CD13 Flow Cytometry Test
About CD13 Flow Cytometry Test
| Field | Value |
|---|---|
| Also Known As | CD13 Leukaemia Cell Marker, CD13 Immunophenotyping, Aminopeptidase N Flow Cytometry, CD13 Myeloid Marker Test |
| Sample Type | Bone marrow aspirate |
| Fasting Required | No |
| Report Time | 3 days |
| Recommended For | All genders and ages; typically ordered for patients with suspected blood cancers or haematological disorders |
| Price | Starting at ₹2,300 |
What is a CD13 Flow Cytometry Test?
The CD13 flow cytometry test detects and measures a protein called CD13 (also known as Aminopeptidase N) on the surface of cells taken from a bone marrow sample. Doctors order this test when they suspect a patient may have a blood cancer or haematological disorder. It is also known as the CD13 Leukaemia Cell Marker test or CD13 Immunophenotyping.
What Does a CD13 Flow Cytometry Test Measure?
The test examines cell samples using specialised fluorescent antibodies and a flow cytometer, a machine that analyses cells individually. Here are the key things it assesses:
| Parameter | What It Tells Us |
|---|---|
| CD13 Expression | Whether abnormal numbers of cells are carrying the CD13 protein on their surface |
| Cell Expression Pattern | Which cell types are expressing CD13 (granulocytes, monocytes, stem cells, or cancer cells) |
| Myeloid Lineage Identification | Whether cells belong to the myeloid line, which produces red blood cells, platelets, and certain white blood cells |
Results are typically reported as a percentage of cells positive for CD13, along with the intensity of expression. Interpretation always requires comparison with other markers and the patient's clinical findings.
Why is a CD13 Flow Cytometry Test Done?
A doctor may order the CD13 flow cytometry test for several reasons, from investigating unexplained symptoms to monitoring an existing condition.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to request this test:
- Frequent or persistent infections
- Unexplained weight loss
- Shortness of breath
- Bone or joint pain and tenderness
- Swollen lymph nodes in the neck, underarm, groin, or stomach
- A full or heavy feeling under the ribs on the left side
- An enlarged spleen or liver
Conditions This Test Can Help Detect
This test can help identify or support the diagnosis of several haematological conditions:
- Acute myeloid leukaemia (AML), where CD13 is expressed in most cases
- Acute promyelocytic leukaemia (APL), a subtype of AML
- Myelodysplastic syndrome (MDS), a condition where blood cells do not mature properly
- B-cell acute lymphoblastic leukaemia (B-ALL), which can show abnormal CD13 expression
- Lymphoplasmacytic lymphoma (LPL) and chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL)
CD13 Flow Cytometry Test for Chronic Disease Monitoring
Doctors may repeat the CD13 flow cytometry test during leukaemia treatment to check how well the treatment is working. It is also used to detect minimal residual disease (MRD), meaning very small numbers of cancer cells that may remain after treatment. The frequency of testing depends on the specific condition and the treatment plan.
How to Prepare and What to Expect
Preparation for the CD13 Flow Cytometry test is straightforward, but there are a few practical steps to follow before your visit.
Do You Need to Fast?
No fasting is required before this test. You can eat and drink as normal on the day of your appointment.
Practical Tips Before Your Test
Keep the following in mind before you arrive at the centre:
- Bring a detailed clinical history, including your symptoms, previous test results, and any relevant medical records, as this is required for the test
- Inform your healthcare provider about all current medications, as some may affect CD13 levels
- Wear comfortable clothing for sample collection
- Consult your doctor beforehand if you have any concerns about your medical history or medications
Step-by-Step Procedure
The CD13 flow cytometry test procedure involves collecting a bone marrow sample. Here is what to expect:
- You will be asked to confirm your identity and hand over your clinical history documentation at the centre.
- A trained healthcare professional will clean the skin over the bone marrow collection site and administer a local anaesthetic to minimise discomfort.
- A small amount of bone marrow aspirate (approximately 3 ml) is collected using a specialised needle, and stored in sodium heparin and EDTA tubes.
