c-ANCA/PR3, ELISA Test: Booking, Price, and Results
About c-ANCA/PR3, ELISA Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | PR3-ANCA ELISA, c-ANCA (PR3) ELISA, Proteinase 3 Antibodies, Anti-PR3 ELISA, Cytoplasmic ANCA ELISA |
| Sample Type | Serum (venous blood) |
| Fasting Required | No fasting required |
| Report Time | 2 days |
| Recommended For | All ages; male and female |
| Price | Starting at ₹1,600 |
What is a c-ANCA/PR3, ELISA Test?
The c-ANCA/PR3 ELISA is a blood test that detects a specific type of autoantibody, anti-proteinase 3 (PR3), in the blood. These antibodies target proteinase 3, an enzyme found in neutrophils, white blood cells that help the body fight infection. Doctors order this test when they suspect autoimmune conditions affecting blood vessels, particularly a group of disorders known as ANCA-associated vasculitis. A small serum sample collected from a vein in the arm is used for this test.
What Does a c-ANCA/PR3, ELISA Test Measure?
This test uses the ELISA (Enzyme-Linked Immunosorbent Assay) method to detect and measure IgG-class antibodies directed against proteinase 3. A plate coated with the PR3 antigen is exposed to the patient's serum, and any bound antibodies are identified through a colour-change reaction driven by an enzyme. The intensity of the colour indicates the concentration of antibodies present in the blood.
The table below summarises what the test measures:
| Component | What It Detects | Why It Matters |
|---|---|---|
| PR3-ANCA (IgG) | Antibodies against proteinase 3 in neutrophils | Raised levels are associated with autoimmune blood vessel inflammation, especially granulomatosis with polyangiitis (GPA) |
Why is a c-ANCA/PR3, ELISA Test Done?
This test helps doctors identify autoimmune conditions that cause blood vessel inflammation. It is ordered both for initial diagnosis and for tracking treatment in people already diagnosed with vasculitis.
Common Symptoms That May Require This Test
A doctor may recommend the c-ANCA/PR3 ELISA test when a patient presents with the following symptoms:
- Persistent fever without a clear cause
- Unexplained weight loss and ongoing fatigue
- Joint or muscle aches
- Persistent cough or breathlessness
- Blood in the urine or reduced kidney function
- Recurrent nosebleeds or chronic sinus problems
Conditions This Test Can Help Detect
The test can help identify several autoimmune and inflammatory conditions. These include:
- Granulomatosis with polyangiitis (GPA, formerly Wegener's granulomatosis): an autoimmune condition causing inflamed blood vessels in the lungs, kidneys, and sinuses
- Microscopic polyangiitis (MPA): inflammation of very small blood vessels
- Eosinophilic granulomatosis with polyangiitis (EGPA, formerly Churg-Strauss syndrome): an autoimmune condition often linked to asthma
- Inflammatory bowel disease (IBD), particularly ulcerative colitis
- Primary sclerosing cholangitis (PSC), a chronic liver condition
c-ANCA/PR3, ELISA Test for Chronic Disease Monitoring
This test is regularly used to track disease activity and treatment response in patients diagnosed with proteinase 3-related vasculitis. In people with GPA, PR3-ANCA levels often fall with effective treatment, making this test a useful tool for monitoring progress. Doctors typically repeat the test every 3 to 6 months during active treatment, or as clinically needed.
How to Prepare and What to Expect
No special preparation is required for this test. The steps below explain what to expect on the day of sample collection.
Do You Need to Fast?
No fasting is required before the c-ANCA/PR3 ELISA test. You can eat and drink normally before your appointment. If you are booked for other tests on the same day, check with your doctor whether those tests have fasting requirements.
Practical Tips Before Your Test
A few simple steps will help make the process smooth:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test.
- Inform your doctor or the collection team about all current medications, especially immunosuppressants and steroids, as these can affect results.
- Mention any supplements you take, including biotin, as these may interfere with the ELISA method.
- Drink enough water before the appointment so that your veins are easier to locate.
- Bring previous test reports if this is a follow-up for disease monitoring.
