BRCA 1 and BRCA2 Germline+ HRR 16 Genes including TP53 Test
About BRCA 1 and BRCA2 Germline+ HRR 16 Genes including TP53 Test
| Field | Value |
|---|---|
| Also Known As | BRCA1/2 + HRR Gene Panel, Hereditary Cancer Panel, Germline HRR Gene Panel with BRCA and TP53, Multi-Gene Cancer Susceptibility Panel |
| Sample Type | Peripheral blood (EDTA tube) or FFPE block |
| Fasting Required | No fasting required |
| Report Time | 25 days |
| Recommended For | Adults of any gender with a personal or family history of breast, ovarian, prostate, or pancreatic cancer; early-onset cancer diagnosis |
| Price | Starting at ₹75,000 |
What is a BRCA 1 and BRCA2 Germline+ HRR 16 Genes including TP53 Test?
The BRCA 1 and BRCA2 Germline+ HRR 16 Genes including TP53 test is a specialised genetic test that looks for inherited mutations in genes involved in repairing damaged DNA. It is prescribed for people with a personal or family history of certain cancers, particularly breast, ovarian, prostate, or pancreatic cancer. A blood sample or preserved tissue block is used for analysis. This test is also known as the BRCA1/2 + HRR Gene Panel or the Germline HRR Gene Panel with BRCA and TP53.
What Does a BRCA1 and BRCA2 Germline+ HRR 16 Genes, including TP53 Test Measure?
The BRCA 1 and BRCA2 Germline+ HRR 16 Genes, including TP53 test, examine multiple genes that work together to repair DNA damage inside cells. When any of these genes carries a harmful change, the repair process breaks down, raising the risk of certain cancers. The panel covers the following gene groups:
| Gene(s) | Role |
|---|---|
| BRCA1 and BRCA2 | Tumour suppressor genes; help slow cell division and repair DNA damage |
| TP53 | Controls cell growth and division; mutations can allow cells to multiply without control |
| ATM, CHEK1, CHEK2, CDK12 | Signal DNA damage and coordinate repair responses |
| PALB2, BARD1, BRIP1 | Support BRCA1/2 function in the DNA repair pathway |
| RAD51B, RAD51C, RAD51D, RAD54L | Key players in homologous recombination repair (HRR), a process that fixes breaks in DNA |
| FANCL | Part of the Fanconi anaemia pathway, which also feeds into HRR |
Why is a BRCA1 and BRCA2 Germline+ HRR 16 Genes including TP53 Test Done?
A doctor may recommend this test for several reasons, depending on a patient's personal health history or their family's cancer history.
Common Symptoms That May Require This Test
The following situations commonly lead a doctor to request this test:
- Personal diagnosis of breast or ovarian cancer, particularly at a young age
- Diagnosis of triple-negative breast cancer before the age of 60
- Male breast cancer at any age
- Two or more separate primary cancers in the same individual
- Close family members with breast, ovarian, prostate, or pancreatic cancer
- A known family member who carries a BRCA or HRR gene mutation
- Early-onset cancer (typically before the age of 45 to 50)
Conditions This Test Can Help Detect
This panel can provide information relevant to the following inherited conditions:
- Hereditary Breast and Ovarian Cancer (HBOC) syndrome, associated with BRCA1 and BRCA2 mutations
- Li-Fraumeni syndrome, caused by TP53 gene mutations, which raises the risk of several cancer types, including breast, bone, and muscle cancers
- Hereditary prostate and pancreatic cancer risk linked to BRCA2 and other HRR gene mutations
- Homologous Recombination Deficiency (HRD) in ovarian cancer, which affects how cancer cells may respond to certain treatments
How to Prepare and What to Expect
No complex preparation is needed for this test, but there are a few steps that will help the process go smoothly.
Do You Need to Fast?
No. Fasting is not required before this test. You can eat and drink normally before giving your sample.
Practical Tips Before Your Test
Keep the following in mind before your appointment:
- Bring a detailed clinical history report, including your symptoms, previous test results, and family history, as this is required for the test
- Carry any previous cancer-related medical records or pathology reports
- Note down your family's cancer history, including the type of cancer, age at diagnosis, and the family member affected
- Consider speaking to a genetic counsellor before the test to understand what the results may mean for you and your family
- Wear a top with easy access to your inner arm for blood collection
Step-by-Step Procedure
The sample collection for the BRCA 1 and BRCA2 Germline+ HRR 16 Genes including TP53 test procedure, follows these steps:
- A trained phlebotomist cleans a small area on your inner arm with an antiseptic.
