Cytoplasmic MPO by Flowcytometry Test: Booking, Price, and Results
About Cytoplasmic MPO by Flowcytometry Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | Cytoplasmic Myeloperoxidase by Flow Cytometry, cMPO Flow Cytometry, Intracellular MPO Staining, MPO Immunophenotyping |
| Sample Type | Bone marrow aspirate (collected in sodium heparin and EDTA tubes) |
| Fasting Required | No fasting required |
| Report Time | 3 days |
| Recommended For | All ages and genders; primarily patients with suspected acute leukaemia |
| Price | Starting at ₹2,300 |
What Is a Cytoplasmic MPO by Flowcytometry Test?
The Cytoplasmic MPO by Flowcytometry test detects the presence of myeloperoxidase (MPO), an iron-containing protein found inside certain white blood cells, using a technique called flow cytometry. It is primarily ordered when a doctor suspects acute leukaemia and needs to identify the type of leukaemic cells. The test is also known as Cytoplasmic Myeloperoxidase by Flow Cytometry or cMPO Flow Cytometry. A bone marrow sample is used for analysis.
What Does a Cytoplasmic MPO by Flowcytometry Test Measure?
This test analyses the expression of myeloperoxidase inside cells obtained from a bone marrow sample. The following aspects are evaluated during the test:
| Parameter | What It Indicates |
|---|---|
| MPO positivity in blast cells | Whether leukaemic cells carry a myeloid (granulocyte-related) identity |
| MPO fluorescence intensity | Distinguishes granulocytes (high), monocytes (intermediate), and lymphocytes (low or absent) |
| Percentage of MPO-positive cells | Determines myeloid lineage assignment using defined positivity thresholds |
A key threshold used in flow cytometry is 10% or more of blast cells showing MPO positivity, which points to myeloid lineage. In cytochemistry, a lower threshold of 3% is used. Under some standardised protocols, a threshold of 20% or more offers the best diagnostic accuracy.
Why Is a Cytoplasmic MPO by Flowcytometry Test Done?
Doctors order this test to classify acute leukaemia and guide treatment decisions. Below are the common reasons a doctor may recommend it.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to investigate a possible blood disorder using this test:
- Unexplained fatigue or persistent weakness
- Pale skin, possibly due to anaemia (low red blood cell count)
- Mild fever or recurring chills
- Frequent or difficult-to-clear infections
- Nosebleeds or bleeding from the gums
- Easy bruising without a clear cause
- Shortness of breath during light physical activity
Conditions This Test Can Help Detect
This test helps identify or classify several blood disorders. It may be ordered when any of the following are suspected:
- Acute myeloid leukaemia (AML): MPO is a reliable marker for myeloid lineage assignment in leukaemic blast cells
- Mixed phenotype acute leukaemia (MPAL): MPO staining helps confirm myeloid involvement when cells show both lymphoid and myeloid features
- Differentiation of AML from acute lymphoblastic leukaemia (ALL): helps distinguish between the two subtypes based on MPO expression
- Myelodysplastic syndromes (MDS): flow cytometric MPO analysis in blood cells can assist in evaluation
- Myeloperoxidase deficiency: a rare immune disorder marked by reduced or absent MPO activity in neutrophils and monocytes
How to Prepare and What to Expect
No special preparation is needed before this test, but a few simple steps can help the process go smoothly.
Do You Need to Fast?
No, fasting is not required before this test. You may eat and drink normally. There are no water or dietary restrictions before sample collection.
Practical Tips Before Your Test
The following steps can help ensure a smooth experience on the day of your test:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test.
- Stay well hydrated before your appointment.
- Inform the doctor or technician about all medications, supplements, or therapies you are currently taking.
- Mention if you have recently received chemotherapy or immunosuppressive treatment.
- Carry any previous haematology or blood disorder reports for reference.
Step-by-Step Procedure
Bone marrow collection for this test follows a defined process. Here is what typically happens:
- A doctor or trained specialist will explain the bone marrow aspiration procedure to you and answer any questions.
- The skin over the posterior iliac crest (the back of the hip bone) is cleaned, and a local anaesthetic is applied to numb the area.
- A thin needle is inserted to withdraw a small liquid sample of bone marrow. This procedure is called bone marrow aspiration.
- The aspirate is collected into two separate tubes: one sodium heparin (green cap) tube and one EDTA (lavender cap) tube, each requiring approximately 3 mL of sample.
