CD2, Flow Cytometry Test: Booking, Price, and Results
About CD2, Flow Cytometry Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD2 Flow Cytometry, CD2 Immunophenotyping, T11 Marker, LFA-2 Marker |
| Sample Type | Bone marrow aspirate |
| Fasting Required | No |
| Report Time | 3 Days |
| Recommended For | Adults and children of all ages; both genders |
| Price | Starting at ₹2,300 |
What is a CD2, Flow Cytometry Test?
The CD2, Flow Cytometry test measures the presence and proportion of CD2-expressing T cells in a bone marrow sample. CD2 (also called T11 or LFA-2) is a protein found on the surface of T cells and natural killer (NK) cells, which are key players in the body's immune defence. Doctors order this test when they suspect certain blood cancers or immune system disorders. It is also known as CD2 Flow Cytometry or CD2 Immunophenotyping.
What Does a CD2, Flow Cytometry Test Measure?
The CD2 Flow Cytometry test examines a specific protein marker on immune cells and assesses how it is expressed across the cell population. The table below explains what is evaluated.
| Component | What It Means |
|---|---|
| CD2 antigen expression | The percentage of T cells displaying the CD2 protein on their surface |
| CD2+ T cell population | Identifies whether T cells are normal, increased, or showing abnormal patterns |
| Cell viability and count | Assesses the health and number of cells in the sample during analysis |
CD2 plays an important role in T-cell activation, immune signalling, and cell-to-cell communication. Abnormal expression patterns can point to underlying blood or immune conditions.
Why is a CD2, Flow Cytometry Test Done?
This test is ordered by haematologists and oncologists when there is a clinical suspicion of blood-related or immune system conditions. Below are the key reasons it may be requested.
Common Symptoms That May Require This Test
Several symptoms may prompt a doctor to request a CD2, Flow Cytometry test. These include:
- Painless swelling of lymph nodes in the neck, armpits, or groin
- Persistent, unexplained fever
- Drenching night sweats
- Unexplained weight loss
- Unusual fatigue not explained by other causes
- Skin rashes or lesions without a clear cause
- Frequent or recurring infections
Conditions This Test Can Help Detect
The CD2 Flow Cytometry test can assist in identifying several blood and immune conditions, including:
- T-lymphoblastic leukaemia and lymphoma (T-ALL/LBL), a rare form of acute blood cancer
- Mature T-cell neoplasms (cancers arising from fully developed T cells)
- T-cell lymphoma, a rare type of non-Hodgkin lymphoma
- Leukaemias and other haematological malignancies
- Autoimmune disorders, in which the immune system targets the body's own tissues
- Immunodeficiency conditions affecting T-cell function
CD2, Flow Cytometry Test for Chronic Disease Monitoring
For patients already undergoing treatment for conditions such as T-cell lymphoma or leukaemia, this test may be repeated at intervals to track how well the treatment is working. It helps evaluate disease progression and assess whether cell populations are responding as expected. The treating haematologist or oncologist will determine how often the test needs to be repeated.
How to Prepare and What to Expect
Preparation for this test is straightforward, but there are a few important steps to follow before your appointment.
Do You Need to Fast?
No, fasting is not required before the CD2, Flow Cytometry test. You may eat and drink normally before your visit.
Practical Tips Before Your Test
Following these steps will help ensure a smooth sample collection and accurate results:
- Bring your detailed clinical history, including your symptoms, previous test results, and any relevant medical records, as this is required for the test
- Inform your doctor about all medications and supplements you are currently taking, as some can affect immune cell counts
- Stay well hydrated before your appointment
- Wear comfortable clothing that does not restrict movement, as you will be visiting a centre for the procedure
Step-by-Step Procedure
The CD2, Flow Cytometry test procedure involves bone marrow sample collection, which is performed by a trained specialist at the diagnostic centre. Here is what to expect:
- You will be asked to lie in a specific position. The skin over the collection site (usually the hip bone) is cleaned, and a local anaesthetic is applied to numb the area.
- A specialist inserts a thin needle into the bone marrow cavity to withdraw a small sample of bone marrow fluid, collected into a sodium heparin (green-top) tube. A second sample may be taken in a lavender top EDTA tube.
