Deletion 8p FGFR-1 by FISH Test: Booking, Price, and Results
About Deletion 8p FGFR-1 by FISH Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | FGFR1 FISH, FGFR1 Break Apart FISH, 8p11 Rearrangement FISH, FGFR1 Gene Rearrangement Test |
| Sample Type | Bone marrow aspirate and peripheral blood (venous blood) |
| Fasting Required | No, unless sedation is used during bone marrow collection |
| Report Time | 8 days |
| Recommended For | All ages and genders; patients with suspected myeloproliferative neoplasms, eosinophilia, or lymphoma |
| Price | Starting at ₹4,700 |
What is a Deletion 8p FGFR-1 by FISH Test?
The Deletion 8p FGFR-1 by FISH test is a specialised laboratory test that detects rearrangements or deletions in the FGFR1 gene, located on chromosome 8p11. It uses a technology called fluorescence in situ hybridisation (FISH), which employs fluorescent probes to identify changes within specific gene regions. The test is ordered when a doctor suspects rare blood cancers linked to FGFR1 gene abnormalities. It is also known as the FGFR1 FISH or 8p11 Rearrangement FISH test.
What Does a Deletion 8p FGFR-1 by FISH Test Measure?
The Deletion 8p FGFR-1 by FISH test procedure analyses the structure of the FGFR1 gene to check whether it has been disrupted. Here is what the test examines:
| Component | What It Detects |
|---|---|
| FGFR1 gene rearrangement | Whether the FGFR1 gene has broken apart or fused with another gene on a different chromosome |
| Signal pattern analysis | Whether fluorescent probe signals appear fused (normal) or split (abnormal), indicating a chromosomal break |
| Chromosomal translocation | Whether FGFR1 has joined with a tyrosine kinase-coding gene, which can trigger uncontrolled cell growth |
Why is a Deletion 8p FGFR-1 by FISH Test Done?
Doctors order the Deletion 8p FGFR-1 by FISH when certain symptoms or blood test results suggest a rare blood cancer or genetic abnormality.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to recommend this test:
- Persistent fatigue without a clear cause
- Unexplained weight loss
- Drenching night sweats
- Recurrent or prolonged fever
- Swollen lymph nodes (lymphadenopathy)
- Enlarged spleen or liver (splenomegaly or hepatomegaly)
- Abnormally high white blood cell or eosinophil counts on routine blood tests
Conditions This Test Can Help Detect
The FGFR1 gene rearrangement test can help identify the following conditions:
- 8p11 myeloproliferative syndrome (also called myeloid and lymphoid neoplasm with FGFR1 abnormalities)
- Acute myeloid leukaemia (AML)
- T-lymphoblastic lymphoma with eosinophilia
- Chronic eosinophilic leukaemia
- B-lymphoblastic transformation of chronic eosinophilic leukaemia
- Mixed phenotype acute leukaemia (MPAL)
Deletion 8p FGFR-1 by FISH Test for Chronic Disease Monitoring
This test is not only used for initial diagnosis. It may be repeated to monitor residual disease in patients who have received chemotherapy or undergone a stem cell transplant. Doctors use it to assess whether the treatment has cleared the abnormal cells or whether any residual clones remain.
How to Prepare and What to Expect
The Deletion 8p FGFR-1 by FISH test requires samples from both bone marrow and peripheral blood. Understanding what to expect will help you feel at ease before your appointment.
Do You Need to Fast?
No fasting is required for this test under normal circumstances. However, if your doctor plans to use a sedative during the bone marrow collection procedure, they may ask you to avoid food and fluids for a period beforehand. Follow your doctor's specific instructions.
Practical Tips Before Your Test
Keep the following in mind before your appointment:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Inform your doctor about all medications you currently take, particularly blood thinners, as some may need to be paused before the procedure
- Do not stop any medication on your own without your doctor's guidance
- Tell your doctor if you have a bleeding disorder
- Arrange for someone to take you home if sedation is being used
- Wear comfortable clothing that gives easy access to the hip area
Step-by-Step Procedure
This test requires two sample types: bone marrow aspirate and peripheral blood. Here is what the collection process involves.
Bone Marrow Sample:
- You will lie on your side or stomach on the examination table.
- The skin over the back of the hip bone (posterior iliac crest) is cleaned with an antiseptic solution.
- A local anaesthetic is injected to numb the area before the procedure begins.
- Once the bone marrow cavity is accessed, a syringe is attached to draw out a small aspirate sample of approximately 3 ml.
