IHC Marker-CD15 Test: Booking, Price, and Results
About IHC Marker-CD15 Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD15 Immunohistochemistry, IHC Leu M1, CD15 Immunostain, Lewis X Antigen, X-Hapten Marker |
| Sample Type | Formalin-fixed, paraffin-embedded (FFPE) tissue block |
| Fasting Required | No fasting required |
| Report Time | 7 days |
| Recommended For | Male and female, all ages |
| Price | Starting at ₹1,920 |
What is an IHC Marker-CD15 Test?
The IHC Marker-CD15 test is a specialised laboratory test that detects the presence of the CD15 protein in tissue samples using immunohistochemistry. CD15, also known as Leu-M1 or the Lewis X antigen, is a marker found on certain white blood cells and on some cancer cells. Doctors order this test to help identify or rule out specific cancers, particularly lymphomas and leukaemias. The sample is a formalin-fixed, paraffin-embedded (FFPE) tissue block obtained by biopsy.
What Does an IHC Marker-CD15 Test Measure?
This test analyses the expression of the CD15 protein in tissue. The findings help a pathologist classify a disease and guide clinical decisions. The test examines the following:
| Component | What it Indicates |
|---|---|
| CD15 expression in myeloid cells | Normal findings in granulocytes such as neutrophils and eosinophils |
| CD15 in Reed-Sternberg cells | When positive alongside CD30, suggests classical Hodgkin lymphoma |
| CD15 in suspected mesothelioma | A negative result helps distinguish mesothelioma from adenocarcinoma |
| Lewis X (LeX) antigen activity | Reflects the role of CD15 in cell interactions and immune functions |
Why is an IHC Marker-CD15 Test Done?
A doctor may request this test when tissue analysis is needed to confirm or rule out certain cancers. Below are the common reasons it is ordered.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to request a biopsy and subsequent IHC CD15 testing:
- Unexplained swollen lymph nodes in the neck, armpits, or groin
- Persistent fever without a clear cause
- Drenching night sweats
- Unexplained weight loss
- Unusual fatigue or weakness
- Generalised itching
- Recurrent infections
Conditions This Test Can Help Detect
The IHC Marker-CD15 test is used to help identify or differentiate the following conditions:
- Classical Hodgkin lymphoma, where Reed-Sternberg cells are typically positive for both CD15 and CD30 in around 80% of cases
- Acute myeloid leukaemia (AML), a cancer of the blood and bone marrow, where CD15 helps classify certain subtypes
- Chronic myeloid leukaemia (CML), which regularly shows CD15 positivity
- Distinction between epithelial mesothelioma and adenocarcinoma, where a negative CD15 result supports a mesothelioma diagnosis
How to Prepare and What to Expect
Because this test uses a tissue sample obtained via biopsy, preparation is straightforward. Your doctor or surgical team will give you specific biopsy instructions based on the type of procedure and its location.
Do You Need to Fast?
No fasting is required for the IHC Marker-CD15 test itself. However, if your biopsy requires sedation or general anaesthesia, your doctor may ask you to fast beforehand. Follow any pre-biopsy instructions provided by your healthcare team.
Practical Tips Before Your Test
Here are a few practical steps to keep in mind before your biopsy and testing:
- Bring a detailed clinical history, including your symptoms, previous test results, and relevant medical records, as this is required for the test
- Inform your doctor about all medications you are taking, especially blood thinners or anticoagulants
- Follow any specific pre-biopsy preparation instructions from your doctor regarding food, drink, or medication
- Wear comfortable, loose clothing to your biopsy appointment
Step-by-Step Procedure
The IHC Marker-CD15 test procedure involves several stages, from tissue collection to final analysis:
- Your doctor arranges a biopsy to obtain a small tissue sample from the area of concern, such as a lymph node or tumour mass.
- The tissue is preserved in formalin and then embedded in paraffin wax to create an FFPE tissue block, which is submitted to the laboratory.
- In the lab, thin sections of the tissue are cut and placed on microscope slides.
- The slides are treated with antibodies that specifically target the CD15 protein. If CD15 is present, it binds to the antibodies and produces a visible colour signal.
