Deletion 13q Multiple Myeloma, CLL Test
About Deletion 13q Multiple Myeloma, CLL Test
| Field | Value |
|---|---|
| Also Known As | Del(13q) FISH Test, 13q14 Deletion Test, Chromosome 13q Deletion Analysis, 13q- by FISH |
| Sample Type | Bone marrow aspirate (preferred) or peripheral blood |
| Fasting Required | Not required for the test itself. If sedation is used for bone marrow collection, fasting the night before may be advised. |
| Report Time | 3 Days |
| Recommended For | Adults diagnosed with or suspected of having multiple myeloma or chronic lymphocytic leukaemia |
| Price | Starting at ₹4,700 |
What Is a Deletion 13q Multiple Myeloma, CLL Test?
The Deletion 13q Multiple Myeloma, CLL test detects missing genetic material on chromosome 13, specifically at position 13q14. It uses a technique called FISH (fluorescence in situ hybridisation), which applies fluorescent probes to identify chromosomal changes in cells. Doctors order this test primarily to help diagnose, assess prognosis, and guide treatment decisions in patients with multiple myeloma or chronic lymphocytic leukaemia (CLL). It is also known as the Del(13q) FISH Test or the 13q14 Deletion Test.
What Does a Deletion 13q Multiple Myeloma, CLL Test Measure?
The Deletion 13q Multiple Myeloma, CLL test procedure analyses specific regions of chromosome 13 using FISH on bone marrow or peripheral blood cells. The key targets are listed below.
| Target Region | What It Represents |
|---|---|
| 13q14 locus (D13S319) | The primary site assessed; loss here is the most common chromosomal change in CLL |
| RB1 gene region | Location of the retinoblastoma-1 tumour suppressor gene at 13q14 |
| DLEU2/MIR15A/MIR16-1 locus | A tumour suppressor region whose deletion disrupts regulation of B-lymphocyte cell death |
The result is reported as either "not detected" (no deletion found) or "detected" (deletion present), along with the percentage of affected cells.
Why Is a Deletion 13q Multiple Myeloma, CLL Test Done?
This test is requested when a doctor suspects or has diagnosed a blood cancer and needs genetic information to assess the disease. It plays a key role in both initial diagnosis and ongoing monitoring.
Common Symptoms That May Require This Test
Your doctor may recommend this test if you are experiencing symptoms associated with multiple myeloma or CLL. These include:
- Persistent bone pain, especially in the back or ribs
- Unexplained fatigue or weakness
- Frequent infections
- Enlarged lymph nodes in the neck, armpits, or groin
- Easy bruising or bleeding
- Breathlessness or symptoms of anaemia (low red blood cell count)
- Numbness or tingling in the arms or legs
Conditions This Test Can Help Detect
This test helps identify or confirm the following conditions:
- Multiple myeloma (MM), a cancer of plasma cells in the bone marrow
- Chronic lymphocytic leukaemia (CLL), a cancer of white blood cells
- Monoclonal gammopathy of undetermined significance (MGUS) and smouldering multiple myeloma (SMM), for risk stratification
Deletion 13q Multiple Myeloma, CLL Test for Chronic Disease Monitoring
This test is used at initial diagnosis and again at disease progression or relapse. In multiple myeloma, it helps stratify patients into risk groups and guides treatment selection. In CLL, it is repeated as clinically indicated to track disease status and assist in planning ongoing management.
How to Prepare and What to Expect
Preparation is straightforward for most patients, but a few practical steps will help ensure accurate results and a smooth experience.
Do You Need to Fast?
Fasting is not required for this test. However, if your doctor plans to use sedation during the bone marrow aspiration, you may be asked to avoid food and drink from the night before. Confirm this with your doctor in advance.
Practical Tips Before Your Test
The following steps will help you prepare:
- Bring a detailed clinical history including your symptoms, previous test results, and any prior diagnosis, as this is required for the test
- Inform your doctor about all current medications, especially blood thinners (anticoagulants) and any history of bleeding disorders
- Arrange for someone to drive you home if sedation is being used
- Wear loose, comfortable clothing to allow easy access to the collection site
- Plan to rest after the procedure, as you may feel drowsy if a sedative is given
Step-by-Step Procedure
Two sample types are required: a bone marrow aspirate and a peripheral blood sample.
