CCND1/BCL1 Test
About CCND1/BCL1 Test
| Field | Value |
|---|---|
| Also Known As | IGH/CCND1 FISH, t(11;14) FISH, Cyclin D1/IGH Translocation Test, BCL1/IGH Rearrangement Test, Mantle Cell Lymphoma FISH |
| Sample Type | FFPE Tissue Block |
| Fasting Required | No fasting required |
| Report Time | 8 Days |
| Recommended For | Adults of all genders, primarily individuals over 60 years, with suspected blood cancers |
| Price | Starting at ₹7,000 |
What Is a CCND1/BCL1 Test?
The CCND1/BCL1 test is a specialised genetic test that detects a specific chromosomal change linked to certain blood cancers. It looks for a fusion between the CCND1 gene (on chromosome 11) and the IGH gene (on chromosome 14), known as the t(11;14) translocation. Doctors order this test when they suspect conditions such as mantle cell lymphoma or other related blood disorders. The test uses a method called FISH (Fluorescence In Situ Hybridisation), which uses fluorescent probes to highlight chromosomal changes in a tissue sample.
What Does a CCND1/BCL1 Test Measure?
The CCND1/BCL1 FISH test examines tissue at a chromosomal level. The table below summarises what it analyses and why it matters.
| Component | What It Detects | Why It Matters |
|---|---|---|
| CCND1/IGH Gene Fusion | The t(11;14) chromosomal translocation | Key marker for diagnosing mantle cell lymphoma and related blood cancers |
| Cyclin D1 Overexpression | Abnormal activity of the CCND1 gene | CCND1 regulates cell division; overexpression drives uncontrolled cell growth |
| Chromosomal Abnormalities | Disruption of the G1/S cell cycle checkpoint | Indicates abnormal cell division patterns associated with blood cancer |
Why Is a CCND1/BCL1 Test Done?
A doctor may order this CCND1/BCL1 test when a patient shows signs that could point to a blood cancer, particularly mantle cell lymphoma. Below are the common reasons it is requested.
Common Symptoms That May Require This Test
The following symptoms may prompt a doctor to order this test:
- Swollen lymph nodes (glands), particularly in the neck, armpits, or groin
- Persistent fatigue or unexplained weakness
- Unexplained weight loss over a short period
- Night sweats or recurring low-grade fever
- Abdominal pain, bloating, or a feeling of fullness
- Loss of appetite
- Changes in bowel habits
Conditions This Test Can Help Detect
This test helps identify a range of blood-related conditions, including:
- Mantle cell lymphoma (MCL), a rare subtype of B-cell non-Hodgkin lymphoma
- Multiple myeloma (a cancer of plasma cells in the bone marrow)
- B-prolymphocytic leukaemia
- Plasma cell leukaemia
- Chronic lymphocytic leukaemia (in a small subset of cases)
- Splenic lymphoma with villous lymphocytes
CCND1/BCL1 Test for Chronic Disease Monitoring
This test is also used beyond initial diagnosis. In patients already diagnosed with mantle cell lymphoma or multiple myeloma, it helps doctors track disease progression, assess how well a treatment is working, and detect signs of relapse. Repeat testing at diagnosis and at relapse allows the healthcare team to monitor changes in the translocation status over time.
How to Prepare and What to Expect
The CCND1/BCL1 test procedure involves collecting a tissue sample, which is handled by trained healthcare professionals in a clinical setting. Here is what you need to know before your appointment.
Do You Need to Fast?
No fasting is required before this test. You may eat and drink normally on the day of your appointment. Follow any specific instructions provided by your healthcare team regarding the tissue collection procedure.
Practical Tips Before Your Test
Prepare for your appointment by keeping the following points in mind:
- Bring a detailed clinical history report, including your symptoms, previous test results, and any relevant medical records, as this is required for the test
- Inform your doctor about all current medications, particularly blood thinners or any ongoing chemotherapy drugs
- It is preferable to collect the specimen before starting chemotherapy, if possible
- Discuss any history of bleeding disorders with your healthcare provider before the procedure
- Follow all specific instructions from your healthcare team regarding the sample collection
Step-by-Step Procedure
The sample for this test is an FFPE (formalin-fixed, paraffin-embedded) tissue block, collected and prepared by your medical team. Here is what the process typically involves:
- Your doctor identifies the appropriate tissue site for biopsy based on your clinical presentation and imaging results.
