IHC Marker - CD25 Test: Booking, Price, and Results
About IHC Marker - CD25 Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD25 IHC, IL-2 Receptor Alpha Chain IHC, IL-2Rα IHC, Interleukin-2 Receptor IHC, CD25 Immunostain |
| Sample Type | Formalin-fixed, paraffin-embedded (FFPE) tissue block |
| Fasting Required | No — no dietary restrictions or special preparation needed |
| Report Time | 3 days |
| Recommended For | All genders; primarily adults with suspected blood cancers or lymphomas |
| Price | Starting at ₹1,920 |
What is an IHC Marker - CD25 Test?
The CD25 test is a specialised laboratory test that detects the presence of a protein called CD25 in tissue samples. CD25, also known as the IL-2 receptor alpha chain, appears on the surface of certain immune cells and is often overexpressed in blood cancers and lymphomas. A doctor typically orders this test after a biopsy to help identify the type of cancer affecting a patient's lymph nodes, blood, or bone marrow. The test is also referred to as CD25 IHC or the CD25 Immunostain.
What Does an IHC Marker - CD25 Test Measure?
This test uses a technique called immunohistochemistry (IHC) to identify specific proteins within tissue. IHC works by applying targeted antibodies to a tissue sample; these antibodies bind to the CD25 protein, making it visible under a microscope.
The key elements examined in this test are listed below.
| Component | What It Tells Us |
|---|---|
| CD25 Protein Expression | Whether the CD25 protein is present in the tissue cells |
| Staining Pattern | Where and how intensely the protein appears within the tissue |
| Cell Type Involvement | Which type of immune cells (T cells, B cells, or mast cells) are affected |
A pathologist studies the stained tissue slides and reports the result as positive or negative, along with the intensity and distribution of staining.
Why is an IHC Marker - CD25 Test Done?
A doctor may request this test when clinical findings suggest a possible blood cancer or lymphoma. Here is a closer look at the situations in which it is used.
Common Symptoms That May Require This Test
Several symptoms may prompt a doctor to investigate further with a CD25 IHC test. These include:
- Unexplained weight loss
- Recurring fevers
- Heavy night sweats
- Persistent fatigue
- Easy bruising or bleeding
- Frequent infections
- Swollen lymph nodes (lymphadenopathy)
- Fullness or discomfort in the left side of the abdomen due to an enlarged spleen (splenomegaly)
- Reddish or brown skin patches
- Severe itching (pruritus)
- Flushing
- Skin rashes
Conditions This Test Can Help Detect
The CD25 test assists pathologists in identifying a range of blood-related conditions, including:
- Hairy cell leukaemia, a type of B-cell lymphoma where CD25 is strongly expressed
- Adult T-cell leukaemia/lymphoma (ATLL), where CD25 is characteristically positive
- Classical Hodgkin lymphoma, where CD25 appears in Reed-Sternberg cells
- Peripheral T-cell lymphomas, a subset of which express CD25
- Systemic mastocytosis, where CD25 helps distinguish cancerous mast cells from normal ones
How to Prepare and What to Expect
The CD25 test procedure is performed on tissue that has already been collected by your doctor. Here is what to know before and during the process.
Do You Need to Fast?
No fasting is required. There are no dietary restrictions or fluid limitations before this test.
Practical Tips Before Your Test
A few simple steps will help ensure your sample is ready and the process goes smoothly. Keep the following in mind:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Bring all previous pathology reports and imaging results to your appointment
- Inform your doctor about any medications you are taking, especially blood thinners, before the biopsy procedure
- You do not need to visit the laboratory separately — the IHC test is performed on the tissue block already collected during your biopsy
Step-by-Step Procedure
The CD25 test procedure involves several steps carried out in the laboratory after your biopsy. Here is what happens:
- Your doctor collects a small piece of tissue through a biopsy, then preserves it in formalin to maintain the structure of the cells and proteins.
