IHC Marker - Any 7 Markers Test: Booking, Price, and Results
About IHC Marker - Any 7 Markers Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | IHC Panel (7 Markers), Immunohistochemistry 7 Markers, IHC 7-Marker Panel, Immunohistochemical Staining Panel |
| Sample Type | FFPE tissue block (formalin-fixed, paraffin-embedded biopsy specimen) |
| Fasting Required | No. This test is performed on tissue, not blood |
| Report Time | 4 days |
| Recommended For | All genders and ages, as directed by an oncologist or pathologist |
| Price | Starting at ₹8,000 |
What is an IHC Marker - Any 7 Markers Test?
The IHC marker - Any 7 Markers test is a specialised laboratory test that examines tissue samples for specific proteins. The method, known as immunohistochemistry, uses antibodies to detect whether particular proteins are present in the cells of a biopsy sample. Doctors use this test primarily to diagnose and characterise cancers. It is also referred to as an IHC 7-Marker Panel or Immunohistochemistry 7 Markers test.
What Does an IHC Marker - Any 7 Markers Test Measure?
This panel examines up to seven protein markers chosen by your treating doctor based on your clinical situation. The specific markers tested vary by case but commonly include the following.
| Marker | What It Indicates |
|---|---|
| ER (Oestrogen Receptor) | Hormone receptor status, relevant in breast cancer |
| PR (Progesterone Receptor) | Hormone sensitivity of tumour cells |
| HER2 | Identifies patients who may benefit from targeted therapies |
| Ki-67 | Measures how rapidly tumour cells are dividing |
| TTF-1 | Helps diagnose primary lung adenocarcinoma |
| Cytokeratins (CK) | Used to identify carcinomas and confirm tissue origin |
| CD Markers (e.g., CD15, CD30) | Used in diagnosing lymphomas and related conditions |
Why is an IHC Marker - Any 7 Markers Test Done?
This test is ordered when a doctor needs detailed information about a tissue sample, usually after a biopsy. It helps confirm a diagnosis and guides decisions about treatment.
Common Symptoms That May Require This Test
Your doctor may recommend the IHC marker - Any 7 Markers test if you present with one or more of the following:
- An unexplained lump or mass in any part of the body
- Abnormal findings on a previous biopsy report
- Suspected malignancy based on imaging or clinical examination
- Cancer of unknown primary origin that has spread to other areas
- Recurrent cancer requiring re-evaluation
- Need for detailed tumour characterisation before starting treatment
Conditions This Test Can Help Detect
The IHC 7-Marker Panel assists in identifying and classifying a range of conditions. These include the following.
- Breast cancer (hormone receptor and HER2 status)
- Lung cancer subtypes, such as adenocarcinoma and squamous cell carcinoma
- Lymphomas, including Hodgkin's disease and other subtypes
- Prostate and stomach cancers
- Metastatic tumours of uncertain origin
- Poorly differentiated tumours requiring subtyping
How to Prepare and What to Expect
Because this test uses a tissue sample rather than blood, the preparation is different from routine laboratory tests. Here is what you need to know before your test.
Do You Need to Fast?
No fasting is required. This test is performed on a tissue biopsy specimen, so food and drink do not affect the result.
Practical Tips Before Your Test
Keep the following points in mind before submitting your sample:
- Bring a detailed clinical history including your symptoms, previous test results, and family history, as this is required for the test
- Carry any prior histopathology or pathology reports to your appointment
- Inform your doctor if you have received chemotherapy or radiation therapy before the biopsy, as these treatments can alter protein expression in tissue
- Follow all instructions given by your doctor or the collecting team regarding the biopsy procedure itself
Step-by-Step Procedure
The IHC marker test procedure involves several stages in the laboratory after the biopsy is collected. Here is what happens at each step:
- A doctor collects a small tissue sample through a biopsy. This may be a needle biopsy or a surgical excision, depending on the location and clinical need.
- The tissue is preserved in formalin to maintain its cellular structure and protect the proteins within it. The sample is then embedded in paraffin wax to create a firm block (FFPE block).
- Laboratory technicians cut very thin slices of the tissue block and place them onto glass slides for staining.
- A process called antigen retrieval is carried out. This uses heat or enzymes to expose proteins that may have been masked during preservation.
- Specific antibodies are applied to the tissue. Each antibody binds to its target protein, and a chemical reaction produces a visible colour change in areas where the protein is present.
