IHC Marker-CD10 Test: Booking, Price, and Results
About IHC Marker-CD10 Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD10 IHC, CALLA (Common Acute Lymphoblastic Leukaemia Antigen), Neprilysin, Neutral Endopeptidase (NEP), MME (Membrane Metalloendopeptidase) |
| Sample Type | FFPE Tissue Block (formalin-fixed, paraffin-embedded tissue) |
| Fasting Required | No |
| Report Time | 7 Days |
| Recommended For | All genders and ages, as ordered by a pathologist or oncologist |
| Price | Starting at ₹1,920 |
What is an IHC Marker-CD10 Test?
The IHC marker-CD10 test is a specialised laboratory test that detects the presence of a protein called CD10 in tissue samples. CD10, also known as CALLA or neprilysin, is found on the surface of certain immune and kidney cells. Doctors use this test to help classify tumours and diagnose specific cancers. The sample is a tissue block collected during a biopsy procedure and processed using a technique called immunohistochemistry (IHC).
What Does an IHC Marker-CD10 Test Measure?
The IHC marker-CD10 test examines tissue sections to determine whether CD10 protein is present and how it is expressed. A pathologist assesses three key aspects of this expression.
| Parameter | What is Assessed |
|---|---|
| CD10 Expression | Whether the marker is positive (present) or negative (absent) |
| Staining Intensity | How strongly the cells react: weak, moderate, or strong |
| Distribution | Whether positive cells are scattered (focal) or widespread (diffuse) |
CD10 is a zinc-dependent enzyme on the cell surface that breaks down small protein fragments. Its presence in certain tissues helps doctors identify the origin and type of a tumour.
Why is an IHC Marker-CD10 Test Done?
A pathologist or oncologist may request the IHC marker-CD10 test when a biopsy result needs further classification. Here is when this test is typically used.
Common Symptoms That May Require This Test
The following symptoms may lead a doctor to request a tissue biopsy and subsequent IHC testing:
- Unexplained swelling of lymph nodes
- Persistent fatigue without a clear cause
- Unexplained weight loss
- Night sweats
- Abnormal masses or lumps detected on imaging
- Suspected tumour identified after initial biopsy
Conditions This Test Can Help Detect
The CD10 IHC test assists in the diagnosis and classification of several conditions. These include:
- B-precursor acute lymphoblastic leukaemia (a blood cancer affecting immature immune cells)
- Burkitt lymphoma (an aggressive B-cell lymphoma)
- Follicular lymphoma and related lymphomas of follicle centre cell origin
- Renal cell carcinoma (kidney cancer)
- Endometrial stromal sarcoma (a rare uterine cancer)
- Breast lesion differentiation, including identification of myoepithelial cells
How to Prepare and What to Expect
Because this test is performed on tissue already collected during a biopsy, patient preparation focuses primarily on the biopsy procedure and on providing accurate information to your doctor.
Do You Need to Fast?
No fasting is required for this test. The IHC marker-CD10 test procedure is carried out on a tissue block in the laboratory, not on a fresh blood or urine sample collected from you on the day of testing.
Practical Tips Before Your Test
Keep the following in mind before your sample is submitted to the laboratory:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test.
- Ensure that your tissue block or slides are correctly labelled before submission.
- Share all previous biopsy reports and imaging results with your doctor.
- Inform your doctor about any medications you are taking or any underlying medical conditions.
Step-by-Step Procedure
The following steps describe how the IHC marker-CD10 test is performed in the laboratory:
- Tissue collected during a biopsy is fixed in formalin, a chemical that preserves the sample's structure.
- The fixed tissue is embedded in paraffin wax to allow precise slicing.
- Thin sections (approximately 4 to 5 micrometres) are cut using a specialised slicing instrument and placed on glass slides.
- A CD10-specific antibody is applied to the tissue section. This antibody binds only to the CD10 protein where it is present.
- A coloured staining agent is added so that the bound antibody becomes visible under a microscope.
- A qualified pathologist examines the slides and records the pattern, intensity, and distribution of staining.
Factors That Can Affect Accuracy
Several factors may influence the quality and reliability of results:
- Improper or prolonged tissue fixation, which can mask target proteins
- Errors in paraffin embedding or slide preparation
- Quality and concentration of the antibodies used in the laboratory
- Absence of appropriate positive and negative control slides
- An incomplete or missing clinical history submitted with the sample
Understanding Your IHC Marker-CD10 Test Results
Results from the IHC marker-CD10 test are qualitative and must be interpreted by a qualified pathologist alongside the patient's clinical history and other test findings.
| Parameter | Positive | Negative |
|---|---|---|
| CD10 Expression | CD10 protein detected in cells | CD10 protein absent in cells |
| Clinical Significance | May indicate B-cell leukaemia, follicular lymphoma, kidney cancer, or endometrial stromal sarcoma | May suggest non-follicular centre origin or require additional IHC markers |
A positive CD10 result may be associated with acute lymphoblastic leukaemia, Burkitt lymphoma, renal cell carcinoma, and endometrial stromal sarcoma, among other conditions. A negative result does not always rule out malignancy; additional markers are often needed for a definitive diagnosis.
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
How to Maintain Healthy Levels
While CD10 expression cannot be managed through lifestyle changes, you can support your overall health in the following ways:
- Attend regular health check-ups as recommended by your doctor.
- Report persistent or unexplained symptoms such as fatigue, lymph node swelling, or weight loss promptly.
- Follow your oncologist's guidance if you are currently undergoing cancer evaluation or treatment.
Lupin Diagnostics IHC Marker-CD10 Test Price
The IHC marker-CD10 test cost at Lupin Diagnostics starts at ₹1,920. This test requires a visit to a Lupin Diagnostics centre; home collection is not available as the sample is a tissue block obtained through a biopsy procedure.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1920 |
| CHENNAI | 1920 |
| HYDERABAD | 1920 |
| KOLKATA | 1920 |
| NAVI MUMBAI | 1920 |
| PUNE | 1920 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
You can book the IHC marker-CD10 test online by following these steps:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample submission.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The IHC marker-CD10 test helps diagnose and classify certain cancers. It is most commonly used to identify B-precursor acute lymphoblastic leukaemia, Burkitt lymphoma, follicular lymphoma, kidney cancer, and endometrial stromal sarcoma. A pathologist uses the results alongside other markers to reach a definitive diagnosis.
The sample is a tissue block collected during a biopsy procedure at a hospital or surgical facility. The tissue is fixed in formalin and embedded in paraffin wax before being sent to the laboratory. No separate sample collection is needed once the biopsy has been performed.
No. The IHC marker-CD10 test home collection option is not available because the sample is a tissue block that must be obtained through a clinical biopsy procedure, not a standard blood or urine draw.
The report is typically delivered within 7 days of the sample reaching the laboratory. Turnaround time may vary depending on the complexity of the case and any additional markers required.
A positive result means CD10 protein was detected in the tissue sample. This may be associated with certain lymphomas, leukaemias, kidney cancers, or other conditions. However, a positive result alone is not a diagnosis. Your pathologist will interpret it alongside your clinical history and other test findings.
No. CD10 is also present in several normal tissues, including the lining of the intestinal tract, kidney cells, liver bile channels, lung cells, breast myoepithelial cells, and endometrial stromal cells. This is why the pathologist reviews staining patterns carefully and considers the full clinical picture.
The IHC marker-CD10 test is primarily a diagnostic tool used to classify tumours at the time of initial evaluation. It can help determine the grade of certain lymphomas, which informs treatment decisions, but it is not routinely used for ongoing treatment monitoring.
IHC Marker-CD10 Test: Booking, Price, and Results