- A small needle is used to draw approximately 2 ml of blood from a vein. You may feel a brief sting. The process typically takes under 5 minutes.
- The blood is collected into an EDTA (lavender-top) tube and labelled with your details. Alternatively, a preserved tissue block (FFPE block) may be submitted if a tissue sample is being used.
- The sample is stored at 2 to 8 degrees Celsius and sent to the molecular genetics laboratory.
- In the laboratory, DNA is extracted and analysed using Next-Generation Sequencing (NGS), a method that reads thousands of gene segments simultaneously.
- Molecular geneticists review the findings and prepare a detailed report, which is sent to your doctor within 25 days.
Factors That Can Affect Accuracy
The following factors may influence how reliable the results are:
- Poor sample quality due to improper collection or storage
- An incomplete or inaccurate family history provided at the time of testing
- Variants of Uncertain Significance (VUS), where a gene change is found but its clinical meaning is not yet clear
- Laboratory accreditation and the quality of the sequencing platform used
Understanding Your BRCA1 and BRCA2 Germline+ HRR 16 Genes including TP53 Test Results
Results from this test are reported as gene variant classifications, not as numerical values. The table below explains the three possible categories of results.
| Result Type | What It Means |
|---|---|
| Negative (No pathogenic variant detected) | No harmful gene changes found; cancer risk is not raised above the general population level for the genes tested |
| Positive (Pathogenic or Likely Pathogenic variant) | A harmful mutation has been identified; the individual carries an increased risk of developing certain cancers |
| Variant of Uncertain Significance (VUS) | A gene change was found, but its clinical importance has not yet been established |
"These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice."
How to Maintain Healthy Levels
While genetic mutations cannot be changed, general lifestyle habits can support overall well-being:
- Follow a balanced diet and maintain a healthy weight through regular physical activity
- Avoid tobacco and limit alcohol intake, as these are known to affect cancer risk independently
- Attend all recommended screening appointments and follow-up consultations as advised by your doctor
Lupin Diagnostics BRCA 1 and BRCA2 Germline+ HRR 16 Genes including TP53 Test Price
The BRCA1 and BRCA2 Germline+ HRR 16 Genes including TP53 test price starts at ₹75,000 at Lupin Diagnostics. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 75000 |
| CHENNAI | 75000 |
| HYDERABAD | 75000 |
| KOLKATA | 75000 |
| NAVI MUMBAI | 75000 |
| PUNE | 75000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book the BRCA1 and BRCA2 Germline+ HRR 16 Genes including TP53 test online booking:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within 25 days.
Frequently Asked Questions
This test is recommended for people with a personal or family history of breast, ovarian, prostate, or pancreatic cancer. It is also suggested for those diagnosed with breast cancer before the age of 45, those with triple-negative breast cancer, or anyone with a close relative known to carry a BRCA or HRR gene mutation. Your doctor will advise whether this test is appropriate for your situation.
Germline testing, like the BRCA1 and BRCA2 Germline+ HRR 16 Genes including TP53 test, looks for inherited mutations present in every cell of the body from birth. Somatic testing, by contrast, looks for mutations that develop only within tumour tissue and are not passed on to children. They serve different clinical purposes and are not interchangeable.
Results for this panel are typically delivered within 25 days from the time the sample is received at the laboratory. This extended turnaround is because the test analyses 16 or more genes using Next-Generation Sequencing, which requires detailed review by molecular geneticists.
Yes. Men can carry BRCA1, BRCA2, and other HRR gene mutations. Male carriers of these mutations face an increased risk of prostate cancer and, in some cases, male breast cancer. The test is relevant for men with a strong family history of hereditary cancers or those with a confirmed family mutation.
TP53 is the second most commonly mutated gene in early-onset breast cancer. Changes in this gene are linked to Li-Fraumeni syndrome, a condition that significantly raises lifetime cancer risk across several cancer types. Including TP53 in the panel ensures that this important risk factor is not missed, particularly in younger patients.
A VUS means a gene change was detected, but scientists do not yet have enough evidence to confirm whether it is harmful or harmless. It is not a positive result. Over time, as more data becomes available, a VUS may be reclassified. Testing other family members can sometimes help clarify the significance of a VUS. Your genetic counsellor can explain the implications in detail.
Genetic counselling is strongly advised before deciding to take this test and again when receiving the results. A genetic counsellor helps you understand what the test can and cannot tell you, what a positive or negative result means for you and your family, and what steps may follow. This support is particularly important because the results can have implications beyond just the individual being tested.
BRCA 1 and BRCA2 Germline+ HRR 16 Genes including TP53 Test