- A qualified pathologist examines the stained slides under a microscope and records the staining pattern and intensity for each of the seven markers.
Factors That Can Affect Accuracy
Several factors can influence the reliability of results. Your pathology team will take steps to minimise these, but it is useful to be aware of them:
- How quickly the tissue was placed in formalin after collection
- The duration of formalin fixation (too short or too long can affect results)
- The age of the tissue block and the time elapsed since sections were cut
- Previous cancer treatments such as chemotherapy or radiation, which can modify protein expression in the tissue
- The quality of the antibodies used and adherence to laboratory quality control protocols
Understanding Your IHC Marker - Any 7 Markers Test Results
Your results will be reviewed by a qualified pathologist and shared with your treating oncologist. The table below gives a general guide to how common markers are interpreted.
| Marker | Result | Typical Interpretation |
|---|---|---|
| ER / PR | Positive (1% or more nuclear staining) | Tumour cells are hormone-sensitive |
| ER / PR | Negative (less than 1% staining) | Tumour cells are not hormone-sensitive |
| HER2 | 3+ (Positive) | High HER2 protein expression; may be eligible for targeted therapy |
| HER2 | 2+ (Equivocal) | Further testing (FISH or ISH) is usually recommended |
| HER2 | 0 to 1+ (Negative) | No significant HER2 overexpression |
| Ki-67 | Above 20% | Rapidly dividing cells; suggests more aggressive tumour behaviour |
| Ki-67 | Below 20% | Slower cell division rate |
| Other Markers | Positive or Negative | Interpreted based on specific staining patterns per marker |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain situations can influence how results are read:
- Tissue that is not placed in fixative promptly after collection may show reduced staining intensity, which can affect accuracy.
- Specimens left for more than six hours before fixation are particularly at risk.
- If you have received chemotherapy or radiation before the biopsy, protein expression in the tissue may have changed. Your pathologist will factor this in when interpreting results.
How to Maintain Healthy Levels
Because this test is used for cancer diagnosis rather than routine monitoring, the following tips focus on general health awareness.
- Attend regular health check-ups as advised by your doctor and follow cancer screening guidelines suitable for your age and risk profile.
- Report any new lumps, unexplained weight loss, persistent fatigue, or other unusual symptoms to your doctor without delay.
- Follow up with your oncologist after receiving results to understand the next steps in your care.
Lupin Diagnostics IHC Marker - Any 7 Markers Test Price
The IHC Marker - Any 7 Markers test cost at Lupin Diagnostics starts at ₹8,000. This test requires a visit to a Lupin Diagnostics centre or affiliated collection point; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 8000 |
| CHENNAI | 8000 |
| HYDERABAD | 8000 |
| KOLKATA | 8000 |
| NAVI MUMBAI | 8000 |
| PUNE | 8000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Use the steps below for IHC marker - Any 7 Markers test online booking:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample submission.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
An IHC marker test, or immunohistochemistry test, uses antibodies to detect specific proteins in a tissue sample. It is widely used in cancer pathology to identify what type of tumour is present, where it originated, and which treatments may be effective.
A panel of seven markers gives a fuller picture of a tumour than a single marker alone. When interpreted alongside your clinical findings and medical history, the IHC 7-Marker Panel helps doctors reach an accurate diagnosis and plan the most appropriate treatment.
The tissue is collected through a biopsy performed by a doctor at a hospital or clinic. Depending on the location of the tissue, this may involve a fine needle, a core needle, or a surgical procedure. Your doctor will explain the specific method recommended for your case.
Results are typically available within 4 days at Lupin Diagnostics. Complex panels may occasionally take longer. Your doctor will advise you on the expected timeline.
No, the IHC marker - Any 7 Markers test home collection is not available. This test requires a tissue biopsy performed by a trained medical professional at a hospital or clinic. The FFPE tissue block is then submitted to the Lupin Diagnostics laboratory for analysis.
Yes. If cancer recurs or spreads to a new site, the tumour's protein profile may differ from the original. Doctors may recommend repeat IHC testing in such cases to ensure that treatment decisions are based on the most current tumour characteristics.
The accuracy of this test depends on several factors, including how quickly the biopsy tissue was fixed after collection, the duration of fixation, the quality of antibodies used, and the experience of the pathologist. Prior chemotherapy or radiation can also affect protein expression in the tissue sample.
IHC Marker - Any 7 Markers Test: Booking, Price, and Results
