IHC Marker - CD45RB Test: Booking, Price, and Results
About IHC Marker - CD45RB Test
| Field | Value |
|---|---|
| Also Known As | CD45RB Immunohistochemistry, CD45RB IHC, LCA (Leukocyte Common Antigen) Marker, CD45 B-Cell Marker |
| Sample Type | Formalin-fixed, paraffin-embedded (FFPE) tissue block |
| Fasting Required | No fasting required |
| Report Time | 3 days |
| Recommended For | All genders, all ages (when clinically indicated for suspected haematological malignancy) |
| Price | Starting at ₹1,920 |
What Is an IHC Marker - CD45RB Test?
The CD45RB test is a specialised laboratory procedure that uses antibodies to detect the CD45RB protein in tissue samples. CD45RB is a specific form of the leukocyte common antigen (LCA), a protein found on most blood and immune cells. Pathologists use this CD45RB IHC test to help identify and classify tumours, particularly lymphomas and leukaemias. The test is performed on a formalin-fixed, paraffin-embedded (FFPE) tissue block obtained through a biopsy.
What Does an IHC Marker - CD45RB Test Measure?
This test examines tissue under a microscope after applying a special antibody stain. The following markers are assessed:
| Component | What It Detects |
|---|---|
| CD45RB protein expression | Identifies whether cells carry the CD45RB variant of the leukocyte common antigen |
| B-lymphocyte marker expression | Detects mature B-lymphocytes (a type of white blood cell) and most B-cell lymphomas and leukaemias |
| T-cell receptor signalling pattern | Helps assess stages of T-cell development, as CD45RB levels fall as T cells mature |
Why Is an IHC Marker - CD45RB Test Done?
Doctors order this test when they need to determine whether abnormal cells in a tissue sample are of blood or immune cell origin.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to request a biopsy and this IHC marker:
- Enlarged lymph nodes, felt as lumps in the neck, underarm, or groin
- Unexplained weight loss
- Persistent fatigue (feeling very tired) without a clear reason
- Fever and chills with no obvious cause
- Swollen abdomen or feeling full after eating very little
- Chest pain or pressure
- Night sweats
Conditions This Test Can Help Detect
This test helps doctors investigate a range of blood cell-related conditions, including:
- Malignant lymphomas (cancers arising from lymphocytes)
- Leukaemias (cancers of blood-forming cells)
- Hodgkin lymphoma subtypes, including classic Hodgkin lymphoma and nodular lymphocyte predominant Hodgkin lymphoma
- Mast cell neoplasms and histiocytic or dendritic cell tumours
- Differentiation of haematolymphoid tumours from epithelial cancers, melanoma, germ cell tumours, and sarcoma
How to Prepare and What to Expect
No specific preparation is needed from the patient before the biopsy. However, there are several important things to arrange beforehand.
Do You Need to Fast?
No, fasting is not required for this test. The test is performed on a tissue sample, not on blood or urine, so there are no dietary restrictions.
Practical Tips Before Your Test
Keep the following in mind before your appointment:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test.
- Bring your original histopathology report and all relevant clinical and radiological records.
- Inform your doctor about any medications you are currently taking.
- Let your doctor know if you have received chemotherapy or radiation therapy, as these may affect test results.
- The biopsy procedure is carried out in a medical setting by a trained healthcare professional.
Step-by-Step Procedure
The CD45RB procedure involves two main stages: tissue collection (biopsy) and laboratory processing:
- A doctor cleans the area and applies a local anaesthetic (a numbing agent) to minimise discomfort before the biopsy.
- A small piece of tissue is removed using a needle, incision, or surgical method, depending on the location of the suspected abnormality.
- The tissue is placed in formalin to preserve it, then embedded in a paraffin block to create a stable FFPE sample.
- In the laboratory, thin sections of the tissue block are placed on microscope slides and treated with a CD45RB-specific antibody.
- A coloured dye (chromogen) marks any cells that carry the CD45RB protein, making them visible under a microscope.
