Factor II Mutation Screening (F2 - G20210A) Test
About Factor II Mutation Screening (F2 - G20210A) Test
| Field | Value |
|---|---|
| Also Known As | Prothrombin G20210A Mutation Test, Factor II Mutation Test, Prothrombin Gene Mutation Test, FII G20210A Mutation Test |
| Sample Type | Peripheral blood |
| Fasting Required | No fasting required |
| Report Time | 5 days |
| Recommended For | Adults of all genders; particularly those with a personal or family history of blood clots |
| Price | Starting at ₹6,000 |
What Is a Factor II Mutation Screening (F2 - G20210A) Test?
The factor II mutation screening (F2 - G20210A) test is a genetic test that checks for a specific inherited mutation in the F2 gene (the prothrombin gene). This mutation can cause the body to produce excess prothrombin, a protein involved in blood clotting, raising the risk of abnormal clots. It is typically ordered when a doctor suspects an inherited clotting disorder. This test is also called the prothrombin G20210A mutation test, factor II mutation test, prothrombin gene mutation test, or FII G20210A mutation test. A small blood sample is collected from a vein in the arm.
What Does a Factor II Mutation Screening (F2 - G20210A) Test Measure?
This test analyses DNA from your blood to identify whether a specific genetic change is present in the prothrombin gene. Here is what it looks for:
| Parameter | What It Tells You |
|---|---|
| F2 G20210A Mutation Status | Whether the genetic change at position 20210 of the F2 gene is present or absent |
Why Is a Factor II Mutation Screening (F2 - G20210A) Test Done?
A doctor may order this test to investigate unexplained blood clots or to assess inherited clotting risk. The following sections outline the common reasons.
Common Symptoms That May Require This Test
The following symptoms may prompt your doctor to recommend this test:
- Pain, swelling, or redness in the leg or arm, possibly with warmth to the touch
- Sudden, unexplained shortness of breath
- Chest pain that worsens when taking a deep breath
- Coughing, sometimes with blood
- History of unexplained blood clots at a young age
Conditions This Test Can Help Detect
This test can help identify or assess the following conditions:
- Prothrombin thrombophilia, an inherited clotting disorder that increases the risk of deep-vein thrombosis (DVT) and pulmonary embolism (a clot in the lungs)
- Hyperprothrombinemia, where excess prothrombin protein raises the risk of venous thromboembolism (VTE)
- Blood clots in unusual locations, such as cerebral venous sinuses (veins in the brain)
- Inherited clotting disorder risk before surgery, long-haul travel, or starting hormonal therapy
How to Prepare and What to Expect
No special preparation is needed for the factor II mutation screening (F2 - G20210A) test. The process is straightforward and involves a routine blood draw.
Do You Need to Fast?
No fasting is required. You can eat and drink normally before the test. If additional blood tests have been ordered alongside this one, check with your doctor whether those require fasting.
Practical Tips Before Your Test
Keep the following in mind before you go for your test:
- Bring a detailed clinical history, including your symptoms, any previous clotting events, current medications, and family history of clotting disorders, as this information is required for the test
- Tell your doctor about all medications you take, especially blood thinners or anticoagulants
- Share any personal or family history of DVT, pulmonary embolism, or known clotting disorders
- Wear a short-sleeved top or loose clothing so the phlebotomist can access your arm easily
- Inform your doctor if you have had a bone marrow or stem cell transplant from a donor, as this can interfere with the test result
Step-by-Step Procedure
The F2 - G20210A test procedure involves a straightforward blood draw. Here is what to expect:
- A trained phlebotomist will clean the skin on your inner arm with an antiseptic solution.
- A small amount of blood (2 ml) will be drawn from a vein, usually in the elbow crease.
- The blood is collected into an EDTA tube (a lavender-capped tube containing anticoagulant to keep the sample stable).
- Gentle pressure is applied to the puncture site with a cotton ball or small bandage to stop any bleeding.
- The sample is stored at the correct refrigerated temperature and sent to the molecular genetics laboratory.
- The laboratory analyses the DNA using real-time PCR (a technique that amplifies and identifies specific DNA sequences) to detect the G20210A mutation.
