MTB Susceptibility – Amikacin Test: Booking, Price, and Results
About MTB Susceptibility – Amikacin Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | AFB-MTB Antibiogram Amikacin, TB Drug Susceptibility Test – Amikacin, MTB-Amikacin Sensitivity Test, AFB Susceptibility – Amikacin |
| Sample Type | Aspirate, Bronchoalveolar Lavage (BAL), Bile, Bone Marrow, Bronchial Fluid, Catheter Tip, Corneal Scraping, Cerebrospinal Fluid (CSF), Endotracheal Secretion, Gastric Fluid, Hair Clipping, Liver Abscess Fluid, Nail, Pericardial Fluid, Peritoneal Fluid, Pleural Fluid |
| Fasting Required | No fasting required |
| Report Time | 29 days |
| Recommended For | All genders and age groups with confirmed or suspected drug-resistant tuberculosis |
| Price | Starting at ₹2,200 |
What Is an MTB Susceptibility – Amikacin Test?
The MTB Susceptibility – Amikacin test checks whether the bacterium causing tuberculosis (TB), Mycobacterium tuberculosis, responds to the antibiotic Amikacin. It is an antibiogram test, meaning it maps bacterial sensitivity or resistance to a specific drug. Amikacin is a second-line injectable drug used when standard TB medicines stop working. The test is also called the AFB-MTB Antibiogram Amikacin or the TB Drug Susceptibility Test – Amikacin. It is prescribed for patients who are suspected of having drug-resistant TB and uses a pure culture grown from a clinical sample such as sputum or body fluid. Doctors order this test for patients diagnosed with TB, particularly when drug-resistant TB is suspected.
What Does an MTB Susceptibility – Amikacin Test Measure?
This test analyses how the Mycobacterium tuberculosis (MTB) strain in a sample reacts when exposed to Amikacin. The result tells doctors whether the drug will be effective or whether the bacteria have developed resistance.
| Component | What It Tells You |
|---|---|
| Amikacin sensitivity or resistance | Whether the TB strain will respond to Amikacin treatment |
| Minimum inhibitory concentration (MIC) | The lowest Amikacin concentration that stops visible bacterial growth |
| Second-line drug effectiveness | Whether Amikacin can be safely included in the treatment plan |
The test uses a critical concentration of 1.0 mg/L to decide whether a strain is sensitive or resistant. The median minimum inhibitory concentration (MIC, meaning the lowest drug concentration that stops bacterial growth) for Amikacin is 2 mg per litre.
Why Is an MTB Susceptibility – Amikacin Test Done?
This test is ordered when a patient has TB and the treating doctor needs to confirm which drugs can effectively fight the infection. The MTB Susceptibility – Amikacin test procedure is particularly important for patients who have not responded to standard first-line TB treatment.
Common Symptoms That May Require This Test
A doctor may order this test when a TB patient shows any of the following symptoms:
- Cough lasting more than three weeks
- Chest pain
- Persistent fever and chills
- Night sweats
- Unexplained weight loss
- Loss of appetite
- Unusual fatigue or weakness
- Failure to improve on standard TB medications
Conditions This Test Can Help Detect
The test helps identify and manage several serious conditions. These include:
- Multidrug-resistant TB (MDR-TB): TB that is resistant to at least isoniazid and rifampicin, requiring second-line treatment with drugs like Amikacin.
- Extensively drug-resistant TB (XDR-TB): TB that is resistant to isoniazid and rifampicin, at least one fluoroquinolone, and one or more injectable second-line drugs, including Amikacin.
- Resistance to second-line injectable drugs, guiding selection of an effective treatment regimen
- Acquired drug resistance arising from incomplete or prior TB treatment
MTB Susceptibility – Amikacin Test for Chronic Disease Monitoring
Patients on MDR-TB treatment regimens undergo regular susceptibility testing to track how well the treatment is working. Drug susceptibility testing should be repeated if a patient shows a positive culture after three months of treatment or does not appear to be improving. This helps detect any newly emerging resistance and allows the doctor to adjust the treatment plan accordingly.
How to Prepare and What to Expect
No special preparation is needed for the MTB Susceptibility – Amikacin test. The collection process will depend on the type of sample your doctor has ordered.
Do You Need to Fast?
No, fasting is not required for this test. You can eat and drink normally before sample collection.
Practical Tips Before Your Test
The following steps will help ensure accurate results:
- Bring a detailed clinical history, including your symptoms, previous test results and family history, as this is required for the test
- If providing a sputum sample, rinse your mouth thoroughly with water beforehand
- Inform your doctor about all medications and supplements you are currently taking
- For a sputum sample, aim for a deep cough to produce secretions from the lower respiratory tract rather than saliva; try to collect it early in the morning when bacterial load in the airways is typically higher
- Wear comfortable clothing to your appointment
- Follow any specific instructions given by your doctor or the collection team
- Mention your complete address and mobile number so that the report can be shared as soon as it is ready.
