CD8, Flowcytometry Test: Booking, Price, and Results
About CD8, Flowcytometry Test
| Field | Value |
|---|---|
| Also Known As | CD8 T-Cell Count Test, CD8+ T Lymphocyte Count Test, Cytotoxic T-Cell Count Test, T-Suppressor Cell Count Test |
| Sample Type | Whole blood (EDTA tube) or bone marrow aspirate |
| Fasting Required | Not required |
| Report Time | 1 day |
| Recommended For | All genders and age groups |
| Price | Starting at ₹1,300 |
What is a CD8, Flowcytometry Test?
The CD8, flowcytometry test measures the number and proportion of CD8+ T cells, a type of immune cell that targets and destroys infected or abnormal cells in the body. The test uses a laboratory technique called flow cytometry, which analyses thousands of cells rapidly and with high accuracy. Doctors commonly order this test to assess immune function, monitor HIV treatment, or investigate blood disorders. It is also known as the CD8 T-cell count test, CD8+ T lymphocyte count test, cytotoxic T-cell count test, or T-suppressor cell count test. This test requires whole blood and bone marrow samples.
What Does a CD8, Flowcytometry Test Measure?
This test analyses specific immune cells in your blood or bone marrow. The following parameters are typically reported:
| Parameter | What It Tells Your Doctor |
|---|---|
| CD8+ T cells (absolute count) | The total number of cytotoxic T cells per microlitre of blood |
| CD8+ T cells (%) | The share of CD8+ T cells among all lymphocytes (infection-fighting white blood cells) |
| CD4:CD8 ratio | A comparison of helper T cells to cytotoxic T cells, reflecting overall immune balance |
Why is a CD8, Flowcytometry Test Done?
A doctor may request the CD8, flowcytometry test for several reasons, ranging from routine immune monitoring to investigating a suspected blood disorder.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to order this test:
- Frequent or recurring infections that do not resolve easily
- Unexplained fatigue lasting several weeks
- Swollen lymph nodes in the neck, armpits, or groin
- Persistent fever without a clear cause
- Unexplained weight loss
- Night sweats
- Chronic diarrhoea
Conditions This Test Can Help Detect
This test can help identify or monitor several conditions, including:
- HIV infection, where CD4 cells fall and CD8 cells may rise, affecting the CD4:CD8 ratio
- Post-transplant immune recovery following bone marrow transplant or immunotherapy
- Autoimmune diseases and chronic viral infections
- Leukaemia, where flow cytometry can identify and classify abnormal cell populations
- Primary immunodeficiency, including CD8 deficiency, which may cause recurrent respiratory infections
CD8, Flowcytometry Test for Chronic Disease Monitoring
In patients living with HIV or AIDS, CD8 cell counts are typically checked every three to six months to track disease progression and gauge how well antiretroviral therapy (ART) is working. The test is equally useful for patients receiving immunosuppressive therapy, helping doctors monitor immune recovery over time.
How to Prepare and What to Expect
Preparing for the CD8, flowcytometry test procedure is straightforward. Here is what you should know before your appointment.
Do You Need to Fast?
No, fasting is not required before this test. You may eat and drink as normal on the day of collection. Your doctor will advise if anything else needs to be adjusted based on your specific situation.
Practical Tips Before Your Test
Keep the following points in mind before attending your appointment:
- Bring a detailed clinical history, including your symptoms, previous test results, and any relevant medical records, as this is required for the test.
- Inform the staff about all medications, supplements, or conditions that may affect your immune system.
- Drink enough water beforehand to stay hydrated; this makes blood collection easier.
- Wear clothing with loose sleeves for easy access to the arm.
Step-by-Step Procedure
- A trained staff member will review your clinical history and confirm the sample type required, either a blood sample or a bone marrow sample.
- For a blood sample: A suitable vein in your arm is identified, the area is cleaned with an antiseptic, and a small sample of around 3 ml is drawn into a lavender-top EDTA tube.
