IHC Marker - CD43 Test: Booking, Price, and Results
About IHC Marker - CD43 Test
| Field | Value |
|---|---|
| Also Known As | CD43 Immunostain, CD43 IHC, Leukosialin IHC, Sialophorin Marker |
| Sample Type | Formalin-fixed paraffin-embedded (FFPE) tissue block |
| Fasting Required | No — this test uses tissue samples, not blood |
| Report Time | 7 Days |
| Recommended For | Adults and children of any gender suspected of haematological malignancies |
| Price | Starting at ₹1,920 |
What is an IHC Marker - CD43 Test?
The IHC marker - CD43 Test is a specialised laboratory test that detects the CD43 protein in preserved tissue samples using a method called immunohistochemistry (IHC). CD43, also known as leukosialin or sialophorin, is a cell surface protein found on T cells, myeloid cells, and certain other blood cells. Doctors order this test when investigating suspected blood-related cancers such as lymphomas or leukaemias. The sample used is a formalin-fixed paraffin-embedded (FFPE) tissue block obtained through a biopsy or surgical procedure.
What Does an IHC Marker - CD43 Test Measure?
This test identifies whether the CD43 protein is present in tissue cells and which cell types are expressing it. The findings are significant because different cell types behave differently with CD43. The key markers assessed are listed below:
| Marker | What it Shows |
|---|---|
| CD43 expression in T cells | Normal — T lymphocytes typically express CD43 |
| CD43 expression in myeloid cells | Normal — granulocytes, monocytes, and macrophages express CD43 |
| CD43 expression in B cells | Abnormal — normal B cells do not express CD43; positivity in B cells suggests a malignant process |
| Staining intensity and pattern | Helps pathologists assess the extent and distribution of CD43 expression |
Why is an IHC Marker - CD43 Test Done?
Doctors order the CD43 IHC test when tissue examination alone is not enough to determine whether a growth or cellular abnormality is cancerous. This test provides additional molecular detail to support or rule out specific diagnoses.
Common Symptoms That May Require This Test
A doctor may request this test if a patient presents with one or more of the following symptoms:
- Unexplained enlargement of lymph nodes
- Persistent fatigue without a clear cause
- Unexplained weight loss over a short period
- Night sweats occurring regularly
- Recurrent infections
- Skin lesions or unusual masses
- Abdominal swelling
Conditions This Test Can Help Detect
The IHC marker - CD43 test is used to help identify or classify the following conditions:
- T-cell lymphoma: a large proportion of T-cell malignancies express CD43
- B-cell lymphomas: such as chronic lymphocytic leukaemia (CLL), mantle cell lymphoma, and prolymphocytic leukaemia
- Myeloid sarcoma: CD43 is one of the most sensitive markers for this condition, detected in all examined cases
- Burkitt lymphoma: CD43 is positive in approximately 50% of cases
- MALT lymphoma: (mucosa-associated lymphoid tissue lymphoma) — expressed in roughly 30 to 50% of cases
- Diffuse large B-cell lymphoma (DLBCL): expressed in approximately 25% of cases
How to Prepare and What to Expect
Because this test uses tissue from a biopsy or surgical sample rather than a blood draw, preparation is different from routine blood tests. Here is what you need to know before submitting your sample.
Do You Need to Fast?
No fasting is required. This test does not involve a blood draw, so there are no dietary restrictions.
Practical Tips Before Your Test
Keep the following points in mind before bringing your sample to a Lupin Diagnostics centre:
- Bring the original FFPE tissue block from your biopsy or surgical procedure
- Attach the complete original pathology report from your initial tissue examination
- Provide all relevant clinical history, including your symptoms, previous test results, and any radiological or medical reports, as this is required for the test
- Ensure the tissue block is stored and transported at ambient temperature (18 to 28 degrees Celsius) as directed
Step-by-Step Procedure
The IHC marker - CD43 test procedure involves several stages of tissue processing. The steps below describe what happens from tissue collection to result generation:
- A medical professional collects tissue through a biopsy or surgical procedure at a clinical facility.
