CD19 Positive B-lymphocyte Count (% and Absolute) Test: Booking, Price, and Results
About CD19 Positive B-lymphocyte Count (% and Absolute) Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD19 B-Cell Count, CD19+ B-Lymphocyte Count, B-Cell Marker CD19, CD19 Pan B-Cell Marker, CD19 Flow Cytometry |
| Sample Type | EDTA blood (venous); bone marrow (sodium heparin and EDTA tubes) |
| Fasting Required | No fasting required |
| Report Time | 3 days |
| Recommended For | All genders and ages; primarily ordered for those with suspected immune disorders, blood cancers, or those receiving B-cell depleting therapies |
| Price | Starting at ₹2,300 |
What is a CD19 Positive B-lymphocyte Count (% and Absolute) Test?
The CD19 Positive B-lymphocyte Count test measures the number and proportion of B-lymphocytes (a type of white blood cell that produces antibodies) in your blood or bone marrow. CD19 is a protein found on the surface of B-cells throughout most stages of their development. The test is also referred to as CD19 B-Cell Count or CD19 Flow Cytometry. Doctors order it when they need to assess how well your immune system is working or to investigate conditions affecting B-cells.
What Does a CD19 Positive B-lymphocyte Count (% and Absolute) Test Measure?
This test uses a technology called flow cytometry, which uses lasers to identify and count specific cells based on their surface markers. It produces two key measurements.
| Parameter | What It Tells You |
|---|---|
| CD19+ B-Cell Percentage | The proportion of B-cells among all lymphocytes in your sample |
| CD19+ B-Cell Absolute Count | The actual number of CD19-positive B-cells per microlitre of blood |
Together, these two values give a full picture of B-cell levels in your immune system.
Why is a CD19 Positive B-lymphocyte Count (% and Absolute) Test Done?
Doctors order this test to investigate a range of immune and blood-related concerns. Below are the most common reasons it is requested.
Common Symptoms That May Require This Test
The following symptoms may prompt your doctor to request a CD19 Positive B-lymphocyte Count test:
- Recurrent or unusual infections that do not resolve easily
- Swollen lymph nodes in the neck, armpits, or groin
- Unexplained weight loss or persistent fatigue
- Night sweats and unexplained fever
- Pale skin or signs of anaemia
- Enlarged spleen or liver detected on examination
Conditions This Test Can Help Detect
This test can assist in identifying or monitoring several conditions:
- B-cell cancers such as chronic lymphocytic leukaemia (CLL), acute lymphoblastic leukaemia (ALL), and lymphomas
- Primary humoral immunodeficiencies, including X-linked agammaglobulinaemia, where B-cells are absent or severely reduced
- Common variable immunodeficiency (CVID), a condition marked by low antibody levels and recurrent lung infections
- Autoimmune conditions such as lupus or rheumatoid arthritis
- B-cell chronic lymphoproliferative disorders, where the test shows high sensitivity in distinguishing cancerous from non-cancerous lymphocyte increases
CD19 Positive B-lymphocyte Count (% and Absolute) Test for Chronic Disease Monitoring
This test plays an important role in tracking patients receiving therapies that target B-cells, such as rituximab, which is used for certain autoimmune diseases, lymphomas, and after organ transplantation. Regular B-cell monitoring during and after such treatment helps identify patients at risk of complications like low antibody levels, poor vaccine response, and susceptibility to infection. Monitoring frequency is typically every 3 to 6 months, depending on the clinical situation.
How to Prepare and What to Expect
No complex preparation is required for this test, but a few simple steps can make the process smoother.
Do You Need to Fast?
No, fasting is not required. You may eat and drink normally before the test. If additional tests are ordered alongside this one, ask your doctor whether those require fasting.
Practical Tips Before Your Test
Keep the following in mind before your appointment:
- Bring a detailed clinical history report, including your symptoms, previous test results, and current medications, as this is required for the test
- Inform your doctor about any immunosuppressive drugs or monoclonal antibody therapy you are receiving
- Stay well hydrated before the test, as drinking water makes vein access easier
- Avoid testing during an active infection where possible, since infections can temporarily alter lymphocyte counts
- Wear loose or half-sleeve clothing to allow easy access to your arm
Step-by-Step Procedure
This test may use blood or bone marrow, depending on your doctor's instructions. Below is what to expect for each sample type.
Blood Sample Collection:
- A tourniquet is placed around your upper arm to make your veins more visible.
- The skin over the puncture site is cleaned with an antiseptic.
- About 3 ml of blood is drawn into a special EDTA tube that prevents clotting.
- The needle is removed, and a small bandage is applied to the site.
