FISH - t(11;14) Test: Booking, Price, and Results
About FISH - t(11;14) Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | IGH-CCND1 Fusion FISH, CCND1/IGH t(11;14) FISH, t(11;14) Translocation FISH, Mantle Cell Lymphoma FISH, IGH-CCND1 Rearrangement FISH |
| Sample Type | Bone marrow aspirate or peripheral blood |
| Fasting Required | No fasting required |
| Report Time | 3 days |
| Recommended For | Adults with suspected or confirmed blood cancers such as mantle cell lymphoma, multiple myeloma, or chronic lymphocytic leukaemia |
| Price | Starting at ₹7,000 |
What is a FISH - t(11;14) Test?
The FISH - t(11;14) test is a specialised genetic test that detects an abnormal chromosomal rearrangement between chromosomes 11 and 14. This rearrangement, known as the t(11;14) translocation, causes a gene called CCND1 to fuse abnormally with the IGH gene, driving uncontrolled cell growth. The test is also called the IGH-CCND1 Fusion FISH or t(11;14) Translocation FISH test. It is typically ordered by a haematologist or oncologist when a blood cancer is suspected, using a bone marrow or blood sample.
What Does a FISH - t(11;14) Test Measure?
This test uses fluorescent probes to identify specific genetic changes at the chromosomal level. The key findings it looks for are listed below.
| Component | What It Means |
|---|---|
| t(11;14)(q13;q32) translocation | An abnormal joining of chromosomes 11 and 14 at specific locations |
| CCND1 (Cyclin D1) gene rearrangement | The CCND1 gene, which controls how cells divide, has moved to an abnormal position |
| IGH gene fusion | The immunoglobulin heavy chain gene has fused abnormally with CCND1, promoting unchecked cell division |
| Percentage of abnormal cells | The proportion of cells in the sample carrying this translocation |
When Cyclin D1 is overexpressed due to this translocation, cells lose normal control over division. This is a key event in the development of certain blood cancers, particularly mantle cell lymphoma.
Why is a FISH - t(11;14) Test Done?
A doctor may request this test to confirm a diagnosis, understand disease severity, or monitor a known blood cancer over time. Below are the most common reasons.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to request this or related haematological investigations.
- Swollen lymph nodes in the neck, groin, or armpits
- Unexplained fatigue or persistent weakness
- Drenching night sweats or recurrent unexplained fevers
- Unintended weight loss
- Bone pain, particularly in the back or limbs
- Shortness of breath or dizziness due to low red blood cell counts
- Frequent or unusual infections
- Hypercalcemia causing excessive thirst, nausea, or constipation.
- Impaired kidney function or kidney failure.
Conditions This Test Can Help Detect
This test helps identify or confirm several haematological (blood-related) conditions.
- Mantle cell lymphoma (MCL), where this translocation is found in 50 to 70% of cases
- Multiple myeloma, where t(11;14) is present in approximately 15 to 20% of newly diagnosed patients
- Chronic lymphocytic leukaemia (CLL)
- B-prolymphocytic leukaemia and plasma cell leukaemia
- Light chain amyloidosis, where this translocation occurs in 49 to 60% of patients
- Splenic lymphoma with villous lymphocytes
FISH - t(11;14) Test for Chronic Disease Monitoring
In patients already living with haematological malignancies, it plays an important role in ongoing care. It is recommended at initial diagnosis and again at disease progression to reassess genetic changes. Repeating the test at relapse helps doctors understand whether the disease has evolved and supports decisions about further management.
How to Prepare and What to Expect
No special preparation is needed before this test. The information below will help you understand what to expect on the day of collection.
Do You Need to Fast?
No. Fasting is not required for the FISH - t(11;14) test. You can eat and drink normally before your appointment.
Practical Tips Before Your Test
The following steps will help ensure your visit goes smoothly.
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Inform your doctor about all current medications, especially blood thinners or anticoagulants
- If this is a follow-up test, carry your earlier FISH or bone marrow reports
- Where possible, the test should be done before starting chemotherapy, as treatment can reduce the number of cancer cells available for analysis
- Follow any specific timing instructions given by your haematologist regarding sample collection
Step-by-Step Procedure
Two sample types may be collected for this test: bone marrow aspirate and peripheral blood. Both are described below.
Bone Marrow Sample Collection:
- You will be asked to lie down in a comfortable position, and the collection site (usually the hip bone) will be cleaned.
- A local anaesthetic is applied to numb the area, which may cause a brief stinging sensation.
