Myeloproliferative Neoplasia (MPN) Extended Profile: Booking, Price, and Results
About Myeloproliferative Neoplasia (MPN) Extended Profile: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | MPN Panel, MPN Mutation Panel, Myeloproliferative Neoplasm Profile, JAK2/CALR/MPL Mutation Panel |
| Sample Type | Bone marrow (sodium heparin tube) |
| Fasting Required | No fasting required |
| Report Time | 15 days |
| Recommended For | Adults of any gender with suspected blood cancers or abnormal blood counts |
| Price | Starting at ₹14,000 |
What is a Myeloproliferative Neoplasia (MPN) Extended Profile?
The Myeloproliferative Neoplasia (MPN) Extended Profile is a molecular genetic test that identifies specific mutations in key genes linked to a group of blood cancers. These cancers cause the bone marrow to produce too many blood cells. Doctors order this test when a patient has persistently abnormal blood counts or symptoms that suggest a myeloproliferative neoplasm. The test uses a bone marrow sample and analyses DNA using Sanger sequencing.
What Does a Myeloproliferative Neoplasia (MPN) Extended Profile Measure?
This panel examines mutations in three major driver genes central to the diagnosis of myeloproliferative neoplasms. The following parameters are included:
| Parameter | What It Looks For |
|---|---|
| JAK2 V617F | The most common mutation; found in the majority of polycythemia vera (condition associated with increased red blood cells) cases and in many cases of essential thrombocythaemia (condition associated with platelets) |
| JAK2 Exon 12 | A secondary JAK2 mutation observed in a small proportion of polycythemia vera patients who lack the V617F mutation |
| CALR Exon 9 | Calreticulin gene mutation; found in a significant number of essential thrombocythaemia and primary myelofibrosis patients |
| MPL W515L/K | Thrombopoietin receptor mutation; detected in a smaller subset of essential thrombocythaemia and primary myelofibrosis patients |
| BCR-ABL1 | A fusion gene used to rule out chronic myeloid leukaemia |
Why is a Myeloproliferative Neoplasia (MPN) Extended Profile Done?
This test is ordered when a doctor needs to investigate the cause of abnormal blood cell production or to confirm a suspected blood cancer diagnosis.
Common Symptoms That May Require This Test
Several symptoms may prompt a doctor to request this test. These include:
- Persistent fatigue or tiredness without a clear cause
- Unexplained weight loss
- Abdominal discomfort or a feeling of fullness
- Night sweats or excessive sweating
- Easy bruising or unusual bleeding
- Itching, particularly after a warm bath or shower
- Headache, dizziness, or blurred vision
Conditions This Test Can Help Detect
The MPN test helps identify a range of blood conditions. These include:
- Polycythemia vera (PV), where too many red blood cells are produced
- Essential thrombocythaemia (ET), involving excess platelet production
- Primary myelofibrosis (PMF), where scar tissue forms in the bone marrow
- Chronic myeloid leukaemia (CML), ruled out via the BCR-ABL1 marker
- Chronic neutrophilic leukaemia (CNL)
Myeloproliferative Neoplasia (MPN) Extended Profile for Chronic Disease Monitoring
For patients already diagnosed with an MPN, this panel may be used to track disease progression over time. Changes in mutation levels can indicate whether a treatment is working. Regular monitoring helps the treating doctor make informed decisions about ongoing care.
How to Prepare and What to Expect
This test involves collecting a bone marrow sample, which is performed by a trained medical professional at a diagnostic centre. Here is what you need to know before your appointment.
Do You Need to Fast?
No fasting is required before this test. You may eat and drink normally on the day of your appointment.
Practical Tips Before Your Test
Being prepared helps the collection go smoothly. Keep the following in mind:
- Bring a detailed clinical history, including your symptoms, previous test results, and any relevant medical records, as this is required for the test
- Carry your doctor's prescription and any prior blood count reports
- Inform the laboratory staff about all current medications, especially blood thinners or chemotherapy drugs
- Stay well-hydrated before your appointment
- Wear comfortable, loose-fitting clothing that allows easy access to the collection area
Step-by-Step Procedure
- You check in at the Lupin Diagnostics centre and submit your clinical history and prescription.
- A trained medical professional explains the bone marrow collection process and answers any questions you have.
- The collection site, usually the hip bone (posterior iliac crest), is cleaned with an antiseptic solution.