- For a bone marrow sample: The procedure is done at a medical facility. A local anaesthetic is given, and light sedation may be offered. A hollow needle is inserted through the skin into the hip bone to collect the liquid bone marrow (aspirate), typically 3 ml, into a sodium heparin or EDTA tube.
- For both sample types, the collected sample is labelled carefully with your details.
- The sample is stored at a refrigerated temperature of 2 to 8°C and promptly dispatched to the laboratory.
- The sample is analysed using the flow cytometry method, and your report is prepared within 1 day.
Factors That Can Affect Accuracy
The following factors may influence the reliability of your test results:
- Delays between sample collection and laboratory processing
- Incorrect storage or transport temperature
- Recent infections or vaccinations
- Medications that suppress or stimulate the immune system
Understanding Your CD8, Flowcytometry Test Results
Results from the CD8, flowcytometry test should always be reviewed alongside your clinical history. Below are the general reference ranges for healthy adults:
| Parameter | Normal Range | Unit |
|---|---|---|
| CD8+ T cells (absolute count) | 150 to 1,000 | cells/mm³ |
| CD8+ T cells (%) | 15 to 40 | % of lymphocytes |
| CD4:CD8 ratio | 0.5 to 2.5 | ratio |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain conditions can shift CD8+ T-cell counts in specific ways:
- In older adults, prolonged exposure to various antigens over a lifetime may cause CD8+ T cells to shift towards a less active state, contributing to low-grade inflammation linked to age-related health changes.
- In autoimmune conditions, CD8+ T cells may become overactive and mistakenly attack the body's own tissues.
- In cancer or chronic viral infections, CD8+ T cells can progressively lose their ability to fight effectively, a state known as T-cell exhaustion.
How to Maintain Healthy Levels
These general wellness habits support a balanced immune system:
- Eat a varied diet with adequate vitamins and minerals, particularly vitamins C, D, and zinc
- Aim for regular, quality sleep and manage daily stress through relaxation or light exercise
- Avoid smoking and limit alcohol, both of which can impair immune function over time
Lupin Diagnostics CD8, Flowcytometry Test Price
The CD8, flowcytometry test cost at Lupin Diagnostics starts at ₹1,300. This test requires a visit to a medical facility; home collection is not available. The table below shows indicative prices:
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1300 |
| CHENNAI | 1300 |
| HYDERABAD | 1300 |
| KOLKATA | 1300 |
| NAVI MUMBAI | 1300 |
| PUNE | 1300 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
What is the CD8, flowcytometry test used for?
This test measures the number and proportion of CD8+ T cells (cytotoxic T cells) in the blood or bone marrow. It is used to assess immune health, monitor HIV disease progression and treatment response, investigate blood cancers such as leukaemia, and evaluate conditions affecting the immune system.
Do I need to fast before the CD8, flowcytometry test?
No fasting is needed. You can eat and drink normally before the test. If your doctor has ordered additional tests alongside this one, check whether any of those have separate fasting requirements.
What sample is collected for this test?
Depending on what your doctor needs to assess, the sample may be venous whole blood or a bone marrow aspirate. For routine immune monitoring, a blood sample from a vein in the arm is usually sufficient. Bone marrow collection is done under local anaesthetic when a more detailed assessment is required.
How long does it take to get the results?
The report is typically ready within 1 day of sample collection at Lupin Diagnostics. The results are delivered directly via email or WhatsApp.
What does a low CD4:CD8 ratio indicate?
A ratio below 1.0 may indicate HIV infection or another condition causing immune suppression. This result alone is not a diagnosis. Your doctor will consider it alongside other test findings and your clinical history before drawing any conclusions.
Can the CD8, flowcytometry test diagnose HIV?
No, this test does not diagnose HIV. It monitors immune status by measuring T-cell levels. A separate HIV antibody or antigen test is required for diagnosis. In people already known to have HIV, the CD8, flowcytometry test is used to track disease progression and how well treatment is working.
CD8, Flowcytometry Test: Booking, Price, and Results
