FGFR1 (Solid Tumour) Test: Booking, Price, and Results
About FGFR1 (Solid Tumour) Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | FGFR1 Amplification Test, FGFR1 FISH, Fibroblast Growth Factor Receptor 1 Test, FGFR1 Gene Amplification by FISH |
| Sample Type | Formalin-fixed paraffin-embedded (FFPE) tumour tissue block |
| Fasting Required | No fasting required |
| Report Time | 8 days |
| Recommended For | Adults with solid tumours, particularly squamous cell carcinomas of the lung, oesophagus, and head and neck, and breast cancer patients |
| Price | Starting at ₹8,400 |
What is an FGFR1 (Solid Tumour) Test?
The FGFR1 test checks tumour tissue for amplification of the FGFR1 gene, which controls cell growth and division. When this gene is overactive, it can drive tumour progression in certain cancers. Oncologists order this test to guide treatment decisions for patients diagnosed with solid tumours. Also known as FGFR1 FISH or the Fibroblast Growth Factor Receptor 1 Test, it uses a specialised technique called fluorescence in situ hybridisation to examine the tumour at a genetic level.
What Does a FGFR1 (Solid Tumour) Test Measure?
This test analyses specific genetic features within a tumour tissue sample. The table below explains what each measurement looks at and why it matters.
| Parameter | What It Means |
|---|---|
| FGFR1 gene copy number | Counts how many copies of the FGFR1 gene are present in each tumour cell |
| D8Z2 (chromosome 8 centromere) copy number | Counts the reference copies on chromosome 8 to provide a baseline for comparison |
| FGFR1:D8Z2 ratio | Compares FGFR1 copies to chromosome 8 baseline; a ratio above 2.0 or 6 or more copies per nucleus indicates amplification |
Why is an FGFR1 (Solid Tumour) Test Done?
Doctors request this test when a patient's tumour needs to be evaluated at a molecular level to support treatment planning. It provides information about gene amplification that standard imaging or routine pathology cannot reveal.
Common Symptoms That May Require This Test
The symptoms below are not caused by FGFR1 amplification directly. Rather, they are signs that lead a doctor to investigate an underlying cancer, after which this test may be ordered.
- Unexplained and persistent weight loss
- Pain, palpable lumps, or swelling at the tumour site
- Chronic cough that does not resolve
- Chest pain or tightness
- Difficulty breathing
- A lump or mass detected on imaging
- Advanced cancer staging confirmed at diagnosis
Conditions This Test Can Help Detect
This test is used to assess FGFR1 amplification status across several cancer types. The following conditions are commonly evaluated:
- Squamous cell lung cancer (FGFR1 amplification found in approximately 20% of cases)
- Breast cancer (amplification present in roughly 10% of cases)
- Head and neck squamous cell carcinoma (10 to 17% of cases)
- Oesophageal squamous cell carcinoma (6 to 21% of cases)
- Small cell lung cancer (approximately 6% of cases)
- Prostate cancer and oral squamous cell carcinoma
How to Prepare and What to Expect
Because this test uses tumour tissue rather than a blood or urine sample, preparation is different from a routine diagnostic test. The information below covers what you and your care team need to keep in mind.
Do You Need to Fast?
No fasting is required for this test. The sample is tissue from a biopsy or surgical procedure, not a blood draw, so food and drink do not affect the sample.
Practical Tips Before Your Test
Here are a few practical steps to ensure the test runs smoothly:
- Bring a detailed clinical history including your symptoms, previous test results, and relevant medical records, as this is required for the test
- Confirm with your surgeon or pathologist that the tissue will be fixed in 10% neutral buffered formalin before embedding
- Inform your oncologist about any prior cancer treatments, surgeries, or transplant history
- Note that fixatives other than formalin (such as Bouin's solution) may not work for this type of analysis, so communicate this to your pathologist in advance
Step-by-Step Procedure
The sample for this test is collected during a surgical or interventional procedure. Here is what the process typically involves:
- A surgeon or interventional specialist obtains tumour tissue through a biopsy or surgical resection.
- The tissue is fixed in formalin and embedded in paraffin wax to create a preserved tissue block (FFPE block).