- The sample is stored at refrigerator temperature (2 to 8 degrees Celsius) and dispatched to the laboratory promptly for processing within 24 hours.
- In the laboratory, the sample undergoes fixation and permeabilisation before flow cytometry analysis. Your report is typically ready within 3 days.
Factors That Can Affect Accuracy
Several factors may affect how reliable the test results are:
- Sample freshness, if not ideally delivered to the lab within 24 hours of collection
- Proper fixation and permeabilisation technique during sample processing
- Correct antibody clone selection for MPO staining
- Laboratory instrument calibration
- Use of the correct anticoagulant tube (EDTA or sodium heparin as specified)
- Recent chemotherapy or immunosuppressive therapy, which may alter cell populations
Understanding Your Cytoplasmic MPO by Flowcytometry Test Results
Results from this test require interpretation by a qualified haematologist or pathologist alongside your full clinical picture. The table below outlines general reference thresholds:
| Parameter | Threshold / Expected Pattern |
|---|---|
| MPO positivity (flow cytometry) | 10% or more of blast cells positive indicates myeloid lineage |
| MPO positivity (cytochemistry) | 3% or more of blast cells positive |
| Granulocytes | Bright (high) MPO expression |
| Monocytes | Intermediate MPO expression |
| Lymphocytes / normal lymphoblasts | Minimal or absent MPO expression |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
A positive or high MPO result may suggest acute myeloid leukaemia, mixed phenotype acute leukaemia, or myeloid lineage assignment in ambiguous acute leukaemia. A low or absent MPO result may point towards acute lymphoblastic leukaemia, myeloperoxidase deficiency, or T-cell lineage leukaemia.
Results During Special Conditions
Certain underlying conditions can affect MPO expression and should be considered during interpretation:
- Secondary MPO deficiency may develop due to conditions such as diabetes mellitus, iron deficiency, severe infections, lead poisoning, renal transplantation, and the use of certain cytotoxic agents. Unlike the hereditary form, the acquired deficiency is usually partial and often temporary, resolving once the underlying condition is treated.
How to Maintain Healthy Levels
Since this is a diagnostic test for blood disorders rather than a routine wellness marker, the concept of "maintaining levels" does not apply in the usual sense. The following general tips support overall blood health:
- Attend regular medical check-ups, especially if you have a family history of blood disorders.
- Follow your haematologist's guidance on overall health management.
- Report any new or recurring symptoms such as unusual fatigue, unexplained bruising, or persistent fever to your doctor promptly.
Lupin Diagnostics Cytoplasmic MPO by Flowcytometry Test Price
The Cytoplasmic MPO by Flowcytometry test cost at Lupin Diagnostics starts at ₹2,300. This test requires a visit to a Lupin Diagnostics centre as home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 2300 |
| CHENNAI | 2300 |
| HYDERABAD | 2300 |
| KOLKATA | 2300 |
| NAVI MUMBAI | 2300 |
| PUNE | 2300 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Booking your Cytoplasmic MPO by Flowcytometry test online at Lupin Diagnostics is straightforward:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within 3 days of sample collection.
Frequently Asked Questions
This test is used to detect myeloperoxidase inside leukaemic cells to determine whether they are of myeloid origin. It is one of the most specific tools available for classifying acute leukaemia and helps doctors decide on the appropriate course of treatment.
A bone marrow sample is collected through a bone marrow aspiration procedure. The sample is collected at a Lupin Diagnostics centre by a trained specialist and placed into two types of tubes for analysis.
No special preparation, such as fasting, is needed. However, you should bring a detailed clinical history, including any previous blood test reports, and inform the doctor about all current medications and recent therapies before the procedure.
A positive result means that 10% or more of the blast cells (immature blood cells) express myeloperoxidase, which indicates the cells are of myeloid origin. This supports a diagnosis of acute myeloid leukaemia or a related myeloid lineage disorder. Your haematologist will confirm the diagnosis using all available clinical and laboratory findings.
Yes. Certain acquired conditions, such as diabetes mellitus, severe infections, iron deficiency, and the use of cytotoxic drugs, may reduce MPO expression in neutrophils. This acquired form of MPO deficiency is usually partial and often resolves when the underlying condition is treated.
MPO expression levels vary depending on a patient's diagnosis, treatment history, and overall health. Providing a complete clinical history allows the laboratory specialist to interpret results accurately and in the right context, which is especially important for a specialised test like this one.
Cytoplasmic MPO by Flowcytometry Test: Booking, Price, and Results
