FISH - IgH/MYC t(8;14) Test: Booking, Price, and Results
About FISH - IgH/MYC t(8;14) Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | IGH/MYC Translocation FISH Test, MYC/IgH t(8;14) FISH Test, Burkitt Lymphoma FISH Test, c-MYC/IgH Dual Fusion FISH Test |
| Sample Type | Peripheral blood and bone marrow sample |
| Fasting Required | No fasting required |
| Report Time | 3 days |
| Recommended For | Adults and children (male and female) with suspected B-cell lymphoma or leukaemia |
| Price | Starting at ₹4,600 |
What Is a FISH - IgH/MYC t(8;14) Test?
The FISH - IgH/MYC t(8;14) test is a specialised genetic test that detects a specific chromosomal abnormality linked to certain blood cancers. It uses fluorescent probes to identify whether a translocation (a segment of one chromosome breaking off and attaching to another) has occurred between chromosomes 8 and 14. This rearrangement involves the MYC gene and the IGH gene. Doctors typically order this test when a patient shows signs of an aggressive B-cell blood cancer such as Burkitt lymphoma or acute lymphoblastic leukaemia. This test is also known as the IGH/MYC translocation FISH test, MYC/IgH t(8;14) FISH test, Burkitt lymphoma FISH test, or c-MYC/IgH dual fusion FISH test. It requires peripheral blood drawn from a vein and a bone marrow sample collected at a hospital.
What Does a FISH - IgH/MYC t(8;14) Test Measure?
This FISH - IgH/MYC t test analyses the genetic material in blood or bone marrow cells to look for a specific chromosomal change. Here is what the test examines:
| Parameter | What It Detects |
|---|---|
| IgH/MYC t(8;14) Translocation | Whether the MYC gene on chromosome 8 has fused with the IGH gene on chromosome 14, producing an abnormal gene arrangement linked to B-cell malignancies |
A normal (negative) result shows two separate green and two separate orange fluorescent signals, meaning no fusion has occurred. A positive result shows fusion signals where the two gene regions have joined together.
Why Is a FISH - IgH/MYC t(8;14) Test Done?
Doctors order the FISH - IgH/MYC t(8;14) test to investigate symptoms that may point to an underlying blood cancer or to confirm a suspected diagnosis. It plays an important role in both initial diagnosis and ongoing monitoring.
Common Symptoms That May Require This Test
Several symptoms may prompt a doctor to request this test. These include:
- Swollen lymph nodes in the neck, armpit, or groin
- Persistent fatigue and unexplained weight loss
- Drenching night sweats and fever
- Abdominal pain, swelling, or bloating
- Nausea, vomiting, or gastrointestinal bleeding
- Jaw swelling or swelling around the eyes
Conditions This Test Can Help Detect
The FISH - IgH/MYC t(8;14) test can help identify several blood and lymph node conditions:
- Burkitt lymphoma, an aggressive high-grade B-cell non-Hodgkin's lymphoma strongly associated with this translocation
- Diffuse large B-cell lymphoma (DLBCL), a common aggressive non-Hodgkin lymphoma
- Acute lymphoblastic leukaemia (ALL), certain cases of this blood cancer
- Multiple myeloma, a plasma cell malignancy
- Chronic lymphocytic leukaemia (CLL), a slow-growing blood cancer
FISH - IgH/MYC t(8;14) Test for Chronic Disease Monitoring
Once a B-cell malignancy is confirmed, this test may be repeated to assess how well treatment is working. It can detect residual disease after therapy and identify signs of relapse. Oncologists use these results alongside clinical findings to guide ongoing care decisions.
How to Prepare and What to Expect
Preparation differs depending on which sample is being collected. Here are a few practical steps that will help the process go smoothly.
Do You Need to Fast?
No, fasting is not required for the FISH - IgH/MYC t(8;14) test. You can eat and drink normally before your sample collection. However, if your doctor plans to use sedation during the bone marrow collection, you may need to follow specific instructions before the procedure. Confirm this with your doctor in advance.
Practical Tips Before Your Test
A few simple steps will help ensure accurate results:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Inform your doctor about all medications you are taking, especially blood thinners
- Stay well hydrated before the blood draw
- Wear loose, comfortable clothing that allows easy access to your arm
- If a bone marrow sample is required, follow your treating doctor's specific pre-procedure instructions closely
Step-by-Step Procedure
- For a peripheral blood sample, a trained phlebotomist draws approximately 3 ml of blood from a vein in your arm using a green-top tube containing sodium heparin.
- The tube is gently inverted to mix the blood with the anticoagulant. The sample is not frozen or centrifuged and is kept cool during transport to the laboratory.
- If a bone marrow sample is needed, this is performed at a hospital by a trained medical professional. The back of the hip bone (posterior superior iliac crest) is the most commonly used collection site.