- The sample is labelled, kept refrigerated between 2°C and 8°C, and sent promptly to the laboratory for processing.
- In the laboratory, the sample is treated with fluorescent antibodies and passed through a flow cytometer, which analyses each cell individually.
- A specialist reviews the results and prepares the report, which is typically ready within 3 days.
Factors That Can Affect Accuracy
Several factors can influence the reliability of your results:
- Delayed sample processing, which can cause changes in cell markers and lead to inaccurate readings
- The type of anticoagulant used (sodium heparin vs. EDTA), as this can affect some measured parameters
- Certain medications that may alter CD13 expression levels
- Improper sample storage or transport temperature
Understanding Your CD13 Flow Cytometry Test Results
Your results should always be reviewed with your doctor, who will consider them alongside your symptoms, other test findings, and your medical history.
| Parameter | Normal Finding | Elevated Expression | Reduced Expression |
|---|---|---|---|
| CD13 Expression | Absent on lymphocytes, platelets, and red blood cells; present in expected patterns on granulocytes and monocytes | May indicate AML, APL, MDS, or certain lymphoid leukaemias | May suggest impaired immune function or certain blood disorders; also seen in some MDS patients |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
The type of anticoagulant used to preserve the sample can influence certain parameters. Samples collected in EDTA tend to show more changes with delayed processing compared to those collected in heparin. Certain medications may also affect CD13 levels, which is why it is important to tell your doctor about everything you are currently taking.
How to Maintain Healthy Levels
While CD13 levels are largely determined by underlying medical conditions, the following general habits support overall blood and immune health:
- Eat a balanced diet rich in vegetables, whole grains, and lean proteins to support healthy cell production
- Attend regular health check-ups, particularly if you have a family history of blood cancers or haematological disorders
- Report persistent unexplained symptoms such as fatigue, fever, or unintended weight loss to your doctor without delay
Lupin Diagnostics CD13 Flow Cytometry Test Price
The CD13 flow cytometry test cost differs based on location, starting at ₹2,300 at Lupin Diagnostics. This test requires a visit to a Lupin Diagnostics centre; home collection is not available due to the specialised sample handling and prompt processing this test requires.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 2300 |
| CHENNAI | 2300 |
| HYDERABAD | 2300 |
| KOLKATA | 2300 |
| NAVI MUMBAI | 2300 |
| PUNE | 2300 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your CD13 flow cytometry test online at Lupin Diagnostics:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within 3 days.
Frequently Asked Questions
The CD13 flow cytometry test is used to identify and classify blood cancers, particularly those involving myeloid cells. It helps doctors confirm diagnoses such as acute myeloid leukaemia and myelodysplastic syndrome, and is also used to track how well cancer treatment is working.
No fasting or special dietary preparation is required. However, you must bring a detailed clinical history to your appointment, as this is needed to process the test. Let your doctor know about any medications you are currently taking before the test.
No, the CD13 flow cytometry test cannot confirm leukaemia on its own. It is one part of a wider diagnostic process that includes other cell markers, blood tests, and clinical findings. Your doctor will interpret it alongside all available information before reaching a diagnosis.
Results from the CD13 flow cytometry test are typically available within 3 days of sample collection at Lupin Diagnostics. Your report will be sent to you digitally via email or WhatsApp once it is ready.
The procedure may cause some discomfort. A local anaesthetic is used to reduce pain at the collection site. Most patients find any discomfort brief and manageable. Your healthcare provider will keep you informed throughout the process.
The CD13 flow cytometry test requires specialised sample handling and prompt laboratory processing. For accurate results, the sample should ideally be processed within 18 hours of collection. This makes it necessary to collect the sample at a centre rather than at home.
If your results are outside the expected range, speak with your doctor or a haematologist, a specialist in blood disorders. Abnormal findings do not automatically confirm a diagnosis. Further tests are usually needed before any conclusions can be drawn.
CD13 Flow Cytometry Test