- A small needle is used to withdraw approximately 3 ml of bone marrow fluid into a sodium heparin (green-top) tube.
- Light pressure is applied after the procedure to minimise discomfort.
Peripheral Blood Sample Collection:
- If a bone marrow sample is not feasible, a standard blood draw from a vein in your arm is performed.
- Approximately 3 ml of blood is collected into a lavender-top EDTA tube.
- The sample is labelled and kept refrigerated between 2 and 8 degrees Celsius for transport.
- In the laboratory, the sample is fixed and exposed to fluorescent probes that attach to specific chromosomal regions.
- A specialist examines 200 cell nuclei under a fluorescence microscope and records the percentage of abnormal cells detected.
Factors That Can Affect Accuracy
- Prior chemotherapy, which may reduce the number of cancer cells in the sample
- Insufficient cell count or low tumour involvement in the sample
- Poor sample transport conditions or delays in delivery
- Samples with very low plasma cell involvement (below 20%) may yield inconclusive results
- Laboratory probe quality and technical expertise
Understanding Your FISH - t(11;14) Test Results
Results from this test require careful interpretation by a qualified specialist, as the findings are meaningful only in the context of a patient's full clinical picture. The table below provides a general reference.
| Parameter | Normal (Negative) Result | Abnormal (Positive) Result |
|---|---|---|
| t(11;14) translocation | Not detected | Detected |
| Percentage of abnormal nuclei | Less than 5% (laboratory-specific cut-off) | 5% or above (laboratory-specific cut-off) |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
A negative result means no evidence of chromosomal rearrangement, deletion, or translocation was found.
A positive result indicates that the t(11;14) translocation is present and requires correlation with clinical and pathological findings by your doctor.
Results During Special Conditions
Certain circumstances can make results harder to interpret.
- Prior chemotherapy can destroy cancer cells that carry important diagnostic information, potentially affecting what the test can detect.
- When tumour burden is low or plasma cell involvement is insufficient, results may be inconclusive rather than clearly positive or negative.
- At low disease levels, it can also be difficult to distinguish between plasma cell conditions and mantle cell lymphoma based on this test alone.
How to Maintain Healthy Levels
For patients monitoring a known condition, the following general steps support ongoing care.
- Attend all scheduled follow-up appointments with your haematologist or oncologist without delay
- Report any new or worsening symptoms, such as fatigue, bone pain, or recurring infections, promptly to your care team
- Follow your prescribed treatment plan consistently and discuss any concerns with your doctor
Lupin Diagnostics FISH - t(11;14) Test Price
The FISH - t(11;14) test is priced starting at ₹7,000 at Lupin Diagnostics. This test requires a visit to a Lupin Diagnostics centre, as home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 7000 |
| CHENNAI | 7000 |
| HYDERABAD | 7000 |
| KOLKATA | 7000 |
| NAVI MUMBAI | 7000 |
| PUNE | 7000 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
This test detects a specific chromosomal rearrangement that is a key genetic marker in mantle cell lymphoma. It is also used to identify this translocation in other blood cancers, including multiple myeloma, chronic lymphocytic leukaemia, and light chain amyloidosis. Your doctor uses the result alongside other clinical and laboratory findings to confirm a diagnosis.
Two sample types may be used: a bone marrow aspirate collected in a sodium heparin tube, or a peripheral blood sample collected in an EDTA tube. Bone marrow is the preferred sample because it typically contains a higher number of cancer cells for analysis. Your haematologist will advise which sample is appropriate for your situation.
A peripheral blood draw involves a standard needle prick and is generally well-tolerated. Bone marrow aspiration is done under local anaesthesia and may cause brief pressure or discomfort during the procedure. Most patients manage this without difficulty.
The report is typically ready within 3 days of sample receipt at the laboratory. Your doctor will discuss the findings with you once the report is available.
A positive result means the t(11;14) translocation was detected in the sample. This finding is strongly associated with mantle cell lymphoma and may also be seen in multiple myeloma and other conditions. A positive result alone does not confirm a diagnosis; your specialist will review it alongside other tests and your clinical history.
You can book this test online through the Lupin Diagnostics website. However, home collection is not available for this test. Sample collection requires a visit to a Lupin Diagnostics centre where trained staff can handle the specialised sample types involved.
Yes, it can. Chemotherapy given before the test may reduce the number of detectable cancer cells in the sample, which could affect the sensitivity of the result. Where possible, your haematologist may recommend performing the test before treatment begins or at a time when the disease is measurable.
FISH - t(11;14) Test: Booking, Price, and Results
