IHC Marker - CD45 Test: Booking, Price, and Results
About IHC Marker - CD45 Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD45 LCA, Leukocyte Common Antigen (LCA) IHC, CD45 Immunohistochemistry, CD45 Immunostain, PTPRC |
| Sample Type | Tissue biopsy (formalin-fixed, paraffin-embedded tissue block) |
| Fasting Required | No |
| Report Time | 7 days |
| Recommended For | All ages and genders when a haematological malignancy or tumour of unknown origin is suspected |
| Price | Starting at ₹1,920 |
What is an IHC Marker - CD45 Test?
The IHC marker - CD45 test is a specialised pathology test that uses targeted antibodies to detect the CD45 protein in a tissue sample. CD45, also known as Leukocyte Common Antigen (LCA), is a protein found exclusively on blood-forming cells. Doctors order this test to help identify and classify blood-related cancers such as lymphomas and leukaemias. The sample used is a formalin-fixed, paraffin-embedded (FFPE) tissue block obtained through a biopsy.
What Does an IHC Marker - CD45 Test Measure?
The IHC marker - CD45 test procedure involves staining tissue with CD45-specific antibodies and examining the result under a microscope. The test produces a qualitative result — the CD45 protein is either present or absent in the tissue.
| Parameter | Result Type | What it Indicates |
|---|---|---|
| CD45 Expression | Positive or Negative | Whether the tissue contains cells of haematopoietic (blood-forming) origin |
A positive result indicates the cells are likely of blood-cell origin, which is critical for diagnosing conditions such as lymphoma or leukaemia. A negative result suggests the abnormal cells are not blood-derived, helping to rule out these conditions or point towards other tumour types.
Why is an IHC Marker - CD45 Test Done?
A pathologist or treating doctor may request this test when there are signs that a tumour or abnormal tissue could be of haematolymphoid origin. The test helps distinguish blood-related cancers from other types.
Common Symptoms That May Require This Test
The following symptoms may lead a doctor to investigate for a haematological malignancy:
- Swollen lymph nodes in the neck, armpits, or groin
- Extreme or persistent fatigue
- Unexplained weight loss
- Night sweats
- Pallor (pale skin) or anaemia
- Persistent fever without a clear cause
- Frequent or unusual infections
Conditions This Test Can Help Detect
The CD45 IHC test can help identify or rule out the following conditions:
- Non-Hodgkin lymphoma and its various subtypes
- Leukaemias and other blood-related cancers
- Tumours of haematopoietic origin
- Inflammatory cell infiltration in tissue
- Poorly differentiated tumours where the cell origin is unclear
How to Prepare and What to Expect
Because the IHC marker - CD45 test is performed on a tissue sample rather than a blood or urine sample, there is very little you need to do beforehand. However, the information you provide your doctor is just as important as the sample itself.
Do You Need to Fast?
No fasting is required for this test. The test is performed on a tissue biopsy, and no dietary restrictions apply.
Practical Tips Before Your Test
Here are a few things to keep in mind before the test:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Inform your doctor of all medications you are currently taking
- Share any previous biopsy reports or existing tissue blocks from prior procedures
- Ask your doctor to explain the biopsy procedure, so you know what to expect
Step-by-Step Procedure
The following steps describe how the sample is collected and processed for the IHC marker - CD45 test:
- Your doctor identifies the area of tissue to be biopsied based on your symptoms and imaging results.
- The skin or area around the biopsy site is cleaned, and a local anaesthetic is applied to numb the area.
- A small piece of tissue is removed using a needle, surgical incision, or a similar tool, depending on the location.
- The tissue is placed in formalin to preserve it and then embedded in paraffin wax to create a tissue block (FFPE block).
- Thin sections are cut from the block, placed on slides, and stained with CD45-specific antibodies in the laboratory.
- A qualified pathologist examines the stained slides under a microscope and documents whether CD45 expression is present or absent.
