CD3, Flow Cytometry Test: Booking, Price, and Results
About CD3, Flow Cytometry Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD3 Total T-Lymphocytes Test, Pan T-Cell Marker Test, T Cell Count, CD3+ T-Cell Count |
| Sample Type | Bone marrow and whole blood (EDTA tube) |
| Fasting Required | No |
| Report Time | 3 days |
| Recommended For | Men and women of all age groups |
| Price | Starting at ₹2,300 |
What is a CD3, Flow Cytometry Test?
The CD3, flow cytometry test measures the number and proportion of T lymphocytes (a key type of immune cell) in the blood and bone marrow. It uses a laboratory technique called flow cytometry to count cells carrying the CD3 protein marker, which is present on the surface of all mature T cells. Doctors order this test to assess immune system function, investigate suspected immune disorders, and monitor certain cancers. It is also known as the CD3 Total T-Lymphocytes Test or T-Cell Count.
What Does a CD3, Flow Cytometry Test Measure?
The CD3, flow cytometry test procedure analyses two key values from your sample. These are described below.
| Parameter | What It Tells You |
|---|---|
| CD3+ T Cells (Absolute Count) | The total number of CD3-positive T cells per microlitre of blood, giving a direct measure of T-cell quantity |
| CD3+ T Cells (Percentage) | The proportion of lymphocytes (white blood cells) that carry the CD3 marker, reflecting the relative size of the T-cell population |
Because every mature T cell carries the CD3 marker, both values together provide a clear picture of how well the cellular arm of the immune system is functioning.
Why is a CD3, Flow Cytometry Test Done?
Doctors request the CD3, flow cytometry test for a range of clinical reasons, from initial investigation of immune symptoms to ongoing monitoring of known conditions.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to order this test:
- Frequent or recurring infections that are difficult to resolve
- Unexplained fatigue or prolonged recovery from illness
- Fever and night sweats without a clear cause
- Enlarged lymph nodes (swollen glands in the neck, armpits, or groin)
- Unexplained weight loss
- Joint pain or skin rashes suggestive of an autoimmune condition
- Inflammatory symptoms that do not respond to standard treatment
Conditions This Test Can Help Detect
The test can support the diagnosis or monitoring of several conditions, including:
- Autoimmune diseases, where the immune system mistakenly attacks the body's own healthy tissue
- Immunodeficiency disorders, where the immune system is weaker than normal
- HIV infection and its progression
- Certain blood cancers, including leukaemias and lymphomas
- Severe combined immunodeficiency (SCID), a serious inherited immune disorder seen in infants
- T-lymphoblastic leukaemia or lymphoma, a cancer of immature T cells
CD3, Flow Cytometry Test for Chronic Disease Monitoring
This test is regularly used to track immune function over time in patients with chronic conditions. For people living with HIV, it may be recommended every 3 to 6 months to monitor disease progression and gauge the effectiveness of treatment. CD3+ T-cell counts are also monitored in organ transplant recipients to evaluate whether immunosuppressive therapy is working as intended.
How to Prepare and What to Expect
Preparation for the CD3, flow cytometry test is straightforward, but a few steps will help ensure accurate results.
Do You Need to Fast?
No, fasting is not required before this test. You may eat and drink normally unless your doctor has given you different instructions.
Practical Tips Before Your Test
Keep the following points in mind before your appointment:
- Bring a detailed clinical history file, including your symptoms, previous test results, and relevant family history, as this is required for the test
- Inform your doctor of all medications and supplements you are currently taking, particularly immunosuppressants, steroids, or chemotherapy agents
- If you are on immunosuppressive therapy, your doctor may advise you to have the sample drawn before your next dose
- Avoid strenuous physical activity in the hours leading up to sample collection
- Try to schedule the test during a period when you do not have an active infection, as temporary immune changes can affect T-cell counts
- Stay well hydrated before your appointment
Step-by-Step Procedure
This test requires two types of samples: a bone marrow sample and a whole blood sample. While the whole blood sample can be collected anywhere, the bone marrow sample will have to be collected at the diagnostic centre.
Bone Marrow Sample Collection:
- A doctor or trained specialist will clean and numb a small area, usually over the hip bone, before the procedure.
- A fine needle is inserted into the bone marrow cavity to withdraw a small bone marrow sample into a sodium heparin (green top) or EDTA (lavender top) tube.
- The site is cleaned and covered once the sample is collected. You may feel mild pressure or brief discomfort during this step.
Whole Blood Sample Collection:
- A small blood sample (approximately 3 ml) is drawn from a vein in your arm using a sterile needle and collected into a lavender-top EDTA tube (a tube containing a special anticoagulant to prevent clotting).