- The FFPE block is transported to the laboratory at ambient temperature (18 to 28 degrees Celsius).
- Laboratory technicians apply fluorescent probes that attach to the FGFR1 gene region and the chromosome 8 centromere.
- A trained pathologist examines at least 20 non-overlapping tumour cells under a fluorescence microscope and counts gene and centromere copies.
- Results are calculated and reported, with the FGFR1:D8Z2 ratio used to determine amplification status.
Factors That Can Affect Accuracy
- Poor tissue fixation quality (incorrect formalin type or fixation time)
- Insufficient tumour cells in the sample
- Prior decalcification of the tissue block, which reduces the success rate to approximately 50%
- Damage during sample transport
- Slides submitted without an accompanying pathology report, which may delay or compromise interpretation
Understanding Your FGFR1 (Solid Tumour) Test Results
Results are reported as either positive (amplification detected) or negative (no amplification detected). Your oncologist will review these findings alongside your overall clinical picture and pathology report. The table below summarises how results are interpreted.
| Result | FGFR1:D8Z2 Ratio | Copies Per Nucleus | Interpretation |
|---|---|---|---|
| Negative | 2.0 or below | Fewer than 6 | No FGFR1 amplification detected |
| Positive | Above 2.0 OR | 6 or more | FGFR1 amplification detected |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
A negative result does not exclude the presence of a tumour or cancer. This test specifically looks for FGFR1 amplification, not cancer as a whole.
A positive result indicates gene amplification and may assist your oncologist in making treatment decisions. Patients with FGFR1 amplification may be candidates for targeted therapies that specifically act on this pathway.
How to Maintain Healthy Levels
FGFR1 amplification is a genetic change in tumour tissue and is not influenced by diet or exercise. That said, the following general points are worth keeping in mind:
- Smoking has been linked to a higher prevalence of FGFR1 amplification in lung cancer patients; avoiding tobacco is a meaningful step
- Follow your oncologist's guidance for cancer monitoring, follow-up testing, and treatment
- Attend all scheduled clinical reviews so any changes in your condition are caught early
Lupin Diagnostics FGFR1 (Solid Tumour) Test Price
The FGFR1 test cost at Lupin Diagnostics starts at ₹8,400. This test requires tumour tissue collected during a biopsy or surgical procedure and must be performed at a Lupin Diagnostics centre. Home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 8400 |
| CHENNAI | 8400 |
| HYDERABAD | 8400 |
| KOLKATA | 8400 |
| NAVI MUMBAI | 8400 |
| PUNE | 8400 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample submission.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The FGFR1 test is used to detect amplification of the FGFR1 gene in solid tumour tissue. The result helps oncologists assess prognosis and decide whether a patient may benefit from targeted treatment aimed at the FGFR1 pathway.
This test is most commonly used for squamous cell carcinomas of the lung, oesophagus, and head and neck region, as well as breast cancer. It may also be used for thymic tumours and other solid tumour types where FGFR1 amplification has clinical relevance.
The sample is tumour tissue obtained through a surgical biopsy or resection. The tissue is fixed in formalin and embedded in paraffin to create an FFPE block, which is then sent to the laboratory for FGFR1 FISH analysis. There is no blood draw or home collection involved.
A positive result means the FGFR1 gene is amplified in the tumour cells. This information can help your oncologist decide whether therapies targeting the FGFR1 protein are appropriate for your treatment plan. Your doctor will explain what this means for your specific case.
No. A negative result means FGFR1 amplification was not detected in the sample. It does not rule out cancer or other genetic alterations. Your oncologist will interpret this result alongside all other clinical and pathological findings.
At Lupin Diagnostics, the report is delivered within 8 days of the laboratory receiving the sample. Turnaround time may vary if the sample requires additional processing or if the tissue quality affects the analysis.
Yes. A detailed clinical history, including your diagnosis, previous test results, and pathology report, is required before the laboratory can process and interpret the sample. Submitting this information at the time of booking helps avoid any delays in reporting.
FGFR1 (Solid Tumour) Test: Booking, Price, and Results