- The sample is labelled and stored at 2 to 8 degrees Celsius to maintain cell integrity during transport to the laboratory.
- In the laboratory, the sample is treated with fluorescently labelled antibodies that attach to the CD2 protein on cell surfaces.
- The prepared sample is passed through a flow cytometer, which uses a laser beam to count and classify individual cells based on how light scatters off them.
- A pathologist reviews the data and prepares a detailed report, which is typically ready within 3 days.
Factors That Can Affect Accuracy
Certain factors may influence the reliability of your CD2, Flow Cytometry test results:
- Sample freshness: The sample should be processed within 48 hours of collection for best accuracy
- Improper storage or significant delays in transport can reduce cell viability
- Haemolysis (breakdown of red blood cells) in the sample can interfere with analysis
- Immunosuppressive medications may alter CD2 expression patterns
- Recent infections can temporarily raise CD2+ cell levels
Understanding Your CD2, Flow Cytometry Test Results
Your results will show the proportion of CD2-expressing T cells in the sample. A qualified haematologist or pathologist will interpret these values alongside your clinical history and other test findings.
| Parameter | Reference Range | Unit |
|---|---|---|
| CD2+ T cells | 59 to 92 | % of T cells |
Note: Reference ranges may differ slightly between laboratories. Values outside this range do not automatically indicate a serious condition. Results must always be reviewed in the context of your overall health.
"These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice."
Results During Special Conditions
Certain situations can affect how results are interpreted. Recent infections may temporarily raise CD2+ cell levels, which can complicate analysis. Immunosuppressive medications may lower CD2 expression, and this should be disclosed to your doctor before testing. Additionally, malignant T cells often show significantly different CD2 expression patterns compared to normal T cells, which is why clinical context and professional interpretation are essential.
How to Maintain Healthy Levels
While CD2 expression is primarily governed by your immune status and underlying conditions, general immune health can be supported through the following:
- Eat a nutrient-rich diet with adequate vitamins and minerals that support immune cell function
- Manage chronic stress through regular physical activity, adequate sleep, and relaxation practices
- Follow your treating doctor's guidance for managing any diagnosed conditions that affect immune cells
Lupin Diagnostics CD2, Flow Cytometry Test Price
The CD2, Flow Cytometry test cost at Lupin Diagnostics starts at ₹2,300. This test requires a visit to a Lupin Diagnostics centre, as home collection is not available due to the specialised nature of bone marrow sample collection and processing requirements.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 2300 |
| CHENNAI | 2300 |
| HYDERABAD | 2300 |
| KOLKATA | 2300 |
| NAVI MUMBAI | 2300 |
| PUNE | 2300 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Booking your CD2, Flow Cytometry test online is straightforward. Follow these steps:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within 3 days.
Frequently Asked Questions
This test is primarily used to help diagnose and monitor blood cancers and immune system disorders. It measures the proportion of T cells expressing the CD2 protein, which can indicate whether T-cell populations are normal or showing signs of malignancy. Doctors typically order it when investigating conditions such as T-cell lymphoma or leukaemia.
No fasting is required. You can eat and drink as usual before your appointment. However, you should bring a detailed clinical history, including previous test results and a record of any medications you are taking.
A bone marrow aspirate is collected during the CD2, Flow Cytometry test procedure. This is done by a trained specialist at the diagnostic centre using a needle inserted into the bone marrow, usually at the hip. A local anaesthetic is used to minimise discomfort.
Results are typically available within 3 days of sample collection at Lupin Diagnostics. The sample requires careful laboratory processing and expert review by a pathologist before the report is issued.
No, CD2, Flow Cytometry test home collection is not available. Bone marrow collection is a specialised procedure that must be performed at a diagnostic centre or hospital by a trained professional. The sample also needs to reach the laboratory quickly to preserve cell viability.
If your results show abnormal CD2 expression, your doctor will likely refer you to a haematologist (a specialist in blood disorders) or an immunologist. These specialists can interpret the findings in a full clinical context and advise on any further steps.
Yes, this test is often part of a broader immunophenotyping panel. Doctors may order it together with other CD marker tests to build a complete picture of the cell population. The combination of markers helps classify the type and stage of any suspected blood disorder more precisely.
CD2, Flow Cytometry Test: Booking, Price, and Results