- A qualified pathologist examines the stained slides under a microscope and records whether CD15 staining is positive, negative, or focal.
- The results are interpreted alongside your clinical history and other diagnostic findings, and a report is issued within 7 days.
Factors That Can Affect Accuracy
The following technical factors can influence the quality and reliability of IHC staining results:
- Quality and duration of tissue fixation in formalin before processing
- Proper handling and storage of the tissue block during transport
- The age of the paraffin-embedded sections used for staining
- Antibody quality and the antigen retrieval technique used in the laboratory
- Inadequate clinical history was submitted with the sample
Understanding Your IHC Marker-CD15 Test Results
Results from this test are always interpreted by a pathologist alongside your full clinical picture. The table below summarises typical findings.
| Parameter | Finding | Interpretation |
|---|---|---|
| CD15 in granulocytes | Positive | Normal expression in myeloid lineage cells |
| CD15 in Reed-Sternberg cells | Positive (with CD30) | Consistent with classical Hodgkin lymphoma |
| CD15 in suspected mesothelioma | Negative | Supports mesothelioma diagnosis over adenocarcinoma |
| CD15 in nodular lymphocyte predominance Hodgkin lymphoma | Negative | Helps distinguish from classical Hodgkin lymphoma |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
How to Maintain Healthy Levels
CD15 expression reflects an underlying disease process and is not directly modified by lifestyle. However, the following general steps support your overall health:
- Attend regular health check-ups and report any unusual lumps, swellings, or persistent symptoms to your doctor promptly
- Follow your doctor's advice regarding any recommended follow-up tests or imaging studies
- Maintain a balanced diet, regular physical activity, and adequate rest to support your immune health generally
Lupin Diagnostics IHC Marker-CD15 Test Price
The IHC Marker-CD15 test is available at Lupin Diagnostics starting at ₹1,920. This test requires a visit to a Lupin Diagnostics centre; home collection is not available because the tissue sample must be obtained through a biopsy performed by a medical professional in a clinical setting.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1920 |
| CHENNAI | 1920 |
| HYDERABAD | 1920 |
| KOLKATA | 1920 |
| NAVI MUMBAI | 1920 |
| PUNE | 1920 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your IHC Marker-CD15 test online:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time to collect your sample or submit your FFPE tissue block.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The IHC Marker-CD15 test detects the presence of the CD15 protein in tissue samples. It helps pathologists identify or rule out conditions such as classical Hodgkin lymphoma, acute myeloid leukaemia, and certain other cancers. It also helps distinguish mesothelioma from adenocarcinoma when examining pleural tissue.
No fasting is needed for the IHC Marker-CD15 test itself, as it is performed on a tissue sample rather than blood or urine. If your biopsy involves sedation or anaesthesia, your doctor will give you separate fasting instructions for that procedure.
The tissue sample is obtained through a biopsy, which your doctor arranges based on the location of the suspected abnormality. The collected tissue is fixed in formalin and embedded in paraffin wax to create an FFPE block, which is then sent to the laboratory for IHC CD15 analysis.
A positive CD15 result in lymph node tissue may suggest classical Hodgkin lymphoma, particularly when CD30 is also positive. In blood-related cancers, it may point to acute or chronic myeloid leukaemia. A positive result alone is not a diagnosis; your doctor will review it alongside imaging, other markers, and your clinical history.
No. CD15 is positive in approximately 80% of classical Hodgkin lymphoma cases. In nodular lymphocyte predominance Hodgkin lymphoma, the characteristic popcorn cells are usually negative for CD15. This is why CD15 is used as part of a broader panel of markers, not in isolation.
At Lupin Diagnostics, the report is delivered within 7 days. This allows adequate time for tissue processing, staining, and pathologist review. The report is made available digitally via email or WhatsApp.
CD15 staining results must be interpreted in context. Providing accurate clinical information, including symptoms, previous diagnoses, and other test results, allows the pathologist to make a more precise and relevant interpretation. Submitting an incomplete clinical history may affect the quality of the final report.
IHC Marker-CD15 Test: Booking, Price, and Results