- A local anaesthetic is applied to numb the area and minimise discomfort.
- A small sample of bone marrow is drawn using a specialised needle and collected into a sodium heparin tube.
- The sample is labelled, stored at 2 to 8 degrees Celsius, and dispatched to the molecular diagnostics laboratory for Sanger sequencing analysis.
Factors That Can Affect Accuracy
Certain factors may influence the reliability of your results. These include:
- Low number of mutated cells in the sample, which can sometimes go undetected
- Recent blood transfusions, which may dilute the mutation signal
- Current treatment with JAK inhibitors or chemotherapy, which may reduce detectable mutation levels
- Improper sample storage or handling during transport
- Inadequate sample volume collected
Understanding Your Myeloproliferative Neoplasia (MPN) Extended Profile Results
Your results will show whether each tested mutation was detected. A doctor, ideally a haematologist, should always review these results alongside your full blood count and clinical history.
| Parameter | Normal Result | Positive Result (Detected) |
|---|---|---|
| BCR-ABL1 | Not detected | Supports diagnosis of CML |
| JAK2 V617F | Not detected | Strongly supports PV; also seen in ET and PMF |
| JAK2 Exon 12 | Not detected | Supports PV in JAK2 V617F-negative patients |
| CALR Exon 9 | Not detected | Associated with ET or PMF; linked to a more favourable outcome in PMF |
| MPL Exon 10 | Not detected | Supports diagnosis of ET or PMF |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
A few situations can affect how results should be read. If only a small proportion of cells carry the mutation, the test may return a negative result even when a mutation is present. Patients who have recently received a blood transfusion may also have results that do not fully reflect their underlying condition. Additionally, CALR, JAK2, or MPL mutations are mutually exclusive, meaning detection of one typically indicates the others are absent. Your doctor will take these factors into account.
How to Maintain General Wellness
- Attend all scheduled follow-up appointments so your doctor can track any changes in your condition
- Stay well-hydrated each day to support healthy blood circulation
- Report any new or worsening symptoms, such as persistent fatigue, unexplained bruising, or night sweats, to your doctor without delay
Lupin Diagnostics Myeloproliferative Neoplasia (MPN) Extended Profile Price and Home Collection
The MPN test cost at Lupin Diagnostics starts at ₹14,000. This test is conducted at the centre/hospital, with no home sample option.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 14000 |
| CHENNAI | 14000 |
| HYDERABAD | 14000 |
| KOLKATA | 14000 |
| NAVI MUMBAI | 14000 |
| PUNE | 14000 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
- Select the Myeloproliferative Neoplasia (MPN) Extended Profile on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The Myeloproliferative Neoplasia (MPN) Extended Profile detects specific gene mutations (JAK2, CALR, MPL, and BCR-ABL1) that help doctors diagnose myeloproliferative neoplasms, a group of blood cancers where the bone marrow overproduces blood cells. It is ordered when blood counts are persistently abnormal or when clinical symptoms point to this group of conditions.
This test is typically recommended by doctors for adults with significantly elevated red blood cell counts, haemoglobin, haematocrit, or platelet levels who are suspected of having a myeloproliferative neoplasm. It is also used for patients already diagnosed with an MPN who need ongoing monitoring.
No fasting is required. You can eat and drink as normal before your appointment. However, do bring your complete clinical history and doctor's prescription, as these are required for the test to be processed.
The report is delivered within 15 days of sample collection. This time is needed for DNA extraction, Sanger sequencing, data analysis, and expert interpretation of the results.
A detected mutation is considered a positive result and is strongly associated with a myeloproliferative neoplasm. However, a positive finding must be reviewed alongside your blood counts, clinical symptoms, and possibly bone marrow examination before a definitive diagnosis is made. Your haematologist will guide you on the next steps.
Not necessarily. A small proportion of MPN patients do not carry detectable JAK2, CALR, or MPL mutations (referred to as triple-negative cases). If your result is negative but symptoms persist, your doctor may recommend further tests, including a bone marrow biopsy, to complete the evaluation.
Yes, the price may differ slightly depending on the city and the specific centre. The starting price at Lupin Diagnostics is ₹14,000. It is advisable to confirm the exact price at the time of online booking for the MPN test.
Myeloproliferative Neoplasia (MPN) Extended Profile: Booking, Price, and Results
