CD10, Flow Cytometry Test: Booking, Price, and Results
About CD10, Flow Cytometry Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | CD10 Flow Cytometry, CALLA (Common Acute Lymphoblastic Leukaemia Antigen), Neprilysin, Neutral Endopeptidase (NEP), CD10 Immunophenotyping |
| Sample Type | Bone Marrow (collected in Sodium Heparin and EDTA tubes) |
| Fasting Required | No |
| Report Time | 3 Days |
| Recommended For | All ages, both genders; primarily ordered when blood cancers such as leukaemia or lymphoma are suspected |
| Price | Starting at ₹2,300 |
What is a CD10, Flow Cytometry Test?
The CD10, flow cytometry test is a specialised laboratory test that detects the presence of the CD10 protein marker on the surface of cells. CD10, also known as CALLA (Common Acute Lymphoblastic Leukaemia Antigen), helps doctors identify and classify certain blood cancers. The test uses a technique called flow cytometry, which analyses large numbers of cells quickly using laser beams and fluorescent dyes. A bone marrow sample is used for this test.
What Does a CD10, Flow Cytometry Test Measure?
This test measures whether CD10 is present on the surface of cells in the bone marrow, and if so, at what intensity and on what percentage of cells. The following explains what this means in practice.
| Marker | What It Tells Us |
|---|---|
| CD10 (CALLA) | A protein found on certain white blood cells; its presence or absence helps classify the type and stage of blood cancer |
| Percentage of CD10-positive cells | Indicates how many cells in the sample carry this marker |
| Expression intensity | Shows how strongly CD10 is expressed, which aids in disease classification |
CD10 is normally found on early B cells, germinal centre B cells, and certain white blood cells. When it appears on abnormal or cancerous cells, it provides critical information about the disease subtype.
Why is a CD10, Flow Cytometry Test Done?
Doctors order the CD10 flow cytometry test when they suspect a blood cancer or need to classify one that has already been identified. The test helps determine the exact type and stage of disease so that appropriate care can be planned.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to request this test.
- Unexplained, persistent fatigue
- Enlarged lymph nodes (swollen glands in the neck, armpits, or groin)
- Recurrent or unusual infections
- Easy bruising or unexplained bleeding
- Unexplained weight loss
- Night sweats
- Bone pain
Conditions This Test Can Help Detect
This test can assist in identifying the following conditions.
- B-cell Acute Lymphoblastic Leukaemia (B-ALL): most cases are CD10 positive, which places them in the "common ALL" category
- Pro-B ALL: an early-stage form that is typically CD10 negative
- Follicular lymphoma: a common adult non-Hodgkin's lymphoma
- Angioimmunoblastic T-cell lymphoma (AITL): CD10 is detected on T cells in the majority of cases
- Burkitt lymphoma
- Certain subtypes of diffuse large B-cell lymphoma (germinal centre type)
CD10, Flow Cytometry Test for Chronic Disease Monitoring
Once a CD10-positive leukaemia or lymphoma has been diagnosed and treated, this test can play an ongoing role. CD10 expression levels remain consistent even when cancer returns, making the test useful for detecting minimal residual disease, meaning very small numbers of remaining cancer cells. Doctors may order this test periodically to track treatment response and check for signs of relapse.
How to Prepare and What to Expect
Preparation for the CD10, flow cytometry test procedure is straightforward, but the bone marrow collection step requires a brief hospital or clinic visit.
Do You Need to Fast?
No fasting is required before this test. You may eat and drink as normal beforehand. Your healthcare team will advise you of any specific instructions based on your individual situation.
Practical Tips Before Your Test
The following steps will help ensure the test goes smoothly.
- Bring a detailed clinical history report, including your symptoms, previous test results, and any relevant medical records, as this is required for the test
- Inform your doctor about all medications you are currently taking
- Wear comfortable clothing with easy access to your arm, and loose clothing over the hip area if bone marrow collection is expected
- Avoid strenuous physical activity on the day of the procedure
- Follow any additional guidance provided by your haematologist or oncologist
Step-by-Step Procedure
The CD10, flow cytometry test procedure involves collecting a bone marrow sample. Here is what to expect.
- You will be asked to lie on your side or stomach, and the skin over your hip bone will be cleaned and numbed with a local anaesthetic.
- A thin, hollow needle is carefully inserted through the skin into the hip bone.
- A small amount of liquid bone marrow (called an aspirate) is withdrawn into the needle. You may feel brief pressure or a short, dull ache during this step.
- The collected sample, approximately 3 ml in each of two collection tubes (one green-capped heparin tube and one lavender-capped EDTA tube), is labelled and sent to the laboratory.
