Factor V Mutation Screening (F5 - G1691A) Test
About Factor V Mutation Screening (F5 - G1691A) Test
| Field | Value |
|---|---|
| Also Known As | Factor V Leiden Mutation Test, FVL Test, F5 G1691A Mutation Test, APC Resistance Test, Factor V R506Q Mutation Test |
| Sample Type | Peripheral blood (EDTA tube) |
| Fasting Required | No fasting required |
| Report Time | 5 days |
| Recommended For | All genders and ages; particularly those with a personal or family history of blood clots |
| Price | Starting at ₹6,000 |
What is a Factor V Mutation Screening (F5 - G1691A) Test?
The factor V mutation screening (F5 - G1691A) test is a genetic test that checks for a specific change in the F5 gene, known as the Factor V Leiden mutation. This mutation can cause the blood to clot more easily than normal, raising the risk of abnormal clots in the veins. A doctor may order this test when someone has unexplained blood clots, recurrent miscarriages, or a family history of clotting disorders. The test requires a small sample of peripheral blood collected in an EDTA tube. The test is also known as the factor V Leiden mutation test, FVL test, F5 G1691A mutation test, APC resistance test, or factor V R506Q mutation test.
What Does a Factor V Mutation Screening (F5 - G1691A) Test Measure?
This test analyses your DNA to look for a specific genetic change and its effect on how your blood controls clotting. Here is what it checks for:
| Parameter | What It Shows |
|---|---|
| F5 G1691A Mutation Status | Whether the mutation is present or absent in your DNA |
Why Is a Factor V Mutation Screening (F5 - G1691A) Test Done?
Doctors order the factor V mutation screening (F5 - G1691A) test to investigate unexplained clotting events and to assess clotting risk before certain medical procedures.
Common Symptoms That May Require This Test
Certain symptoms may prompt a doctor to request this test. These include:
- Leg pain, swelling, redness, or warmth, which may indicate a blood clot in a deep vein
- Shortness of breath or chest pain, which may suggest a clot in the lungs
- Unexplained coughing or difficulty breathing
- Swelling or pain in the arm
- Recurrent miscarriages with no clear cause
Conditions This Test Can Help Detect
This test can help identify or assess the risk of several clotting-related conditions.
- Deep vein thrombosis (DVT), blood clots forming in deep veins, most often in the legs
- Pulmonary embolism (PE), a blood clot that has travelled to the lungs
- Thrombophilia, a general tendency for the blood to clot abnormally
- Recurrent pregnancy loss linked to a clotting disorder
- Elevated clotting risk before surgery, hormone therapy, or use of oral contraceptives
How to Prepare and What to Expect
The F5 - G1691A test procedure is straightforward and requires minimal preparation. Here is what you need to know before your appointment.
Do You Need to Fast?
No fasting is required before this test. You can eat and drink normally on the day of your sample collection. If additional tests are scheduled along with this test, check with your doctor if they need fasting.
Practical Tips Before Your Test
A few simple steps will help the process go smoothly:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history of clotting disorders, as this is required for the test
- Tell your doctor about all medications you are taking, especially blood-thinning medicines such as warfarin
- Stay well hydrated before your appointment
- Wear a loose-fitting top or short sleeves for easy access to your arm during blood collection
Step-by-Step Procedure
Here is what to expect during the F5 - G1691A test procedure:
- A trained phlebotomist cleans a small area on your arm and locates a suitable vein.
- A fine needle is inserted to draw approximately 2 ml of blood into a lavender-top EDTA tube. You may feel a brief, mild pinch.
- The needle is removed, and light pressure is applied to the collection site.
- The blood sample is labelled and stored at 2 to 8°C for safe transport.
- The sample is sent to the NABL-accredited laboratory for analysis using real-time PCR (polymerase chain reaction) technology.
- The laboratory analyses your DNA to detect the presence or absence of the F5 G1691A mutation and reports the result within 5 days.