- The area is cleaned, and a local anaesthetic is applied to minimise discomfort. A small needle is then used to draw approximately 3 ml of bone marrow aspirate into a sodium heparin tube.
- Both samples are sent to the NABL-accredited laboratory, where fluorescent probes are applied to chromosomes to identify the IgH/MYC translocation under a fluorescence microscope.
Factors That Can Affect Accuracy
Certain factors may influence the reliability of your result:
- A low number of abnormal cells in the sample (low tumour burden) can make detection harder
- Poor sample quality or use of the wrong anticoagulant may affect results
- Delayed or incorrect transport conditions can reduce sample viability
- Tissue that has been decalcified or improperly fixed may cause the test to fail
Understanding Your FISH - IgH/MYC t(8;14) Test Results
A doctor, usually a haematologist or oncologist, must review the result alongside your symptoms, clinical history, and other investigations. The table below shows the interpretation of test results:
| Result | Interpretation |
|---|---|
| Negative (Normal) | No IgH/MYC gene fusion detected; the t(8;14) translocation is absent |
| Positive (Abnormal) | The t(8;14) translocation is present, indicating a gene fusion linked to B-cell malignancies such as Burkitt lymphoma, DLBCL, or ALL |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain situations can influence how results are interpreted:
- A low number of cancer cells in the sample may produce a false negative, meaning the translocation is present but not detected. Repeat testing or a MYC break-apart FISH test may be recommended.
- Poor sample quality due to improper fixation or delayed transport can compromise the analysis and may require a repeat sample.
- Prior cancer treatment may reduce the proportion of abnormal cells, potentially affecting detection.
How to Maintain Healthy Levels
The following tips focus on supporting overall health:
- Support your immune system with balanced nutrition, adequate sleep, and regular physical activity
- Attend all follow-up appointments as advised by your oncologist or haematologist
- Report any new or worsening symptoms, such as persistent swelling, unexplained weight loss, or prolonged fever, to your doctor without delay
Lupin Diagnostics FISH - IgH/MYC t(8;14) Test Price and Home Collection
The FISH - IgH/MYC t(8;14) test cost at Lupin Diagnostics starts at ₹4,600. Home collection is available for peripheral blood samples. For bone marrow sample collection, you will need to visit a hospital as directed by your doctor. The table below shows indicative prices:
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 4600 |
| CHENNAI | 4600 |
| HYDERABAD | 4600 |
| KOLKATA | 4600 |
| NAVI MUMBAI | 4600 |
| PUNE | 4600 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
Home Collection
FISH - IgH/MYC t(8;14) test home collection is available for peripheral blood samples across cities. A certified phlebotomist visits your home to collect the blood sample at your chosen time. All samples are processed in NABL-accredited laboratories, and your digital report is delivered directly to you via email or WhatsApp.
For bone marrow sample collection, visit a hospital as directed by your doctor.
How to Book
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist for the peripheral blood sample, or visit your nearest Lupin Diagnostics centre. For bone marrow aspiration, visit a hospital as directed by your doctor.
- Receive your report via email or WhatsApp within 3 days.
Frequently Asked Questions
This test detects a specific chromosomal translocation between the MYC gene on chromosome 8 and the IgH gene on chromosome 14. It is primarily used to diagnose Burkitt lymphoma and certain cases of acute lymphoblastic leukaemia and to help guide treatment decisions in patients with suspected B-cell malignancies.
A positive result means the IgH/MYC translocation has been detected in your sample. This finding is seen in around 85% of Burkitt lymphoma cases and in a proportion of diffuse large B-cell lymphoma and acute lymphoblastic leukaemia cases. Your oncologist will review the result alongside your full clinical picture before making any diagnosis or treatment decision.
FISH - IgH/MYC t(8;14) test home collection is available for peripheral blood samples. If your doctor has requested a bone marrow sample, this must be collected at a hospital by a trained specialist, as it is a clinical procedure performed under local anaesthesia.
At Lupin Diagnostics, the results are typically delivered within 3 days of the laboratory receiving the sample. Your digital report is delivered directly to you via email or WhatsApp.
Yes, in some circumstances. If the MYC gene rearrangement involves a partner chromosome other than IgH (such as chromosomes 2 or 22), this specific test will not detect it. Your doctor may request additional genetic tests if clinical concern remains after a negative result.
Doctors may request both a peripheral blood sample and a bone marrow aspirate depending on the stage of investigation or the type of cancer suspected. Bone marrow samples can reveal abnormalities that may not be detectable in circulating blood alone, making them particularly useful when lymphoma or leukaemia is strongly suspected.
FISH - IgH/MYC t(8;14) Test: Booking, Price, and Results