- Both samples are labelled and stored under refrigeration (2°C to 8°C) to preserve their integrity during transport.
- In the laboratory, both samples are processed using flow cytometry, a technique in which cells are tagged with fluorescent markers and passed through a laser-based analyser to count and characterise T cells precisely.
Factors That Can Affect Accuracy
Several factors can influence your results. These include:
- Active infections or recent vaccinations, which can temporarily alter T-cell counts
- Immunosuppressive medications, including corticosteroids, chemotherapy, and biologic agents
- Recent surgery or significant physical or emotional stress
- Strenuous exercise close to the time of collection
- Circadian rhythm variations (the natural fluctuation of cell counts across the day)
- Improper sample storage or delays in processing
Understanding Your CD3, Flow Cytometry Test Results
Your results reflect the state of your T-cell population at the time of testing. The table below shows general reference ranges for adults.
| Parameter | Normal Range | Unit |
|---|---|---|
| CD3+ T Cells (Absolute Count) | 900 to 2,500 | cells/µL |
| CD3+ T Cells (Percentage) | 60 to 85 | % of lymphocytes |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
A count within the normal range generally indicates a healthy T-cell population and adequate cellular immune function. A higher-than-normal count may be associated with active infections, inflammation, autoimmune conditions, or certain blood cancers. A lower-than-normal count may point to advanced HIV infection, inherited immune deficiency, the effects of chemotherapy or radiation, bone marrow failure, or long-term use of immunosuppressive medicines.
Results During Special Conditions
Certain situations can alter your CD3 results independently of any underlying disease:
- Medications such as corticosteroids, chemotherapy agents, and biologic immunosuppressants often reduce the number of circulating T cells. Some antivirals and growth factors may also affect counts.
- Recent surgery or periods of intense stress can temporarily shift T-cell levels.
- If samples are stored at room temperature for extended periods, CD3 cell percentages may change, which is why prompt processing is important.
How to Maintain Healthy Levels
While medical management is your doctor's responsibility, the following general wellness habits support immune health:
- Eat a balanced diet that includes adequate protein, fresh fruits, and vegetables to provide the nutrients your immune cells need.
- Engage in regular moderate exercise, which is associated with better T-cell function and immune responsiveness.
- Prioritise restful sleep and find ways to manage chronic stress, as both have a direct effect on immune cell levels.
Lupin Diagnostics CD3, Flow Cytometry Test Price and Home Collection
The CD3, flow cytometry test is available at Lupin Diagnostics starting at ₹2,300, with home collection available in select cities. The test is classified as a super-specialised investigation, and appointments can be scheduled online.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 2300 |
| CHENNAI | 2300 |
| HYDERABAD | 2300 |
| KOLKATA | 2300 |
| NAVI MUMBAI | 2300 |
| PUNE | 2300 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
- Select the CD3, flow cytometry test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within 3 days of sample collection.
Home Collection
Lupin Diagnostics offers home collection for the CD3, flow cytometry test, along with online booking across multiple cities for tests that require a whole blood sample. For those tests that need a bone marrow aspirate, you will have to visit the diagnostic centre or a clinic. All samples are processed in NABL-accredited laboratories by experienced specialists. Your digital report is delivered securely via email or WhatsApp once ready.
Frequently Asked Questions
This test measures the total count of T lymphocytes in the blood and bone marrow. Doctors use it to investigate immune system disorders, monitor conditions such as HIV infection, and support the diagnosis of certain blood cancers. It gives a clear picture of whether the cellular immune system is functioning within a healthy range.
No, fasting is not required. You can eat and drink normally before your appointment. Follow your routine unless your doctor has specifically advised otherwise.
The CD3, flow cytometry test price at Lupin Diagnostics starts at ₹2,300. The final cost may vary depending on your city and the location of the collection centre. Please confirm the price at the time of booking.
Because CD3 T-cell counts are influenced by many factors, including medications, past illnesses, and underlying conditions, your clinical history helps the laboratory and your doctor interpret the results accurately. Bring details of your current symptoms, previous test reports, and any medications you are taking.
Yes. Immunosuppressants, corticosteroids, chemotherapy agents, and certain biologic drugs can significantly lower T-cell counts. Your doctor may advise you on the best timing for the test relative to your medication schedule to get the most reliable result.
Reports for the CD3, flow cytometry test are typically ready within 3 days of sample collection. The bone marrow and blood samples need careful processing in a specialised laboratory, which accounts for the turnaround time.
If your results fall outside the normal range, your doctor may refer you to a haematologist (a specialist in blood disorders) or an immunologist (a specialist in immune system conditions). These specialists can carry out further investigations and guide appropriate next steps.
CD3, Flow Cytometry Test: Booking, Price, and Results