Factors That Can Affect Accuracy
Certain factors may affect the reliability of results. These include:
- A recent blood transfusion, which may introduce donor DNA
- A previous allogenic stem cell or bone marrow transplant from a donor (especially if the donor's genetic profile differs from yours)
- Mislabelled or improperly handled samples
- Somatic mosaicism (rare cases where only some cells carry the mutation)
- Genetic variants that are similar to but distinct from the target mutation
Understanding Your Factor II Mutation Screening (F2 - G20210A) Test Results
Your result will be reported as negative, heterozygous, or homozygous. Your doctor will explain what your result means for your individual health situation. The table below summarises the possible outcomes:
| Result | Genotype | Interpretation |
|---|---|---|
| Negative | Wild Type (GG) | No mutation detected; this specific clotting risk is not present |
| Positive (Heterozygous) | GA | One copy of the mutation; two to fivefold increased relative risk of DVT |
| Positive (Homozygous) | AA | Two copies of the mutation; significantly increased clot risk; rare |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain medical circumstances can affect how results are interpreted:
- Patients who have received an allogenic stem cell transplant before blood collection may show false-normal or unexpected results, depending on the donor's genotype.
- A recent blood transfusion can introduce donor DNA and may affect test accuracy. Inform your doctor before the test if either applies to you.
How to Maintain Healthy Levels
Since this is a genetic test, the result itself does not change. However, if you carry the mutation, the following general lifestyle measures may help reduce overall clot risk:
- Stay active and avoid sitting still for long periods, especially during long flights or road journeys
- Maintain a healthy body weight, as obesity is an additional risk factor for blood clots
- Discuss the use of oestrogen-containing contraceptives with your doctor, as these can significantly raise clotting risk in those who carry the mutation
Lupin Diagnostics Factor II Mutation Screening (F2 - G20210A) Test Price and Home Collection
The F2 - G20210A test cost at Lupin Diagnostics starts at ₹6,000, and home sample collection is available across cities. The table below shows city-wise pricing:
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 6000 |
| CHENNAI | 6000 |
| HYDERABAD | 6000 |
| KOLKATA | 6000 |
| NAVI MUMBAI | 6000 |
| PUNE | 6000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
The F2 - G20210A test online booking process at Lupin Diagnostics is straightforward:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Home Collection
Lupin Diagnostics offers the F2 - G20210A test home collection across multiple cities in India. Your blood sample is collected by a certified phlebotomist at your preferred time and location. All samples are processed in NABL-accredited laboratories, and your digital report is shared securely via email or WhatsApp.
Frequently Asked Questions
The F2 G20210A mutation is an inherited change in the prothrombin gene that causes the body to produce more prothrombin (a blood-clotting protein) than it needs. This excess prothrombin raises the likelihood of developing abnormal blood clots. People with this mutation are said to have an inherited thrombophilia, which is a tendency for the blood to clot more easily than normal.
This test is generally recommended for people who have had a DVT, a pulmonary embolism, or a blood clot in an unusual location. It may also be advised if a close family member has been diagnosed with a clotting disorder or if you have experienced a heart attack or stroke at a young age. Your doctor will advise whether the test is appropriate for you.
No fasting is required for this test. You can eat, drink, and take your regular medications as usual before the blood draw. If any other tests have been ordered at the same time, confirm their requirements separately with your doctor.
Not necessarily. Many people who carry this mutation, whether heterozygous or homozygous, never develop a blood clot in their lifetime. The mutation increases risk but does not guarantee that a clot will form. Your doctor will assess your overall risk profile, taking other factors into account.
Yes, this is an important consideration. Women who carry the mutation and use oestrogen-containing oral contraceptives face a significantly higher risk of DVT. Speak with your doctor before starting or continuing any hormonal contraceptive to discuss safer alternatives.
Yes. The F2 G20210A mutation is inherited, which means a carrier can pass one copy of the mutation to their children. If your result is positive, your doctor may recommend genetic counselling to help you understand the implications for family planning and the potential risk to close relatives.
Factor II Mutation Screening (F2 - G20210A) Test
