IHC Marker-Any 10 Markers Test: Booking, Price, and Results

The IHC marker-Any 10 Markers test is a specialised laboratory test that uses antibodies to detect specific proteins within a tissue sample. It helps pathologists identify the type, origin, and behaviour of abnormal cells, including cancerous ones. The test is performed on tissue collected through a biopsy or surgical procedure. It is also referred to as the IHC 10 Marker Panel or Immunohistochemistry Extended Panel.

A doctor may order this test when imaging results or a biopsy raises concern about cancer. The following subheadings explain the common reasons in more detail.

Common Symptoms That May Require This Test

A doctor may recommend this test when a patient presents with any of the following:

• A new lump or mass found during a physical examination
• Abnormal findings on imaging tests such as mammograms, CT scans, or MRIs
• Persistent, unexplained pain or discomfort in a specific body area
• Unexplained weight loss alongside other concerning symptoms
• Swollen lymph nodes that do not resolve on their own
• Unusual bleeding or changes in an existing growth or lesion

Conditions This Test Can Help Detect

The IHC marker-Any 10 Markers test procedure can assist in identifying a range of conditions, including:

• Breast cancer subtypes, including hormone receptor status and HER2 expression
• Lymphomas and other blood cancers requiring classification for therapy selection
• Sarcomas, including specific subtypes of soft tissue and bone cancers
• Cancer of unknown primary, where the original site of a metastatic tumour is unclear
• Certain infectious diseases and immune system conditions

The IHC Marker-Any 10 Markers test procedure follows these main steps:

1. A tissue sample is obtained from the patient through a biopsy or surgical procedure performed by the treating doctor.
2. The tissue is preserved using formalin and then embedded in paraffin wax to create a firm block (the FFPE block). This keeps the tissue intact for analysis.
3. Very thin slices of the tissue block are cut and placed on microscope slides in the laboratory.
4. Each slide is treated with specific antibodies that attach to target proteins within the cells.
5. A coloured dye is activated wherever an antibody binds to its matching protein, making the target visible under a microscope.
6. A trained pathologist examines the stained slides and interprets the pattern of protein expression to prepare a detailed report.

IHC results do not follow a traditional numerical reference range. Instead, results are reported based on the presence, absence, or intensity of specific protein markers. A pathologist reviews all findings in the context of your clinical history.

Below is a general guide to how common markers are reported:

These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.

Results During Special Conditions

Certain conditions related to sample handling can influence result accuracy:

• Over-fixation causes excessive protein cross-linking, which can mask the target proteins and reduce antibody binding, leading to weaker or absent staining.
• Under-fixation may result in uneven staining and poor tissue preservation, making interpretation more difficult.
• A delay of even 24 hours before fixation begins can reduce the intensity of staining, which is why timely processing of the biopsy sample is essential.

The IHC marker-Any 10 Markers test cost at Lupin Diagnostics starts at ₹9,500. This test requires a visit to a Lupin Diagnostics centre, as home collection is not available for tissue-based tests of this nature.

Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.