- The area is cleaned and local anaesthesia (numbing medicine) is administered to minimise discomfort.
- A tissue sample is collected via biopsy and prepared as an FFPE tissue block for laboratory analysis.
- The prepared block is packaged securely and transported to the laboratory at ambient temperature (18 to 28 degrees Celsius).
- In the laboratory, FISH analysis is performed: fluorescent probes bind to the CCND1 and IGH chromosomal regions to reveal any fusion or translocation.
- A specialist reviews the findings, and the report is prepared, typically within 8 days.
Factors That Can Affect Accuracy
Several factors can influence the reliability of this test result:
- Poor sample quality or inclusion of necrotic (dead) tissue rather than viable tumour tissue
- Prior chemotherapy, which may reduce the number of detectable tumour cells
- A very low percentage of abnormal cells in the sample, which may lead to a missed result
- Improper storage or delayed transport of the tissue block
- Certain genetic alterations that are very small in size may not be detected by standard FISH techniques
Understanding Your CCND1/BCL1 Test Results
Results from the CCND1/BCL1 FISH test are reported as either positive or negative. Your doctor will review these findings alongside your symptoms, medical history, and other test results before drawing any conclusions.
| Result | Interpretation |
|---|---|
| Negative (No Fusion Detected) | The t(11;14) translocation is not present; it helps rule out mantle cell lymphoma, though other blood disorders cannot be excluded |
| Positive (Fusion Detected) | The t(11;14) translocation is present; strongly associated with mantle cell lymphoma, and also seen in multiple myeloma and other leukaemia subtypes |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain situations can affect how results are interpreted:
- Prior chemotherapy may lower the proportion of abnormal cells in the sample, reducing the sensitivity of the test and potentially leading to a negative result even when the translocation is present.
- Some cases involve very small genetic rearrangements that standard FISH techniques may not detect, meaning a negative result does not entirely rule out a t(11;14)-related disorder.
How to Maintain Healthy Levels
While this test does not have "levels" to maintain in the traditional sense, there are steps you can take to support your overall well-being:
- Attend all scheduled follow-up appointments with your haematologist or oncologist as advised
- Report any new or worsening symptoms, such as unexplained weight loss, night sweats, or swollen lymph nodes, to your doctor promptly
- Maintain a balanced diet and engage in physical activity appropriate to your current health status, as guided by your doctor
Lupin Diagnostics CCND1/BCL1 Test Price
The CCND1/BCL1 test cost at Lupin Diagnostics starts at ₹7,000. This test requires a visit to a Lupin Diagnostics centre, as home collection is not available due to the specialised nature of tissue sample collection.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 7000 |
| CHENNAI | 7000 |
| HYDERABAD | 7000 |
| KOLKATA | 7000 |
| NAVI MUMBAI | 7000 |
| PUNE | 7000 |
Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your CCND1/BCL1 test online with Lupin Diagnostics:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection by trained healthcare professionals.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The CCND1/BCL1 test detects t(11;14) chromosomal translocation, which is a key marker for mantle cell lymphoma. It is also used to identify other blood cancers such as multiple myeloma, B-prolymphocytic leukaemia, and plasma cell leukaemia. Doctors use it to diagnose and classify these conditions accurately.
The test requires an FFPE tissue block, which is a processed tissue sample collected via biopsy. The biopsy is performed by a trained doctor in a clinical setting, under local anaesthesia to minimise discomfort. The tissue is then prepared and sent to the laboratory for FISH analysis.
The procedure uses local anaesthesia, so most patients experience only mild discomfort during collection. Your healthcare team will guide you through the process and take steps to keep you as comfortable as possible.
The report is typically ready within 8 days of the laboratory receiving the sample. This turnaround accounts for the time needed to process the FFPE block and carry out detailed FISH analysis.
A positive result means the t(11;14) translocation has been detected in the tissue sample. This is strongly associated with mantle cell lymphoma, but can also be found in other blood cancers. Your doctor will interpret this result alongside your symptoms and other investigations before making a diagnosis.
No. The CCND1/BCL1 test requires specialised tissue collection that must be carried out by trained healthcare professionals at a clinical centre. Home collection is not available for this test.
Not necessarily. A negative result means the t(11;14) translocation was not detected, which helps rule out mantle cell lymphoma in many cases. However, other types of lymphoma or leukaemia not associated with this translocation cannot be excluded. Your doctor may recommend additional tests to reach a complete diagnosis.
CCND1/BCL1 Test
