IHC Marker-EGFR Test: Booking, Price, and Results
About IHC Marker-EGFR Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | EGFR IHC, Epidermal Growth Factor Receptor Immunohistochemistry, EGFR Expression Test, EGFR Protein Expression Test |
| Sample Type | Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Fasting Required | No — this test uses preserved tissue samples, not blood or urine |
| Report Time | 7 days |
| Recommended For | Adults of all genders diagnosed with or suspected of having solid tumours |
| Price | Starting at ₹3,500 |
What is an IHC Marker-EGFR Test?
The IHC Marker-EGFR Test is a specialised laboratory test that detects the presence and level of Epidermal Growth Factor Receptor (EGFR) protein in tumour tissue. EGFR is a protein found on the surface of cells that controls cell growth and division. The test uses an immunohistochemistry (IHC) technique, where specific antibodies are applied to thin slices of tumour tissue to reveal EGFR protein levels.
Doctors order this test when a cancer diagnosis has already been made or is suspected, to guide treatment decisions. It is also known as EGFR IHC or the EGFR Expression Test.
What Does an IHC Marker-EGFR Test Measure?
The IHC Marker-EGFR Test procedure examines two key aspects of the EGFR protein in tumour tissue.
| Parameter | What It Assesses |
|---|---|
| EGFR protein expression | Whether the EGFR protein is present on tumour cell surfaces |
| Staining intensity | Scored as 0 (none), 1 (weak), 2 (moderate), or 3 (strong) |
| Percentage of stained cells | Proportion of tumour cells showing EGFR staining at each intensity level |
| H-score | A combined score from 0 to 300 is calculated from intensity and percentage data |
Together, these measurements tell the pathologist how much EGFR the tumour is producing and whether this is likely to influence treatment choices.
Why is an IHC Marker-EGFR Test Done?
This test is ordered when a doctor needs to understand a tumour's biological profile to plan the most appropriate care.
Common Symptoms That May Require This Test
A doctor may order an EGFR IHC test after a cancer diagnosis is confirmed. The following symptoms often prompt initial investigation and biopsy:
- Unexplained or rapid weight loss
- Persistent cough that does not resolve
- Shortness of breath or difficulty breathing
- Blood in sputum
- Difficulty swallowing
- Persistent abdominal pain or a change in bowel habits
- A palpable lump or mass anywhere in the body
Conditions This Test Can Help Detect
The IHC Marker-EGFR Test is relevant across a range of solid tumour types. These include:
- Non-small cell lung cancer (NSCLC)
- Colorectal cancer
- Head and neck squamous cell carcinoma
- Triple-negative breast cancer
- Ovarian, cervical, bladder, and prostate cancers
- Glioblastoma multiforme (brain tumour)
EGFR overexpression in these cancers is associated with a more aggressive disease course and can indicate eligibility for anti-EGFR targeted therapies.
How to Prepare and What to Expect
Since this test is performed on tissue that has already been collected during a biopsy or surgery, the patient does not need to undergo any new sample collection for the test itself.
Do You Need to Fast?
No fasting is required. The test is performed on a preserved tissue block, so your eating and drinking habits do not affect results.
Practical Tips Before Your Test
The following steps will help ensure the test runs smoothly:
- Bring the formalin-fixed paraffin-embedded (FFPE) tissue block from your biopsy or surgical procedure
- Attach the original histopathology report from your initial tissue examination
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Provide all relevant radiological reports and treatment records
- Inform the lab staff about all medications you are currently taking
Step-by-Step Procedure
The EGFR IHC test procedure involves several laboratory steps performed on the tissue sample:
- The tissue sample is obtained through a prior biopsy or surgical resection by your treating doctor.
- The collected tissue is placed in a formalin solution to preserve it, then processed and embedded in paraffin wax to form a solid block.
- The laboratory cuts extremely thin slices from the FFPE block and places them on microscope slides.