- In the lab, cells are tagged with fluorescent dyes and passed through a flow cytometer, which uses lasers to measure cell characteristics at high speed.
- A computer analyses the data, and a specialist reviews the results before the report is prepared.
Factors That Can Affect Accuracy
The following factors can influence the reliability of results.
- Quality and freshness of the bone marrow sample (ideally processed within 18 to 36 hours of collection)
- Correct use of anticoagulant tubes (EDTA or heparin)
- Proper storage and transport of the sample at the correct temperature
- Cell viability at the time of analysis
- Incomplete clinical history provided at the time of testing
Understanding Your CD10, Flow Cytometry Test Results
Results from this test are reported as positive, negative, or partial, along with the percentage of cells expressing CD10 and the intensity of that expression. These findings are always interpreted alongside other markers and the patient's clinical picture.
| Finding | What It May Indicate |
|---|---|
| CD10 positive (high expression) | Associated with B-ALL (common ALL subtype), follicular lymphoma, Burkitt lymphoma, or germinal centre-type large B-cell lymphoma |
| CD10 negative | May suggest pro-B ALL, chronic lymphocytic leukaemia (CLL), or non-germinal centre lymphoma subtypes |
| CD10 partial or variable | Interpretation depends on the specific cell population and other markers tested alongside |
In healthy adults, CD10 is found on a very small proportion of circulating lymphocytes, roughly 1 to 3%. In normal bone marrow lymphoid precursors, CD10 expression follows predictable patterns that differ from what is seen in disease.
"These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice."
Results During Special Conditions
Certain factors may alter how results are interpreted.
In newborns, CD10 can appear on circulating immature B cells known as haematogones. This is a normal finding and should not be confused with leukaemia. In follicular lymphoma, CD10 may be expressed more weakly in bone marrow samples than in the original lymph node, which can make interpretation more complex. Additionally, CD10-positive cell populations naturally decrease with age, and this age-related variation is taken into account during result interpretation.
How to Maintain Healthy Levels
This test is not used for routine wellness checks. It is a specialised diagnostic tool. The following general guidance applies to anyone being monitored for a blood disorder.
- Attend all follow-up appointments recommended by your haematologist or oncologist
- Report any new or returning symptoms promptly, such as fatigue, swollen glands, or night sweats
- Maintain an open and detailed conversation with your doctor about your overall health, including any changes in how you feel
Lupin Diagnostics CD10, Flow Cytometry Test Price
The CD10, flow cytometry test cost at Lupin Diagnostics starts at ₹2,300. This test requires a visit to a Lupin Diagnostics centre, as home collection is not available due to the specialised nature of bone marrow sample collection and handling.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 2300 |
| CHENNAI | 2300 |
| HYDERABAD | 2300 |
| KOLKATA | 2300 |
| NAVI MUMBAI | 2300 |
| PUNE | 2300 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to get your CD10, flow cytometry test online booking through Lupin Diagnostics.
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
This test is used to identify and classify blood cancers, particularly those arising from B-lymphocytes. It helps doctors determine whether CD10 is expressed on abnormal cells, which in turn clarifies the subtype and developmental stage of conditions such as leukaemia and lymphoma. The results guide treatment planning.
The bone marrow collection involves a needle inserted into the hip bone under local anaesthetic. You may feel brief pressure or a short ache during the aspiration. Most patients find it tolerable, and the procedure is completed quickly. Your care team will ensure you are as comfortable as possible.
At Lupin Diagnostics, the report is delivered within 3 days. The time is needed for careful laboratory processing, fluorescent cell tagging, laser analysis, and specialist review of the findings.
A CD10-positive result on leukaemic cells is commonly associated with the "common ALL" subtype of B-cell Acute Lymphoblastic Leukaemia. In this context, CD10 positivity is generally linked to a more favourable outlook and better response to standard treatment. However, your doctor will explain what this means for your specific situation.
No. This test requires specialised bone marrow collection and must be processed in a flow cytometry laboratory within a strict timeframe. Home collection is not available for the CD10, flow cytometry test. You will need to visit a Lupin Diagnostics centre.
Yes. Providing a detailed clinical history is a requirement for this test. Information about your symptoms, prior diagnoses, previous test results, and any treatments you have received helps the laboratory specialist interpret the flow cytometry findings accurately and in the correct clinical context.
A standard blood test measures counts and levels of blood components. The CD10 flow cytometry test goes further by examining the surface proteins on individual cells using laser-based technology. It is a highly specialised test used specifically when a blood or bone marrow cancer is suspected or needs to be classified.
CD10, Flow Cytometry Test: Booking, Price, and Results