- The fresh tissue is fixed in formalin solution for an appropriate period to preserve its structure.
- The fixed tissue is embedded in paraffin wax to create a solid block (the FFPE tissue block).
- Thin sections are cut from the block, mounted on glass slides, and stained using standard laboratory techniques.
- Immunohistochemistry is performed on the tissue sections using a CD43 antibody to identify which cells express this protein.
- A qualified pathologist examines the stained slides under a microscope and prepares the final report.
Factors That Can Affect Accuracy
- Inadequate fixation time or improper formalin concentration during tissue processing
- Poor preservation of the tissue block before submission
- The age of the paraffin section, which can reduce immunoreactivity
- Absence of appropriate positive and negative control slides
- Quality of the tissue sample obtained at the time of biopsy
Understanding Your IHC Marker - CD43 Test Results
Results from the CD43 IHC test are reported qualitatively, meaning they describe whether the CD43 protein is present or absent, along with details about staining intensity and pattern. A pathologist reviews these findings alongside your clinical history and other test results.
| Result | Interpretation |
|---|---|
| Positive | CD43 protein detected; significance depends on the cell type staining |
| Negative | CD43 protein not detected in the tissue sample |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
How to Maintain Healthy Levels
Because the IHC marker - CD43 test is a diagnostic tool and not a routine screening test, lifestyle changes cannot influence its results. The following general points are helpful to keep in mind:
- Follow your doctor's guidance on any additional tests or clinical evaluations needed after receiving results
- Maintain regular medical follow-ups if you are undergoing investigation for a suspected haematological condition
- Keep all your medical records organised so that your healthcare team has a complete picture of your health history
Lupin Diagnostics IHC Marker - CD43 Test Price
The IHC marker - CD43 test is available at Lupin Diagnostics centres, starting at ₹1,920. This test requires a centre visit; home collection is not available because tissue samples must be obtained and submitted through a clinical or surgical setting. The city-wise pricing is listed below:
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1920 |
| CHENNAI | 1920 |
| HYDERABAD | 1920 |
| KOLKATA | 1920 |
| NAVI MUMBAI | 1920 |
| PUNE | 1920 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your IHC marker - CD43 test online at Lupin Diagnostics:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time to submit your FFPE tissue block and accompanying clinical documents.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The IHC marker - CD43 test helps pathologists detect and classify blood-related cancers, including T-cell lymphomas, certain B-cell lymphomas, myeloid sarcoma, and chronic lymphocytic leukaemia. It does this by identifying the CD43 protein in preserved tissue samples. Results are always interpreted alongside other markers and clinical findings.
This test requires tissue obtained through a biopsy or surgical procedure performed by a qualified medical professional. Patients do not provide a blood sample. The tissue is processed into a paraffin-embedded block, which is then submitted to the laboratory for analysis.
No. Because the sample is a preserved tissue block from a biopsy or surgery, it must be collected in a clinical setting. Home collection is not possible for the IHC marker - CD43 test.
Results are typically available within 7 days of the laboratory receiving the tissue specimen. The exact turnaround may vary depending on the complexity of the case and whether additional markers are being assessed simultaneously.
Your doctor may request this test if lymphoma or leukaemia is suspected. Since normal B cells do not express CD43, its presence in B cells strongly indicates an abnormal or malignant process. The test helps narrow down the specific subtype of lymphoma, which is important for treatment planning.
No. The CD43 IHC test is one part of a broader panel of immunohistochemical markers. A pathologist interprets CD43 alongside other markers such as CD20, CD3, and CD5, combined with clinical history and histological examination, before reaching a final diagnosis.
Because normal B cells do not express CD43, a positive result in B cells points towards a malignant rather than a reactive (non-cancerous) condition. This finding requires further evaluation by a qualified pathologist and should be discussed with your treating doctor for appropriate next steps.
IHC Marker - CD43 Test: Booking, Price, and Results