- Laboratory technicians embed the preserved tissue in paraffin wax to create a firm block, which is then cut into extremely thin slices (around 4 to 5 microns) and placed on glass slides.
- The slides are treated with heat or enzymes in a step called antigen retrieval, which exposes proteins that may have been masked during fixation.
- Specific antibodies targeting the CD25 protein are applied to the tissue sections and left to bind to their target.
- Secondary antibodies linked to detection enzymes are applied, producing a visible colour change wherever CD25 is present.
- A qualified pathologist examines the stained slides under a microscope and prepares a detailed report.
Factors That Can Affect Accuracy
Certain factors may influence how reliably the test detects the CD25 protein. These include:
- Poor tissue fixation, such as delays between biopsy and preservation
- Previous chemotherapy or radiation therapy, which can alter protein expression in tissue
- Very small tissue samples, which may limit the material available for analysis
- The age of the tissue sample and how it was stored before processing
Understanding Your IHC Marker - CD25 Test Results
Results from this test require careful interpretation by a trained pathologist in the context of your full clinical picture. The table below outlines general guidelines.
| Result | Typical Interpretation |
|---|---|
| Negative (scattered positive cells only) | CD25 protein not detected in tumour cells; consistent with normal tissue or reactive immune response |
| Positive | CD25 protein detected in tumour cells; may indicate hairy cell leukaemia, ATLL, Hodgkin lymphoma, or systemic mastocytosis |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain situations can affect how results are interpreted.
- Previous chemotherapy or radiation therapy before the biopsy may reduce or alter CD25 expression in the tissue, which could affect the test outcome.
- Delays between biopsy collection and tissue fixation can also degrade protein structure, potentially impacting detection accuracy.
How to Maintain Healthy Levels
While there is no specific way to control CD25 expression, general habits that support immune health are worthwhile.
- Eat a balanced diet with adequate vitamins and minerals to support immune function.
- Attend regular medical check-ups, particularly if you have a personal or family history of blood cancers.
- Avoid known carcinogens and unnecessary exposure to radiation where possible.
Lupin Diagnostics IHC Marker - CD25 Test Price
The CD25 test cost at Lupin Diagnostics starts at ₹1,920. This test requires a visit to a Lupin Diagnostics centre; home collection is not available, as it is performed on tissue blocks obtained through a biopsy procedure.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1920 |
| CHENNAI | 1920 |
| HYDERABAD | 1920 |
| KOLKATA | 1920 |
| NAVI MUMBAI | 1920 |
| PUNE | 1920 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Booking your CD25 test online at Lupin Diagnostics is straightforward. Follow these steps:
- Select the IHC Marker CD25 test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample submission or to coordinate tissue block transfer.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The CD25 IHC test identifies the presence of the CD25 protein in tissue samples. This protein is frequently overexpressed in certain blood cancers and lymphomas, making it a useful marker to help pathologists classify the type of disease present.
The tissue sample is collected through a biopsy, a procedure carried out by your doctor. Once the tissue block is prepared, you do not need to visit the laboratory separately; the IHC analysis is performed on the block already collected.
No fasting is required. There are no dietary or fluid restrictions before this test.
At Lupin Diagnostics, the report is typically ready within 3 days of the laboratory receiving the tissue block. Turnaround times may vary slightly depending on the complexity of the case.
A positive result means the CD25 protein was detected in the tissue sample. This finding may point to conditions such as hairy cell leukaemia, adult T-cell leukaemia/lymphoma, or systemic mastocytosis. A positive result alone does not confirm a diagnosis; your doctor will consider it alongside other clinical and laboratory findings.
A negative result indicates that the CD25 protein was not detected in the tissue. This can help rule out certain lymphomas and leukaemias, such as classic hairy cell leukaemia. Your doctor will explain what the result means in the context of your overall health.
No, home collection is not available for this test. Because it requires a tissue biopsy and a specially prepared tissue block, sample collection and submission must take place at a healthcare centre or Lupin Diagnostics facility.
IHC Marker - CD25 Test: Booking, Price, and Results