- A pathologist examines the staining pattern and correlates the findings with your clinical history and other test results before issuing a report.
Factors That Can Affect Accuracy
- Poor tissue fixation (if the biopsy sample was not preserved quickly or correctly)
- Delays between tissue removal and processing
- Prior chemotherapy or radiation, which can alter protein expression in cells
- The age and type of the tissue sample
- Laboratory technical factors, including antibody quality and equipment calibration
Understanding Your IHC Marker - CD45RB Test Results
Results are reported as positive or negative staining and must always be read alongside your full clinical picture. The table below provides a general guide to interpreting staining patterns:
| Staining Result | What It May Indicate |
|---|---|
| Positive (membranous or cytoplasmic staining) | Cells are of haematolymphoid (blood or immune cell) origin; consistent with lymphoma, leukaemia, or related conditions |
| Negative | Cells may be of non-haematolymphoid origin, or the tumour may be a subtype known to lose CD45 expression (e.g., classical Hodgkin lymphoma, multiple myeloma) |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain conditions can produce results that differ from the typical pattern.
- Classical Hodgkin lymphoma is characterised by an absence of CD45 staining on Reed-Sternberg cells, even though the tumour is of lymphoid origin. In contrast, the L&H cells in the lymphocyte-predominant type of Hodgkin lymphoma are consistently CD45 positive.
- Aberrant CD45 expression in non-haematolymphoid tumours (such as carcinomas) is possible but very uncommon. A pathologist will correlate the staining result with other IHC markers and clinical findings before drawing conclusions.
How to Maintain Healthy Levels
Because this is a diagnostic marker rather than a routine health measurement, "maintaining levels" is not applicable in the usual sense. These general tips support overall health:
- Eat a balanced diet with plenty of fruits, vegetables, and whole grains to support your immune system.
- Report persistent symptoms such as unexplained swollen lymph nodes, fatigue, or night sweats to your doctor promptly.
- Attend regular health check-ups as recommended by your healthcare provider.
Lupin Diagnostics IHC Marker - CD45RB Test Price
The CD45RB test cost at Lupin Diagnostics starts at ₹1,920. As this is a specialised test requiring laboratory processing of a tissue block, it must be processed at a Lupin Diagnostics centre. Home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1920 |
| CHENNAI | 1920 |
| HYDERABAD | 1920 |
| KOLKATA | 1920 |
| NAVI MUMBAI | 1920 |
| PUNE | 1920 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your CD45RB online booking with Lupin Diagnostics:
- Select the IHC Marker - CD45RB Test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample submission.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
This test detects the CD45RB protein in tissue samples using a specialised antibody. It helps pathologists determine whether abnormal cells come from blood or immune cells, which is critical for identifying and classifying lymphomas and leukaemias. Results are always used alongside other clinical and laboratory findings.
The sample comes from a biopsy, in which a doctor removes a small piece of tissue from the affected area. The method used (needle, incision, or surgical) depends on the location of the tissue being examined. The tissue is then processed into a paraffin block before testing.
A positive result confirms that the cells in the sample are of blood or immune cell origin. It does not diagnose cancer on its own. Your doctor will review the CD45RB IHC result alongside other markers, imaging findings, and your clinical history before reaching a diagnosis.
Although CD45 is present in most haematolymphoid tumours, classical Hodgkin lymphoma is a well-known exception. The Reed-Sternberg cells characteristic of this condition do not express CD45. Precursor B-lymphoblastic leukaemias and most cases of multiple myeloma also tend to be CD45 negative.
The CD45RB test can confirm that a tumour is of lymphoid origin, but it cannot distinguish between B-cell and T-cell subtypes on its own. Additional IHC markers are needed to identify the specific type of lymphoma.
No fasting or medication restrictions are needed before the biopsy. However, you should carry your complete clinical history, previous test reports, and radiological records, as these are required for accurate interpretation of the CD45RB test results.
IHC Marker - CD45RB Test: Booking, Price, and Results