- The sample is transported refrigerated and processed in the laboratory using flow cytometry.
Bone Marrow Sample Collection:
- You will be asked to lie still, and the skin over the collection site (usually the hip bone) is cleaned and numbed with local anaesthetic.
- A specialised needle is inserted into the bone to withdraw a small bone marrow sample into both a sodium heparin tube and an EDTA tube.
- The site is dressed after collection, and you may rest briefly before leaving.
- Both samples are stored at 2 to 8 degrees Celsius and dispatched to the laboratory for flow cytometry analysis.
Factors That Can Affect Accuracy
Several factors can influence the reliability of your results:
- Active viral or bacterial infections can temporarily raise lymphocyte counts
- B-cell depleting therapies such as rituximab can keep CD19 counts low for months after treatment
- Immunosuppressive or chemotherapy drugs may reduce B-cell levels
- Samples must be collected and transported correctly; improper storage can affect results
- The timing of sample collection matters, especially for patients being monitored serially over time
Understanding Your CD19 Positive B-lymphocyte Count (% and Absolute) Test Results
Your results will show both a percentage and an absolute count for CD19-positive B-cells. A qualified doctor must review these values in the context of your full medical history.
| Parameter | Normal Range | Unit |
|---|---|---|
| CD19+ B-Cell Percentage | 5 to 31 | % of total lymphocytes |
| CD19+ B-Cell Absolute Count | 100 to 600 | cells per µL |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
A count within the normal range generally suggests your B-cell immune response is functioning as expected. Results above the normal range may point to certain blood cancers or a reactive increase due to infection. Results below the normal range may indicate immune suppression, a primary immunodeficiency, or the effect of B-cell depleting therapy.
Results During Special Conditions
Certain situations can affect how your results are interpreted:
- Recent infections: Viral or bacterial infections can cause a temporary rise in lymphocyte counts that does not reflect an underlying disease.
- Recent immunotherapy: B-cell depleting treatments can suppress CD19 counts for an extended period after the last dose.
- Autoimmune disorders: These can cause unpredictable fluctuations in B-cell numbers over time.
- Age and stress: Natural variation in lymphocyte levels can occur with ageing or prolonged physical or emotional stress.
How to Maintain Healthy Levels
These tips support general immune wellbeing, though they are not a substitute for medical treatment:
- Eat a balanced diet that includes a variety of fruits, vegetables, and lean proteins to help maintain immune function.
- Avoid smoking and limit alcohol, as both can affect the immune system over time.
- Follow your doctor's guidance closely if you are being treated for any underlying condition that affects B-cell levels.
Lupin Diagnostics CD19 Positive B-lymphocyte Count (% and Absolute) Test Price
The CD19 Positive B-lymphocyte Count test is priced starting at ₹2,300 at Lupin Diagnostics. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test. The table below lists approximate prices across major Indian cities.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 2300 |
| CHENNAI | 2300 |
| HYDERABAD | 2300 |
| KOLKATA | 2300 |
| NAVI MUMBAI | 2300 |
| PUNE | 2300 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Booking your CD19 Positive B-lymphocyte Count test online is straightforward:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The CD19 Positive B-lymphocyte Count test measures the percentage and absolute number of CD19-positive B-cells in your blood or bone marrow. B-cells are white blood cells that produce antibodies to help your body fight infections. This test helps doctors evaluate your immune health and investigate suspected blood or immune disorders.
Your doctor may request this test if you have symptoms suggesting an immune disorder, a blood cancer such as leukaemia or lymphoma, or a primary immunodeficiency. It is also used to track how the immune system responds after bone marrow or organ transplantation, or during treatment with therapies that target B-cells.
No, fasting is not required. You can eat and drink as normal before the test. There are no dietary restrictions specific to this test.
A standard blood draw involves a brief prick at the needle site, which most people find only mildly uncomfortable. If a bone marrow sample is also needed, the area is numbed with a local anaesthetic before collection.
The report is typically available within 3 days of sample collection at Lupin Diagnostics.
Yes. Immunosuppressive drugs, chemotherapy, and monoclonal antibody therapies such as rituximab can significantly lower CD19 B-cell counts. Always inform your doctor about all medications and treatments you are currently receiving before the CD19 Positive B-lymphocyte Count test.
An abnormal result does not automatically indicate a serious condition. Many factors can temporarily affect B-cell counts. Consult a haematologist or immunologist who can review your full clinical picture, recommend any additional tests needed, and advise on appropriate next steps.
CD19 Positive B-lymphocyte Count (% and Absolute) Test: Booking, Price, and Results
