IHC Marker-HCG Test: Booking, Price, and Results

The IHC marker-HCG test is a specialised laboratory test that detects the presence of human chorionic gonadotropin (HCG) protein directly within tissue samples. It uses a technique called immunohistochemistry (IHC), where antibodies bind to HCG protein in the tissue and produce a visible colour change under a microscope. Doctors order this test when a biopsy or surgical specimen needs to be examined for trophoblastic disease, germ cell tumours, or certain other cancers. It is also referred to as the Beta-HCG Immunostain or HCG Immunostain.

Doctors request the IHC marker-HCG test when tissue analysis is needed to confirm or rule out specific tumour types. The test provides information that cannot be obtained from blood tests or imaging alone.

Common Symptoms That May Require This Test

• An abnormal tissue mass or lump identified during a physical examination or imaging
• Unexplained or abnormal bleeding, particularly from the uterus
• A suspected tumour on imaging that requires further characterisation
• Swelling in the testicular region
• Previously diagnosed cancer that requires more precise subtyping
• Abnormal uterine bleeding without a clear cause

Conditions This Test Can Help Detect

• Gestational trophoblastic disease, including complete and partial hydatidiform moles
• Gestational and testicular choriocarcinoma
• Placental site trophoblastic disease
• Germ cell tumours of the testis or ovary with a choriocarcinoma component
• Ectopic HCG production in non-trophoblastic cancers such as those of the colon, prostate, bladder, breast, and lung

IHC Marker-HCG Test for Chronic Disease Monitoring

In conditions such as gestational trophoblastic disease and germ cell tumours, HCG is produced in excess by abnormal cells. This makes it useful not only for initial diagnosis but also for tracking how well treatment is working. In some cases, IHC may be repeated on new tissue samples to assess whether the tumour has responded to therapy or whether the disease has recurred.

1. The tissue sample is collected by a qualified doctor through a biopsy or surgical excision at a clinical facility.
2. The sample is preserved in formalin and embedded in paraffin wax to create a stable tissue block (FFPE block). This prevents degradation and allows thin slices to be cut.
3. The laboratory cuts very thin sections from the block and places them on glass slides.
4. Antibodies specific to HCG protein are applied to the slides. Where HCG is present, the antibodies bind to it.
5. A coloured dye is used to make the antibody-bound areas visible under a microscope, producing a brownish colouration in positive areas.
6. A qualified pathologist examines the stained slides, scores the staining intensity, and prepares the final report.

Results from the IHC marker-HCG test must always be reviewed by a qualified pathologist alongside your full clinical picture. The table below outlines how findings are generally interpreted.

These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.

Results During Special Conditions

Certain situations can make results harder to interpret. Current HCG antibodies may produce variable or intense background staining in trophoblastic elements and surrounding tissues, which can complicate the assessment of true-positive areas. Additionally, some tumours can produce elevated serum HCG levels even when tissue staining is negative, so IHC results should always be considered alongside serum HCG measurements and other markers.

The IHC marker-HCG test cost at Lupin Diagnostics starts at ₹1,920. This test requires a visit to a Lupin Diagnostics centre or submission of a properly prepared tissue block through your treating doctor. Home collection is not available for this test.

Prices are indicative and may vary by location. Please confirm the current price at the time of booking.