Step-by-Step Procedure
Here is what happens during sample collection:
- A trained phlebotomist cleans the skin on the inside of your elbow with an antiseptic wipe.
- A small blood sample of around 3 mL is drawn from a vein in your arm using a fine needle.
- The blood is collected into a yellow-top SST gel tube for serum separation.
- The sample is centrifuged (spun at high speed) so that the serum separates from the blood cells.
- The serum is transferred into a plastic vial and stored at 2 to 8 degrees Celsius before dispatch to the laboratory.
- The laboratory performs the ELISA test on the serum, and your report is ready within 2 days.
Factors That Can Affect Accuracy
Several factors can influence the reliability of your results:
- Use of immunosuppressant or steroid medications before the test
- Concurrent infections at the time of sample collection
- Certain medications, such as hydralazine, which can trigger ANCA antibodies
- Improper sample handling or storage during transport
- Differences in cut-off values across laboratories and assay kits
Understanding Your c-ANCA/PR3, ELISA Test Results
Results are reported as negative or positive based on the antibody concentration measured. Your doctor will review the result alongside your symptoms, medical history, and any other tests ordered.
| Parameter | Negative (Normal) | Positive |
|---|---|---|
| PR3-ANCA (IgG) | Below 5.0 U/mL | 5.0 U/mL or above |
Disclaimer: These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain conditions and medications can affect the result.
- Infections such as endocarditis (infection of the heart valves) and tuberculosis, as well as autoimmune conditions such as lupus, can produce false-positive results.
- The blood pressure medication hydralazine can also trigger ANCA antibodies, leading to a positive reading that does not reflect vasculitis.
- Immunosuppressive therapy can lower antibody levels and may mask active disease in patients already under treatment.
How to Maintain Healthy Levels
These general wellness tips support vascular health and help you stay on top of monitoring:
- Attend all scheduled follow-up appointments so your doctor can track changes in antibody levels over time.
- Report any new or worsening symptoms, such as breathing difficulty, blood in urine, or persistent fatigue, to your doctor without delay.
- Maintain a balanced diet and avoid smoking to support overall vascular and immune health.
Lupin Diagnostics c-ANCA/PR3, ELISA Test Price and Home Collection
The c-ANCA/PR3 ELISA test is available at Lupin Diagnostics starting at ₹1,600, with home sample collection offered across multiple cities in India.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 1800 |
| CHENNAI | 1800 |
| HYDERABAD | 1800 |
| KOLKATA | 2000 |
| NAVI MUMBAI | 1600 |
| PUNE | 1600 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book your c-ANCA/PR3 ELISA test online:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred time slot.
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Home Collection
c-ANCA/PR3 ELISA test home collection is available across cities in India through Lupin Diagnostics. All samples are processed in NABL-accredited laboratories by qualified professionals. Your digital report is delivered directly to you via email or WhatsApp once ready.
Frequently Asked Questions
This test detects IgG antibodies against proteinase 3 (PR3), an enzyme found inside neutrophils. It is primarily used to help diagnose ANCA-associated vasculitis conditions, such as granulomatosis with polyangiitis (GPA), and to monitor disease activity in patients already on treatment.
No fasting is needed. You can eat and drink normally before your sample is collected. Do inform the collection team about any medications or supplements you are currently taking.
Yes. For the c-ANCA/PR3 ELISA test, home collection is available. A trained phlebotomist will visit your home at a time that suits you, collect the blood sample, and send it to the laboratory for testing.
A positive result alone is not sufficient to confirm a diagnosis. It must be interpreted alongside your symptoms, clinical examination, and additional investigations such as biopsy or imaging. Your doctor will make the final assessment.
Infections such as endocarditis and tuberculosis, autoimmune conditions such as lupus, and certain medications (including hydralazine) can lead to a positive result even when vasculitis is not present. Your doctor will consider these possibilities when reviewing your result.
The frequency depends on your doctor's advice and your current treatment plan. In general, the test is repeated every 3 to 6 months during active treatment so that your doctor can track changes in antibody levels and adjust therapy if needed.
c-ANCA/PR3, ELISA Test: Booking, Price, and Results