Factors That Can Affect Accuracy
Certain factors may influence the reliability of your test result. These include:
- A recent bone marrow transplant from a donor, which can interfere with DNA-based testing
- Blood transfusions received close to the time of testing
- Very high levels of heparin or the presence of lupus anticoagulant, which may affect the APC resistance ratio
- Liver disease or certain medications, including hormonal contraceptives
- Mislabelled samples or inaccurate clinical information provided at the time of testing
Understanding Your Factor V Mutation Screening (F5 - G1691A) Test Results
Your results will indicate whether the mutation was detected and, if so, whether you carry one or two copies. A qualified doctor should always review these findings in the context of your full medical history. The table below shows typical result categories:
| Result | Interpretation |
|---|---|
| Not Detected (Wild Type) | No mutation found; normal Factor V function expected |
| Heterozygous (one copy detected) | One copy of the mutation present; moderately increased clotting risk |
| Homozygous (two copies detected) | Two copies of the mutation present; significantly increased clotting risk |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain situations can affect how results are interpreted:
- Patients who have had a bone marrow transplant from an allogeneic (unrelated or related) donor may receive unreliable DNA-based results, as the blood cells tested will carry the donor's genetic profile.
- An indeterminate APC resistance ratio may occur in patients with very high heparin levels or those with a lupus anticoagulant; the DNA mutation test itself remains unaffected by these conditions.
- Pregnancy can alter some coagulation-based screening results, though the DNA mutation component of this test is not affected by pregnancy itself.
How to Maintain Healthy Levels
Since this is a genetic test, the result itself does not change. However, lifestyle choices can help reduce clotting risk if the mutation is present:
- Avoid smoking and limit alcohol, as both can raise the risk of blood clots.
- During long journeys by air or road, move your legs regularly; rotate your ankles and flex your feet every hour to keep blood circulating.
- Stay well hydrated and maintain a healthy weight to support good circulation.
Lupin Diagnostics Factor V Mutation Screening (F5 - G1691A) Test Price and Home Collection
The F5 - G1691A test cost at Lupin Diagnostics starts at ₹6,000, and home sample collection is available across multiple cities in India. The table below provides indicative prices:
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 6000 |
| CHENNAI | 6000 |
| HYDERABAD | 6000 |
| KOLKATA | 6000 |
| NAVI MUMBAI | 6000 |
| PUNE | 6000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps for F5 - G1691A test online booking:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Home Collection
The F5 - G1691A test home collection is available across cities, making it convenient to get tested without visiting a centre. All samples are processed in NABL-accredited laboratories by experienced professionals. Your digital report is delivered securely via email or WhatsApp once ready.
Frequently Asked Questions
Factor V Leiden is an inherited genetic mutation that affects the way your blood clots. People who carry this mutation have a higher risk of developing abnormal blood clots in their veins. The factor V mutation screening (F5 - G1691A) test identifies whether you carry this mutation and, if so, how many copies you have inherited.
This test is generally recommended for people who have had an unexplained blood clot, especially before the age of 50 or when a clot has formed in an unusual location such as the liver, kidneys, or brain. It is also considered for those with a close family member who has been diagnosed with factor V Leiden or a clotting disorder.
No, fasting is not required. You can eat and drink as usual before giving your blood sample. Follow any additional instructions your doctor may provide.
A heterozygous result means you have inherited one copy of the mutated gene and one normal copy. A homozygous result means both copies of the gene carry the mutation. The homozygous form is associated with a considerably higher clotting risk than the heterozygous form.
Not necessarily. Carrying the Factor V Leiden mutation increases your clotting risk, but it does not guarantee that you will develop a blood clot. Only about 10% of individuals with this mutation ever experience an abnormal clotting event. Your doctor will assess your overall risk based on other health and lifestyle factors.
This is a one-time genetic test. Because your DNA does not change over time, the result remains the same throughout your life. There is no need to repeat it for monitoring purposes unless there is a specific clinical reason, such as a suspected sample labelling error.
At Lupin Diagnsotics, results are typically available within 5 days of sample collection. You will receive your report digitally via email or WhatsApp once it is ready.
Factor V Mutation Screening (F5 - G1691A) Test
