FLT3 (ITD, D835Y) Mutation Detection Test: Booking, Price, and Results
About FLT3 (ITD, D835Y) Mutation Detection Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | FLT3 Gene Mutation Test, FLT3-ITD Mutation Analysis, FLT3-TKD Mutation Test, FMS-like Tyrosine Kinase 3 Mutation Test |
| Sample Type | Peripheral blood (EDTA tube) or bone marrow aspirate (sodium heparin tube) |
| Fasting Required | No fasting required |
| Report Time | 15 days |
| Recommended For | Adults and children diagnosed with or suspected of having acute myeloid leukaemia (AML) |
| Price | Starting at ₹5,400 |
What is a FLT3 (ITD, D835Y) Mutation Detection Test?
The FLT3 Mutation Detection Test is a specialised genetic test that identifies specific mutations in the FLT3 gene, which plays a key role in the development of blood cells. It is commonly ordered for patients diagnosed with or suspected of having acute myeloid leukaemia (AML), a type of blood cancer. The test uses a method called fragment analysis on either a peripheral blood sample or a bone marrow aspirate. It is also known as the FLT3-ITD Mutation Analysis or FMS-like Tyrosine Kinase 3 Mutation Test.
What Does a FLT3 (ITD, D835Y) Mutation Detection Test Measure?
This test detects two distinct types of genetic changes in the FLT3 gene. The table below summarises each component.
| Component | What It Looks For |
|---|---|
| FLT3-ITD Mutation | A duplication of genetic material within the juxtamembrane domain that causes the FLT3 protein to remain permanently switched on, driving uncontrolled cell growth |
| FLT3-D835Y Mutation (TKD) | A point mutation in the tyrosine kinase domain of the FLT3 gene, D835Y, is the most common substitution and is found in roughly 8% of AML patients |
| ITD Allelic Ratio | If the ITD mutation is detected, this ratio measures the proportion of mutant gene copies to normal ones; a higher ratio may indicate a worse prognosis |
Why is a FLT3 (ITD, D835Y) Mutation Detection Test Done?
This test is a key tool in the evaluation and management of acute myeloid leukaemia. It helps doctors understand the genetic profile of the disease and plan the most appropriate course of care.
Common Symptoms That May Require This Test
A doctor may order this test when a patient presents with symptoms that suggest a blood disorder. These symptoms include:
- Persistent fatigue and weakness without an obvious cause
- Unexplained fever or frequent infections
- Easy bruising or unusual bleeding, including tiny red spots on the skin (petechiae)
- Unintended weight loss
- Night sweats
- Bone pain or joint discomfort
- Shortness of breath
- Swollen lymph nodes
Conditions This Test Can Help Detect
This test is used in the context of several specific clinical situations. These include:
- Acute myeloid leukaemia (AML), a cancer of myeloid blood cells
- Risk stratification in AML, helping doctors assess how aggressive the disease may be
- Identifying patients who may benefit from FLT3-targeted drug therapies
- Supporting decisions about post-treatment care, including whether allogeneic stem cell transplantation (a procedure using donor stem cells) may be appropriate
How to Prepare and What to Expect
No special preparation is needed for this test. However, there are a few important steps to take before your appointment.
Do You Need to Fast?
No, fasting is not required for the FLT3 Mutation Detection Test. You may eat and drink as normal before sample collection.
Practical Tips Before Your Test
A little preparation helps ensure your sample is collected smoothly. Keep the following in mind:
- Bring a detailed clinical history, including your symptoms, previous test results, and any relevant medical records, as this is required for the test
- Inform your doctor of all current medications, supplements, and any previous cancer treatments you have received
- Discuss any history of blood disorders or prior bone marrow procedures with your doctor
- This test is typically ordered by a haematologist (a blood specialist) or an oncologist (a cancer specialist)
Step-by-Step Procedure
Two types of samples may be collected for this test: peripheral blood and bone marrow. The procedure for each is described below.
Peripheral Blood Collection:
- A healthcare professional cleans the skin over a vein, usually in the arm.
- A small needle is used to draw approximately 3 ml of blood into a lavender-top EDTA tube (a tube containing an anticoagulant to prevent clotting).
- The tube is inverted several times to mix the blood with the anticoagulant.
- A small bandage is applied to the collection site.
- The sample is labelled and stored at 2 to 8 degrees Celsius before dispatch to the laboratory.
Bone Marrow Aspirate Collection:
- A specialist performs a bone marrow aspiration, usually from the hip bone, under local anaesthesia (numbing medicine).
- A needle is inserted carefully to draw a small amount of liquid bone marrow into a sodium heparin (green-top) tube.