- The sample is collected into a sodium heparin (green-top) tube and stored refrigerated at 2 to 8 degrees Celsius.
Peripheral Blood Sample:
- A small blood sample of 3 ml is drawn from a vein, usually in the arm, into an EDTA (lavender-top) tube and stored refrigerated alongside the bone marrow sample.
- Both samples are dispatched to the laboratory for FISH hybridisation and analysis.
Factors That Can Affect Accuracy
The following factors may influence the reliability of your test results:
- Poor sample quality or low cell viability at the time of collection
- Delays in transit that reduce specimen viability
- Freezing the specimen (samples must not be frozen)
- Genetic mutations too small to be detected by FISH technology
- Chromosomal changes in regions not targeted by the FISH probes used
Understanding Your Deletion 8p FGFR-1 by FISH Test Results
Results from the Deletion 8p FGFR-1 by FISH test are reported based on the signal pattern observed in the cells examined. A qualified haematologist or pathologist will review the findings.
| Result | Signal Pattern | Interpretation |
|---|---|---|
| Negative (Normal) | Two green-orange colocalisation signals (2GO) | FGFR1 gene is intact; no rearrangement detected |
| Positive (Abnormal) | One fused signal plus one separate green and one separate orange signal (1GO + 1G + 1O) | FGFR1 rearrangement detected |
A result is generally considered positive when more than 15% of cells examined show a split signal pattern.
A positive result is associated with 8p11 myeloproliferative syndrome, a rare and aggressive blood cancer. It may also indicate a risk of progression to acute leukaemia. A negative result does not entirely rule out a blood disorder, and your doctor may recommend additional genetic or molecular tests for a full evaluation.
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
How to Maintain Healthy Levels
Because this is a diagnostic test for genetic changes in blood cells, lifestyle measures cannot prevent chromosomal rearrangements. The following general tips support overall blood health:
- Attend regular health check-ups so that blood abnormalities are detected early
- If you have been diagnosed with a myeloproliferative disorder, stay in close contact with your haematologist and attend all follow-up appointments
- Eat a balanced diet and maintain good general health to support your body during diagnosis and treatment
Lupin Diagnostics Deletion 8p FGFR-1 by FISH Test Price
The Deletion 8p FGFR-1 by FISH test cost at Lupin Diagnostics starts at ₹4,700. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 4700 |
| CHENNAI | 4700 |
| HYDERABAD | 4700 |
| KOLKATA | 4700 |
| NAVI MUMBAI | 4700 |
| PUNE | 4700 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book the Deletion 8p FGFR-1 by FISH test online:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The Deletion 8p FGFR-1 by FISH test detects rearrangements or deletions in the FGFR1 gene on chromosome 8p11 using fluorescence in situ hybridisation technology. It is a specialised test used to diagnose rare blood cancers known as myeloid and lymphoid neoplasms with FGFR1 abnormalities. Your doctor will refer you for this test based on your symptoms and blood test findings.
This test is ordered when blood tests show unexplained high white blood cell counts or elevated eosinophils (a type of white blood cell), or when a myeloproliferative neoplasm or lymphoma is suspected. It may also be ordered to confirm a diagnosis of acute leukaemia with possible FGFR1 involvement.
Two samples are required: a bone marrow aspirate of 3 ml collected into a sodium heparin tube, and a peripheral blood sample of 3 ml collected into an EDTA tube. Both samples must be refrigerated and transported promptly to the laboratory.
The area is numbed with a local anaesthetic before the procedure, so most patients feel pressure rather than sharp pain. A brief, sharp sensation may occur during the aspiration step. Sedation is available if additional comfort is needed; if sedation is used, arrange for someone to take you home afterwards.
The report for the Deletion 8p FGFR-1 by FISH test is delivered within 8 days of sample collection at Lupin Diagnostics. FISH analysis is a detailed process that requires careful laboratory processing, which is why the turnaround time is longer than routine blood tests.
A positive result means that an FGFR1 gene rearrangement has been detected. This is associated with rare and aggressive blood cancers, including 8p11 myeloproliferative syndrome, which can progress to acute leukaemia. Your haematologist will discuss the clinical significance and next steps based on your full medical picture.
No. The Deletion 8p FGFR-1 by FISH test requires bone marrow aspiration, which must be performed at a diagnostic centre or hospital by trained medical professionals. Home collection is not available for this test. Please book an appointment at your nearest Lupin Diagnostics centre.
Deletion 8p FGFR-1 by FISH Test: Booking, Price, and Results
