA), affecting the nose, lungs, and kidneys
- Eosinophilic granulomatosis with polyangiitis (EGPA), linked to asthma and high eosinophil counts
- Interstitial lung disease where underlying vasculitis such as MPA is suspected
- Systemic lupus erythematosus (SLE), where MPO-ANCA antibodies are sometimes found
- Distinguishing ulcerative colitis from Crohn's disease (both types of inflammatory bowel disease)
p-ANCA/MPO ELISA Test for Chronic Disease Monitoring
This test is used to track disease activity and treatment response in patients already diagnosed with MPO-ANCA-associated conditions.
Rising MPO-ANCA levels during follow-up have been linked to clinical worsening in both EGPA and GPA/MPA patient groups.
Conversely, when MPO-ANCA levels drop and remain negative, it is associated with sustained remission.
Doctors typically repeat the test every three to six months during active disease or when treatment is being adjusted.
How to Prepare and What to Expect
No special preparation is needed for the p-ANCA/MPO ELISA test, but there are a few practical steps that will help ensure accurate results.
Do You Need to Fast?
No fasting is required before this test. You can eat and drink normally beforehand. If your doctor has ordered additional tests alongside this one, check whether those require fasting.
Practical Tips Before Your Test
The following steps will help your appointment go smoothly:
- Bring a detailed clinical history including your symptoms, previous test results, and any relevant medical records, as this is required for the test
- Inform your doctor about all medications you are taking, including immunosuppressants, steroids, and supplements such as biotin, as these can affect results
- Specifically mention if you are taking propylthiouracil, hydralazine, or minocycline, as these drugs can cause a false-positive reading
- Drink water normally before the test; no dietary restrictions apply
- Carry your doctor's prescription or referral to the collection centre
Step-by-Step Procedure
Here is what happens during sample collection:
- A phlebotomist (a trained professional who collects blood) ties an elastic band around your upper arm and cleans the skin over a vein inside the elbow.
- A small needle is inserted into the vein. You may feel a brief sting, which passes quickly.
- A small amount of blood (around 3 ml) is drawn into a collection tube.
- The band and needle are removed, and a bandage is placed on the puncture site.
- The serum is separated from the blood sample and stored under refrigeration before dispatch to the laboratory.
- The sample is analysed using the EIA (enzyme immunoassay) method, and the result is reported within one day.
Factors That Can Affect Accuracy
Several factors may influence the reliability of results:
- Certain medications, such as hydralazine, propylthiouracil, and minocycline, can trigger drug-induced MPO-ANCA
- Immunosuppressive drugs may lower antibody levels, affecting the result
- Active infections or other inflammatory conditions can occasionally cause a false-positive reading
- The timing of the test relative to disease activity (active flare versus remission) influences the result
- Differences in laboratory methods and assay kits can affect reported cut-off values
Understanding Your p-ANCA/MPO ELISA Test Results
Results from this test should always be reviewed together with your clinical history and other investigations. The table below shows the general reference ranges used in most laboratories.
| Parameter | Negative (Normal) | Equivocal (Borderline) | Positive |
|---|---|---|---|
| MPO-ANCA (ELISA, common cut-off) | 19 AU/mL or below | 20 to 25 AU/mL | 26 AU/mL or above |
| MPO-ANCA (alternate assay) | Below 5.0 IU/mL | Not applicable | 5.0 IU/mL or above |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain situations can alter how results are interpreted:
- Drugs such as propylthiouracil, hydralazine, minocycline, and sulfasalazine can induce MPO-ANCA in the blood, producing a positive result even without true vasculitis.
- Antinuclear antibodies (ANA), immune proteins involved in other autoimmune conditions, can mimic the p-ANCA pattern on standard immunofluorescence testing; ELISA helps differentiate the two.
- Active infections or generalised inflammation may occasionally produce borderline or weakly positive results that do not reflect a vasculitis diagnosis.
How to Maintain Healthy Levels
These general wellness tips support ongoing immune health:
- Attend regular follow-up appointments if you have a diagnosed autoimmune condition, so any changes in MPO-ANCA levels are detected early
- Report new or worsening symptoms, such as blood in urine, difficulty breathing, or new skin lesions, to your doctor promptly
- Maintain a balanced diet and manage stress levels, as general well being supports immune function
Lupin Diagnostics p-ANCA/MPO ELISA Test Price and Home Collection
The p-ANCA/MPO ELISA test is available at Lupin Diagnostics starting at ₹2,000, with home sample collection offered across cities.
The table below lists approximate prices by city:
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1800 |
| CHENNAI | 1200 |
| HYDERABAD | 1200 |
| KOLKATA | 2000 |
| NAVI MUMBAI | 2000 |
| PUNE | 2000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Booking the p-ANCA/MPO ELISA test is straightforward:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Home Collection
Home blood sample collection for the p-ANCA/MPO ELISA test is available across cities. A certified phlebotomist visits your home, collects the venous blood sample, and transports it safely to the laboratory. All samples are processed in NABL-accredited laboratories, and your digital report is accessible via email or WhatsApp.
Frequently Asked Questions
This test detects autoantibodies that target the myeloperoxidase enzyme in white blood cells. Doctors use it to investigate suspected autoimmune conditions that cause blood vessel inflammation, such as microscopic polyangiitis and granulomatosis with polyangiitis. It is also used to monitor disease activity in patients already diagnosed with these conditions.
No fasting is needed. You can eat and drink normally before the test. If your doctor has ordered other tests at the same time, confirm whether any of those require fasting.
The p-ANCA/MPO ELISA test procedure involves interpreting antibody levels in the context of your symptoms and medical background. A positive result alone is not enough to make a diagnosis; your doctor needs your full clinical picture to arrive at the correct conclusion.
A positive result suggests the presence of MPO-targeted autoantibodies and is consistent with conditions such as microscopic polyangiitis or eosinophilic granulomatosis with polyangiitis, when combined with the right clinical features. However, a positive result on its own is not diagnostic. Your doctor will consider your symptoms, other blood tests, and possibly a tissue biopsy before confirming any diagnosis.
Yes. Propylthiouracil, hydralazine, and minocycline, among other drugs, can trigger the body to produce MPO-ANCA, leading to a positive result that does not reflect true vasculitis. Always give your doctor a full list of medications and supplements before the test.
Yes. In patients diagnosed with MPO-ANCA-associated vasculitis, rising antibody levels during follow-up have been linked to worsening disease. The test may be repeated every three to six months to track how well treatment is working and to detect early signs of a relapse.
Results from Lupin Diagnostics are typically delivered within one day of sample collection. Your report will be sent to you digitally via email or WhatsApp once ready.
p-ANCA/MPO ELISA Test