- The sample is labelled and kept refrigerated before being sent to the laboratory.
- Once received, the laboratory extracts DNA from the sample and performs a multiplex PCR (a technique to copy specific DNA segments), followed by capillary electrophoresis to separate and analyse the DNA fragments by size.
Factors That Can Affect Accuracy
The following factors may influence the reliability of your test results:
- Poor sample quality or incorrect storage during transport
- Low numbers of abnormal cells in the sample, which may lead to a false-negative result (the mutation is present but not detected)
- The test has a detection limit of approximately 10% mutation-bearing cells; samples with fewer abnormal cells may not yield a positive result
- Prior chemotherapy or bone marrow transplant can alter mutation patterns
- The FLT3 mutation status can change between initial diagnosis and relapse, so retesting may be needed
Understanding Your FLT3 (ITD, D835Y) Mutation Detection Test Results
Results are reported as positive or negative for each mutation type. A doctor will interpret these findings alongside the patient's clinical picture and other test results. The table below provides a general guide to what results may mean.
| Parameter | Normal Result | Interpretation if Detected |
|---|---|---|
| FLT3-ITD | Negative (Not Detected) | FLT3-ITD mutation detected; associated with increased relapse risk and more aggressive disease in AML |
| FLT3-D835Y (TKD) | Negative (Not Detected) | FLT3 tyrosine kinase domain mutation detected; clinical significance varies and depends on other genetic findings |
| ITD Allelic Ratio | Not Applicable if FLT3-ITD is Negative | If FLT3-ITD is present, a higher allelic ratio indicates a greater mutant burden and may be associated with a less favourable prognosis |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain circumstances can affect how results are interpreted.
Previous treatment with chemotherapy or bone marrow transplantation may alter the presence or absence of FLT3 mutations.
Studies have shown that FLT3 mutations can be gained or lost in cancer cells following treatment, which means results at relapse may differ from those at initial diagnosis. Retesting at relapse is therefore recommended.
How to Maintain Healthy Levels
Because the FLT3 mutation is a genetic change in cancer cells, lifestyle habits do not directly influence whether the mutation is present. General guidance for patients with AML includes the following:
- Follow your haematologist's recommendations for monitoring and follow-up appointments
- Maintain good overall health through adequate rest, balanced nutrition, and staying well-hydrated during treatment
- Keep all medical records organised, as your mutation status is an important part of your treatment history
Lupin Diagnostics FLT3 (ITD, D835Y) Mutation Detection Test Price
The FLT3 Mutation Detection Test cost at Lupin Diagnostics starts at ₹5,400. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 5400 |
| CHENNAI | 5400 |
| HYDERABAD | 5400 |
| KOLKATA | 5400 |
| NAVI MUMBAI | 5400 |
| PUNE | 5400 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Booking your FLT3 Mutation Detection Test online at Lupin Diagnostics is straightforward.
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection by a trained professional.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
FLT3 is a protein found on the surface of early blood cells that plays a role in normal blood cell development. When mutations occur in this gene, the protein becomes permanently active, causing blood cells to grow and divide in an uncontrolled way. This is closely linked to the development of AML.
FLT3 is the most frequently mutated gene in AML. Approximately 30% of newly diagnosed AML patients carry an FLT3 mutation, making this one of the most clinically important genetic markers in blood cancer.
The FLT3-ITD mutation involves a duplication of genetic material and is found in roughly 20% to 30% of FLT3-mutated AML cases. The FLT3-D835Y mutation is a point change in the tyrosine kinase domain and is seen in around 5% to 10% of cases. ITD mutations are generally associated with a more aggressive form of the disease.
A positive result helps your doctor understand the type and aggressiveness of your AML. FLT3-ITD positive patients are often considered for targeted drug therapies and may be evaluated for stem cell transplantation. Your haematologist will discuss what the result means specifically for your situation.
Both peripheral blood and bone marrow may be collected because bone marrow is where blood cell production occurs and may contain a higher concentration of abnormal cells. Blood samples are less invasive and are often collected alongside bone marrow aspirates. The choice of sample depends on clinical circumstances and your doctor's judgement.
Yes. FLT3 mutation status can change between initial diagnosis and relapse. Testing again at relapse is recommended because the results may differ and could affect which treatments are most suitable at that stage.
At Lupin Diagnostics, the report is delivered within 15 days of sample collection. In general, guidelines suggest that FLT3 mutation results should ideally be available within 2 to 5 calendar days, though turnaround times can vary between laboratories depending on the method used and sample volume.
FLT3 (ITD, D835Y) Mutation Detection Test: Booking, Price, and Results