- A specific antibody that binds to the EGFR protein is applied to the slides, along with a coloured dye, so the binding can be seen under a microscope.
- A trained pathologist examines the stained slides, scoring the intensity and proportion of EGFR-positive tumour cells.
- The results are compiled into a report and dispatched within the turnaround time.
Factors That Can Affect Accuracy
Several factors can influence the reliability of results. These include:
- Inadequate formalin fixation time before the tissue block was prepared
- Low tumour cell content in the sample
- Variability in antibody selection or staining methods
- Absence of standardised scoring criteria across laboratories
- Tissue heterogeneity within the tumour
- Experience level of the interpreting pathologist
Understanding Your IHC Marker-EGFR Test Results
Results of the EGFR Protein Expression Test are interpreted by a pathologist in the context of the patient's full clinical picture. The two main scoring methods used are shown below.
| Parameter | Result | Interpretation |
|---|---|---|
| H-score | Less than 200 | Low or negative EGFR expression |
| H-score | 200 or above | High or positive EGFR expression |
| Membrane staining | Less than 1% of tumour cells stained | Negative |
| Membrane staining | 1% or more of tumour cells stained | Positive |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain circumstances can affect the accuracy of IHC results. Tissue samples that were not fixed in formalin promptly after collection may show unreliable staining, as underfixation affects antigen preservation more than overfixation. Variation in laboratory techniques and scoring approaches can also introduce differences between results from different facilities.
How to Maintain Healthy Levels
While EGFR expression is not directly modifiable like blood glucose or cholesterol, these general wellness habits support overall health:
- Follow a balanced diet rich in vegetables, fruits, and whole grains, and maintain a healthy body weight
- Avoid smoking, as tobacco use is a known risk factor for several cancers, where EGFR overexpression is common
- Attend all follow-up appointments scheduled by your oncologist and report any new symptoms promptly
Lupin Diagnostics IHC Marker-EGFR Test Price
The IHC Marker-EGFR Test cost at Lupin Diagnostics starts at ₹3,500. This test requires a visit to a Lupin Diagnostics centre; home collection is not available, as it involves submission of a tissue block prepared during a clinical procedure.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 3500 |
| CHENNAI | 3500 |
| HYDERABAD | 3500 |
| KOLKATA | 3500 |
| NAVI MUMBAI | 3500 |
| PUNE | 3500 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The steps below explain how to book your IHC Marker-EGFR Test online with Lupin Diagnostics:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time to submit your FFPE tissue block and accompanying clinical documents.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The IHC Marker-EGFR Test is a laboratory test that measures the level of EGFR protein in tumour tissue using an immunohistochemistry technique. It helps doctors understand a tumour's biological characteristics and decide whether certain targeted treatments are appropriate.
EGFR controls how cells grow and divide. When a tumour overexpresses this protein, it may respond to specific anti-EGFR therapies. Knowing the EGFR status helps oncologists choose treatments that are more likely to be effective for that particular tumour type.
This EGFR IHC test requires a formalin-fixed paraffin-embedded (FFPE) tissue block, obtained through a prior biopsy or surgical procedure. A blood sample cannot substitute for a tissue block in this test.
No. Because the test uses tissue blocks prepared during a biopsy or surgery, the sample must be submitted at a Lupin Diagnostics centre. Home collection is not available for this test.
The report is typically ready within 7 days of the sample reaching the laboratory. Complex cases may occasionally take a little longer.
No, these are different tests. The IHC Marker-EGFR Test measures how much EGFR protein the tumour is producing, while EGFR mutation testing uses genetic techniques to look for specific changes in the EGFR gene. Both tests provide different but complementary information for treatment planning.
Bring the original FFPE tissue block, the histopathology report from the initial biopsy, all relevant clinical notes and radiology reports, and a detailed clinical history. Clinical history is required for this test, so complete documentation helps ensure accurate reporting.
IHC Marker-EGFR Test: Booking, Price, and Results
