IHC Marker - CD30 Test: Booking, Price, and Results
About IHC Marker - CD30 Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD30 Immunostain, CD30 IHC, Ki-1 Antigen IHC, TNFRSF8 Immunostain, Ber-H2 Marker |
| Sample Type | Formalin-fixed, paraffin-embedded (FFPE) tissue block |
| Fasting Required | No — this test is performed on tissue samples, not blood |
| Report Time | 7 Days |
| Recommended For | All genders, any age — typically ordered when lymphoma or certain cancers are suspected |
| Price | Starting at ₹1,920 |
What is an IHC Marker - CD30 Test?
The IHC Marker - CD30 test is a specialised laboratory test performed on a tissue sample to detect the presence of a protein called CD30 on cell surfaces. CD30, also known as TNFRSF8, belongs to the tumour necrosis factor receptor family and appears on certain immune cells and cancer cells. Doctors order the CD30 IHC test when they suspect lymphoma or other specific malignancies. The sample used is a formalin-fixed, paraffin-embedded (FFPE) tissue block obtained through a biopsy.
What Does an IHC Marker - CD30 Test Measure?
This test uses the immunohistochemistry (IHC) method, where specific antibodies are applied to tissue slides to detect whether CD30 protein is present. The following aspects of CD30 expression are assessed:
| Parameter | What it Indicates |
|---|---|
| CD30 Expression (Negative) | No or minimal CD30 detected; normal in healthy tissue |
| CD30 Positive, Low | Less than 10% of tumour cells staining positive |
| CD30 Positive, Moderate | 10% to 50% of tumour cells staining positive |
| CD30 Positive, High | More than 50% of tumour cells staining positive |
Results are scored on a five-tiered scale based on the percentage of CD30-positive tumour cells, ranging from score 0 (fewer than 5% positive cells) to score 4 (more than 75% positive cells). The staining pattern — membranous, cytoplasmic, or Golgi — is also noted.
Why is an IHC Marker - CD30 Test Done?
A doctor may order the IHC marker - CD30 test as part of a tissue examination when certain symptoms or clinical findings point to a possible lymphoma or related condition.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to arrange a biopsy and subsequent CD30 IHC testing:
- Painless, swollen lymph nodes in the neck, armpit, or groin
- Unexplained persistent fever
- Drenching night sweats
- Unexplained weight loss of more than 10% of body weight over six months
- Persistent fatigue without a clear cause
- Itchy skin with no obvious reason
- Shortness of breath
Conditions This Test Can Help Detect
The IHC marker - CD30 test helps identify or confirm a range of conditions. These include:
- Classic Hodgkin lymphoma (cHL), where nearly all tumour cells express CD30
- Anaplastic large cell lymphoma (ALCL), another cancer with very high CD30 expression
- Diffuse large B-cell lymphoma (DLBCL) and primary mediastinal B-cell lymphoma (PMBL)
- Peripheral T-cell lymphoma (PTCL) and mycosis fungoides
- Lymphomatoid papulosis, a recurring skin condition
- Embryonal carcinoma, malignant melanoma, and certain mesenchymal (connective tissue) tumours
- Reactive conditions such as infectious mononucleosis
How to Prepare and What to Expect
Because this test is performed on tissue, the preparation process differs from a routine blood test. The steps below will help you understand what is involved.
Do You Need to Fast?
No fasting is required. This test is carried out on a tissue sample obtained through a biopsy, not on blood or urine.
Practical Tips Before Your Test
Being well prepared helps ensure the test produces reliable results. Keep the following in mind:
- Bring a detailed clinical history, including your symptoms, previous test results, and any relevant family history, as this is required for the test
- Carry your original histopathology report and any related clinical or radiological records to assist the pathologist
- The tissue specimen must come from a malignant lymph node or extranodal tissue collected by core or excisional/incisional biopsy
- Samples from fine needle aspirates, bone marrow, skin (cutaneous), or cytology preparations are generally not acceptable
- Inform your doctor of any prior chemotherapy or radiation treatment before the biopsy, as this may affect how CD30 is expressed in the tissue
Step-by-Step Procedure
The IHC marker - CD30 test procedure involves several stages, from biopsy to final reporting:
- A surgeon or interventional specialist collects a tissue sample from the suspected lymph node or tumour site through a biopsy — this is a separate clinical procedure arranged before the IHC test.
- The collected tissue is preserved using a process called formalin fixation and paraffin embedding (FFPE), and thin slices are cut onto glass slides.
- The slides are treated with specific antibodies (typically the Ber-H2 clone) designed to bind to the CD30 protein if it is present in the tissue.