Bone Marrow Sample Collection:
- You will lie on your side or stomach on an examination table. The area at the back of the hip bone (iliac crest) is cleaned and numbed with a local anaesthetic.
- A small incision is made in the skin and a hollow needle is inserted into the bone.
- A syringe attached to the needle withdraws a small amount of liquid bone marrow. You may feel brief pressure during this step.
- The sample is collected into a sodium heparin (green) tube and stored at 2 to 8°C for transport.
Peripheral Blood Sample Collection:
- A separate blood sample of 3 mL is drawn from a vein, usually in the arm, and collected into an EDTA (lavender) tube.
- Both samples are labelled and sent to the laboratory, ideally within 24 hours of collection, for FISH analysis.
Factors That Can Affect Accuracy
The following factors may influence the reliability of your results:
- Delay in transporting samples beyond 24 hours of collection
- A low number of plasma cells in the bone marrow sample
- Inadequate sample volume or improper handling during transport
- Presence of other concurrent chromosomal abnormalities
Understanding Your Deletion 13q Multiple Myeloma, CLL Test Results
Results are interpreted by a specialist in the context of your full clinical picture. The table below outlines what each outcome generally indicates.
| Parameter | Result | Interpretation |
|---|---|---|
| 13q14 deletion | Not detected (Negative) | No deletion found; the chromosomal region appears intact |
| 13q14 deletion | Detected (Positive) | Deletion identified; percentage of affected cells is reported |
In CLL, a deletion at 13q14 detected as the sole abnormality is generally a favourable prognostic marker. In multiple myeloma, the same deletion is considered a significant adverse prognostic factor, particularly when detected by standard cytogenetics.
"These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice."
Results During Special Conditions
The percentage of cells carrying the deletion matters in CLL. Patients with a higher proportion of 13q-deleted cells may follow a more aggressive clinical course, even when the deletion appears as the only chromosomal change. Your haematologist will account for this when advising on treatment.
How to Maintain Healthy Levels
While genetic test results cannot be changed through lifestyle alone, the following steps support your overall wellbeing during monitoring:
- Attend all follow-up appointments recommended by your haematologist (blood specialist)
- Report any new or worsening symptoms promptly, such as unexplained fatigue, bone pain, or recurring infections
- Support general health through balanced nutrition and adequate rest
Lupin Diagnostics Deletion 13q Multiple Myeloma, CLL Test Price
The Deletion 13q Multiple Myeloma, CLL test cost at Lupin Diagnostics starts at ₹4,700. This test requires a visit to a Lupin Diagnostics centre, as home collection is not available for this specialised cytogenetic test.
| City | Approximate Price (₹) |
|---|---|
| Mumbai | 4700 |
| Pune | 4700 |
| Bangalore | 4700 |
| Chennai | 4700 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your Deletion 13q Multiple Myeloma, CLL test online:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within 3 days.
Frequently Asked Questions
This test detects the loss of genetic material at position 13q14 on chromosome 13. It is used to help diagnose multiple myeloma and chronic lymphocytic leukaemia, assess prognosis, and guide treatment decisions. It may also be used to monitor disease progression or relapse over time.
Two samples are required: a bone marrow aspirate collected in a sodium heparin tube and a peripheral blood sample collected in an EDTA tube. Both are sent to the laboratory for FISH analysis.
You may feel brief pressure or discomfort during the procedure. A local anaesthetic is applied to numb the area beforehand, and sedation may be offered for additional comfort. Most patients tolerate the procedure well.
In CLL, a Deletion 13q found as the sole chromosomal abnormality is generally considered a favourable prognostic marker, often associated with slower disease progression. However, a higher percentage of affected cells may indicate a more active disease course.
In multiple myeloma, deletion of chromosome 13q is an adverse prognostic factor. Its significance depends on how it is detected and whether other chromosomal changes are present. Your haematologist will discuss what the finding means for your specific situation.
The Deletion 13q Multiple Myeloma, CLL test is a specialised cytogenetic investigation. Clinical context, including your symptoms, prior diagnoses, and previous test results, helps the laboratory and reporting doctor interpret the FISH findings accurately in relation to your overall condition.
Yes, you can complete your Deletion 13q Multiple Myeloma, CLL test online booking through the Lupin Diagnostics website. As this test requires specialised sample collection, you will need to visit a Lupin Diagnostics centre rather than opt for home collection.
Deletion 13q Multiple Myeloma, CLL Test