Step-by-Step Procedure
The MTB Susceptibility – Amikacin test procedure involves growing the TB bacteria in a controlled setting before testing their response to Amikacin. Here is what the process involves:
- Your doctor identifies the most appropriate sample type based on your clinical condition. This may be sputum, bronchial lavage (BAL) fluid, urine, pus or tissue.
- The sample is collected using the appropriate method, such as deep coughing for sputum or a clinical procedure for other body fluids or tissues.
- The collected sample is placed in a sterile container and stored at 2 to 8 degrees Celsius for safe transport to the laboratory.
- In the laboratory, Mycobacterium tuberculosis is grown from the sample in either solid or liquid culture media to produce a pure culture.
- The pure culture is exposed to Amikacin at its critical concentration using an automated system. If 1% or more of the bacterial population continues to grow, the strain is classified as resistant.
- Results are reported as "Sensitive" or "Resistant" and dispatched within the stated turnaround time.
Factors That Can Affect Accuracy
Several factors may influence the reliability of the test result:
- Quality and quantity of the specimen collected
- Proper storage and transport of the sample at the required temperature
- Prior use of anti-TB medications before sample collection
- Mixed bacterial contamination in the specimen
- Laboratory technique and equipment calibration
Understanding Your MTB Susceptibility – Amikacin Test Results
Results for this test are straightforward and reported as either 'sensitive' or 'resistant'. Always review your results with your treating doctor, who will interpret them alongside your full clinical picture.
| Parameter | Result | Interpretation |
|---|---|---|
| Amikacin susceptibility | Sensitive (S) | The TB bacteria will respond to Amikacin; it can be included in the treatment regimen |
| Amikacin susceptibility | Resistant (R) | The TB bacteria will not respond to Amikacin; an alternative drug is needed |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain situations may influence how results are interpreted:
- Patients with a history of incomplete TB treatment may show acquired resistance to Amikacin due to prior antibiotic exposure.
- A resistant result may also indicate cross-resistance to other second-line injectable drugs such as Kanamycin and Capreomycin, which your doctor will consider when planning treatment.
- There can be variation in susceptibility between individual cultures, making it important to consider results alongside clinical findings.
How to Maintain Healthy Levels
While this test is used in the context of active disease, these general wellness tips support TB treatment outcomes:
- Complete the full prescribed course of TB treatment without stopping early, even if you feel better.
- Follow up with your doctor regularly so that treatment response can be monitored and adjustments made if needed.
- Maintain good nutrition and rest to support your body's immune response during treatment.
- Ensure good ventilation in living and working spaces, as TB spreads through the air via respiratory droplets.
Lupin Diagnostics MTB Susceptibility – Amikacin Test Price
The MTB Susceptibility – Amikacin test cost at Lupin Diagnostics starts at ₹2,200. This test requires a visit to a Lupin Diagnostics centre, as home collection is not available for specialised culture-based tests of this type.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 2200 |
| CHENNAI | 2200 |
| HYDERABAD | 2200 |
| KOLKATA | 2200 |
| NAVI MUMBAI | 2200 |
| PUNE | 2200 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Use these steps to book your MTB Susceptibility – Amikacin test online booking:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The MTB Susceptibility – Amikacin test is an antibiotic susceptibility test that checks whether the tuberculosis-causing bacterium responds to the antibiotic Amikacin. It is used in patients with confirmed TB, particularly when drug-resistant TB is suspected, to help doctors choose the right treatment drugs.
Drug resistance can only be confirmed through laboratory susceptibility testing. The result of the AFB-MTB Antibiogram Amikacin test tells doctors whether Amikacin is a viable option for treating a patient's TB, especially in cases of multidrug-resistant or extensively drug-resistant TB.
A pure culture of Mycobacterium tuberculosis grown from various clinical specimens is used. Acceptable sample types include sputum, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), pus, tissue biopsy, lymph node material and urine. Your doctor will advise which sample type is appropriate for your situation.
The standard report time at Lupin Diagnostics is 29 days. The process takes longer than most routine tests because the TB bacteria need time to grow in the laboratory before susceptibility can be assessed. Your doctor will advise on the expected timeline.
A resistant result means the TB bacteria will not respond to Amikacin. Your doctor will review other susceptibility results to select alternative antibiotics that can effectively treat your infection. This does not mean TB cannot be treated; it means a different drug combination is needed.
No fasting or special preparation is required. However, you must bring a detailed clinical history to your appointment, as this is required for the test. If a sputum sample is being collected, rinse your mouth with plain water beforehand and try to produce a deep cough.
Amikacin is a second-line injectable antibiotic used in the management of drug-resistant TB. It works against Mycobacterium tuberculosis in a concentration-dependent manner and is commonly used when first-line drugs such as isoniazid and rifampicin are no longer effective.
MTB Susceptibility – Amikacin Test: Booking, Price, and Results