- The slides are washed, and any areas where the antibodies have bound to CD30 appear as a distinct colour when viewed under a microscope.
- A qualified pathologist examines the stained slides, assessing the percentage of CD30-positive cells, the staining intensity, and the pattern of expression.
- The FFPE tissue block is transported to the laboratory at ambient temperature (18 to 28 degrees Celsius) for processing and reporting.
Factors That Can Affect Accuracy
Several variables can influence the reliability of the IHC Marker - CD30 test:
- The quality of tissue fixation and how quickly the sample was processed after collection
- Differences in tissue processing protocols between laboratories
- The antibody clone, dilution, and staining platform used
- Prior chemotherapy or radiation, which may reduce or alter CD30 expression
- Poor tissue preservation, which can lead to false-negative results
- The experience and judgement of the reviewing pathologist
Understanding Your IHC Marker - CD30 Test Results
Results from this test are qualitative and must always be reviewed by a specialist in the context of your full clinical picture. A pathologist will report whether CD30 expression is negative or positive, along with the percentage of positive cells and the staining pattern.
| Result Category | Typical Finding |
|---|---|
| Negative | No or minimal CD30 detected (less than 1% of cells) |
| Score 0 | Fewer than 5% of tumour cells positive |
| Score 1 | 5% to 24% of tumour cells positive |
| Score 2 | 25% to 49% of tumour cells positive |
| Score 3 | 50% to 75% of tumour cells positive |
| Score 4 | More than 75% of tumour cells positive |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain factors can affect how results should be interpreted:
- CD30 staining results on FFPE samples can vary between laboratories due to differences in tissue processing and staining protocols. Interpretation should be done in the context of a validated laboratory method.
- Patients who have received chemotherapy or radiation before the biopsy may show altered CD30 expression, which could affect both diagnosis and treatment eligibility.
How to Maintain Healthy Levels
CD30 is a cancer biomarker, so there are no specific lifestyle habits that directly influence its expression. The following general guidance applies:
- Attend all follow-up appointments as recommended by your oncologist or treating doctor
- Maintain a balanced diet and adequate rest to support overall health during any ongoing treatment
- Report any new or changing symptoms to your doctor promptly so that further investigations can be arranged if needed
Lupin Diagnostics IHC Marker - CD30 Test Price
The IHC marker - CD30 test cost at Lupin Diagnostics starts at ₹1,920. This test requires a visit to a Lupin Diagnostics centre; home collection is not available, as the test is performed on a tissue biopsy sample collected in a clinical setting.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1920 |
| CHENNAI | 1920 |
| HYDERABAD | 1920 |
| KOLKATA | 1920 |
| NAVI MUMBAI | 1920 |
| PUNE | 1920 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Booking the IHC marker - CD30 test online at Lupin Diagnostics is straightforward:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample submission.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The IHC marker - CD30 test is used to detect the presence of the CD30 protein in tissue samples. It is required for patients with suspected classic Hodgkin lymphoma and peripheral T-cell lymphoma, and it also helps guide treatment decisions by identifying whether a patient may respond to CD30-targeted therapies.
No. The CD30 IHC test is performed on a tissue sample, not blood. A formalin-fixed, paraffin-embedded (FFPE) tissue block from a biopsy is the required specimen. Home sample collection is therefore not possible for this test.
A positive result indicates that CD30 protein has been detected in the tissue sample, which may support a diagnosis of certain lymphomas or other malignancies. However, the test is not conclusive on its own. Your doctor will interpret it alongside your symptoms, imaging findings, and other laboratory results.
CD30 has become an important target for a drug known as brentuximab vedotin, an antibody-drug conjugate. Patients whose tumour cells test positive for CD30 may be eligible for this targeted therapy, making the test valuable not just for diagnosis but also for selecting the most appropriate treatment.
Classic Hodgkin lymphoma and anaplastic large cell lymphoma (ALCL) consistently show high CD30 expression. Other conditions, including diffuse large B-cell lymphoma, mycosis fungoides, peripheral T-cell lymphomas, embryonal carcinoma, and malignant melanoma, may also express CD30 to varying degrees.
At Lupin Diagnostics, the IHC marker - CD30 test report is typically delivered within 7 days of the laboratory receiving the specimen. Turnaround times may vary depending on sample quality and volume at the processing centre.
Yes, in most cases. When a biopsy is collected, the tissue block can be used to run several IHC markers, including CD30, along with other relevant markers. Your doctor or pathologist will advise which panel of markers is appropriate based on your clinical presentation.
IHC Marker - CD30 Test: Booking, Price, and Results