Factors That Can Affect Accuracy
The following factors may affect the reliability of the test result:
- Improper tissue fixation or processing after the biopsy
- Insufficient tissue in the sample
- Poor quality of the antibody reagent used
- Incorrect labelling or handling of the tissue block
- Variation in pathologist interpretation, particularly in borderline or poorly defined tumours
Understanding Your IHC Marker - CD45 Test Results
Results from the IHC marker - CD45 test are qualitative and must be read alongside your full clinical picture. A pathologist will prepare a report, but your treating doctor will explain what the result means for you specifically.
| Parameter | Positive Result | Negative Result |
|---|---|---|
| CD45 Expression | Cells are of haematopoietic (blood-cell) origin; consistent with lymphoma, leukaemia, or inflammatory infiltration | Cells are not of blood-cell origin; may indicate a non-haematopoietic tumour or certain specific lymphoma subtypes |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Two important exceptions are worth knowing about:
- Classical Hodgkin lymphoma is characteristically CD45 negative. This means a negative result does not always rule out lymphoma — your doctor will consider this alongside other tests.
- Some anaplastic large-cell lymphomas may also show absent or very weak CD45 staining, even though they are of haematolymphoid origin. Additionally, IHC markers are usually ordered alongside CD45 to arrive at a complete diagnosis.
How to Maintain Healthy Levels
Because this is a diagnostic test rather than a monitoring test, there are no "target levels" to maintain. The following general wellness habits support overall immune health:
- Eat a balanced diet with plenty of fruits, vegetables, and lean proteins
- Avoid prolonged exposure to known carcinogens such as benzene and excessive pesticides
- Report persistent symptoms such as swollen lymph nodes, unexplained fatigue, or night sweats to your doctor promptly
Lupin Diagnostics IHC Marker - CD45 Test Price
The IHC marker - CD45 test is priced starting at ₹1,920 at Lupin Diagnostics. This test requires a visit to a Lupin Diagnostics centre, as home collection is not available for this test. The FFPE tissue block must be submitted in person or through your treating hospital.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1920 |
| CHENNAI | 1920 |
| HYDERABAD | 1920 |
| KOLKATA | 1920 |
| NAVI MUMBAI | 1920 |
| PUNE | 1920 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book the IHC marker - CD45 test online with Lupin Diagnostics:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time to submit your tissue block or sample.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The IHC marker - CD45 test is used to determine whether abnormal cells in a tissue sample are of blood-cell origin. It helps pathologists distinguish blood-related cancers such as lymphomas and leukaemias from other types of tumours. The result guides further testing and diagnosis.
The sample is obtained through a biopsy, where a small piece of tissue is surgically removed from the affected area. In some cases, a previously stored paraffin tissue block from an earlier biopsy may be submitted instead. The collection is performed by a healthcare professional in a clinical setting.
No fasting or medication restrictions are needed before this test. However, you should bring a detailed clinical history, including any previous biopsy reports or imaging results, as this information is required for accurate interpretation of the results.
Lupin Diagnostics delivers the IHC marker - CD45 test report within 7 days. The processing involves careful tissue preparation and microscopic examination by a qualified pathologist, which takes time to ensure accuracy.
The CD45 test confirms whether cells are of haematolymphoid origin, but it cannot distinguish between B-cell and T-cell lymphomas on its own. Your pathologist will typically order additional IHC markers alongside CD45 to classify the specific type of lymphoma.
Classical Hodgkin lymphoma is well known to be CD45 negative, meaning the absence of CD45 does not rule out all lymphomas. Certain anaplastic large-cell lymphomas may also stain negatively or weakly for CD45. This is why CD45 is always interpreted as part of a broader panel of tests.
No. The IHC marker - CD45 test requires a tissue biopsy, which must be collected in a clinical setting by a trained healthcare professional. The sample needs to be properly fixed and processed before it can be tested, so home collection is not possible for this test.
IHC Marker - CD45 Test: Booking, Price, and Results
