BRCA 1 & 2 Germline with TP53 Gene Panel Test
About BRCA 1 & 2 Germline with TP53 Gene Panel Test
| Field | Value |
|---|---|
| Also Known As | BRCA1/BRCA2/TP53 Gene Panel, Hereditary Breast and Ovarian Cancer Panel with TP53, HBOC/LFS Panel, Breast Cancer Gene Panel |
| Sample Type | Peripheral blood (EDTA tube) |
| Fasting Required | No fasting required |
| Report Time | 16 days |
| Recommended For | Adults of any gender with a personal or family history of breast, ovarian, pancreatic, or prostate cancer; individuals meeting hereditary cancer testing criteria |
| Price | Starting at ₹25,000 |
What is a BRCA 1 & 2 Germline with TP53 Gene Panel Test?
The BRCA 1 & 2 Germline with TP53 Gene Panel Test is a genetic blood test that looks for inherited harmful changes (mutations) in three key genes: BRCA1, BRCA2, and TP53. These genes normally help the body suppress tumour growth and repair damaged DNA. When they carry harmful mutations, the risk of developing certain cancers increases significantly.
This test is typically prescribed by an oncologist or a genetic counsellor for people with a personal or family history suggesting a hereditary cancer condition. It is also known as the BRCA1/BRCA2/TP53 Gene Panel or the Hereditary Breast and Ovarian Cancer Panel with TP53.
What Does a BRCA 1 & 2 Germline with TP53 Gene Panel Test Measure?
This panel examines three specific genes in your DNA. Each gene has a distinct role in cancer risk. The table below summarises what each one does and why it matters.
| Gene | What It Does | Why It Is Tested |
|---|---|---|
| BRCA1 | A tumour suppressor gene that helps prevent cancer | Inherited harmful changes are linked to hereditary breast and ovarian cancer; also relevant for treatment selection |
| BRCA2 | Tumour suppressor gene associated with hereditary breast and ovarian cancer syndrome | Variants account for a large proportion of hereditary breast and ovarian cancer families |
| TP53 | Helps stop tumours from growing and repairs damaged DNA within cells | Germline mutations in this gene cause Li-Fraumeni syndrome, a rare inherited cancer predisposition condition |
Why is a BRCA 1 & 2 Germline with TP53 Gene Panel Test Done?
Doctors recommend the BRCA 1 & 2 Germline with TP53 Gene Panel Test when a person's personal or family history suggests an inherited cancer risk. Below are the common reasons this test is ordered.
Common Symptoms That May Require This Test
The following personal or family history features often prompt a referral for the BRCA 1 & 2 Germline with TP53 Gene Panel Test:
- Personal history of breast, ovarian, prostate, or pancreatic cancer diagnosed at a young age
- Family history of multiple cancers across generations
- A known BRCA1, BRCA2, or TP53 mutation already identified in a close relative
- Diagnosis of triple-negative breast cancer (a specific subtype)
- Male breast cancer, which is uncommon and often hereditary
- Bilateral breast cancer (cancer in both breasts)
- Multiple primary cancers occurring in the same person
Conditions This Test Can Help Detect
This test can help identify the genetic basis for the following inherited conditions:
- Hereditary breast and ovarian cancer (HBOC) syndrome, which raises the risk for early-onset breast and ovarian cancer, as well as prostate and pancreatic cancers
- Li-Fraumeni syndrome (LFS), a rare autosomal dominant condition caused by TP53 mutations, is associated with a very high lifetime cancer risk
- Associated cancers include bone and soft-tissue sarcomas, brain tumours, adrenocortical carcinoma, and leukaemia
How to Prepare and What to Expect
The BRCA 1 & 2 Germline with TP53 Gene Panel Test procedure is straightforward. No special physical preparation is needed, but some steps before the appointment will help ensure the process goes smoothly.
Do You Need to Fast?
No. Fasting is not required for this test. You can eat and drink normally before your appointment.
Practical Tips Before Your Test
Here are a few things to do before attending your appointment:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Gather information about the types of cancer, ages at diagnosis, and which relatives were affected in your family
- Meet with a genetic counsellor beforehand to discuss whether this test is right for you and to understand what the results may mean
- Complete any informed consent forms for genetic testing before your sample is collected
- Be prepared that results may carry emotional or familial implications; support from a counsellor can be helpful
Step-by-Step Procedure
The following steps describe what happens during the BRCA 1 & 2 Germline with TP53 Gene Panel Test procedure:
- A healthcare professional cleans the skin on your inner arm and draws a small blood sample from a vein using a standard technique.
- The blood is collected into a lavender-top EDTA tube (a collection tube containing a preservative to keep the sample stable).
- The sample is labelled carefully and stored at a refrigerated temperature of 2 to 8 degrees Celsius before being dispatched to the laboratory.
- In the laboratory, DNA (your genetic material) is extracted from white blood cells in the sample.
- Next-generation sequencing (NGS), an advanced DNA reading technology, is used to examine the BRCA1, BRCA2, and TP53 genes for harmful changes, small DNA variations, and missing or extra copies of gene sections.
- A specialist interprets the sequencing results and generates a report, which is typically ready within 16 days.
Factors That Can Affect Accuracy
The following factors may affect the reliability of your results:
- A previous allogeneic bone marrow transplant (donor transplant) can interfere with germline testing, as donor DNA may be present in the blood sample
- Poor sample quality, insufficient DNA quantity, or improper sample labelling
- Mosaicism (having different genetic makeup in different cells), below the laboratory detection threshold, may not be identified
- Sample contamination during collection or transport
Understanding Your BRCA 1 & 2 Germline with TP53 Gene Panel Test Results
Results from this test are qualitative, meaning they are reported as positive, negative, or as a variant of uncertain significance rather than as a numerical value. Your doctor or genetic counsellor will explain what your specific result means for you and your family.
| Parameter | Result | Interpretation |
|---|---|---|
| BRCA1, BRCA2, TP53 | No pathogenic or likely pathogenic variant detected | Negative — no increased hereditary cancer risk identified from these genes |
| BRCA1, BRCA2, TP53 | Pathogenic or likely pathogenic variant detected | Positive — increased hereditary cancer risk identified; genetic counselling is advised |
| BRCA1, BRCA2, TP53 | Variant of uncertain significance (VUS) detected | Uncertain — a gene change has been found whose impact is not yet fully understood; further evaluation may be recommended |
"These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice."
Results During Special Conditions
Certain situations can affect how results are interpreted:
- A prior allogeneic bone marrow transplant may cause the blood sample to contain donor DNA, which can lead to misleading results. Inform your doctor if you have had this procedure.
- Sample degradation or insufficient DNA from the collected blood may require a repeat collection before a result can be issued.
How to Maintain Healthy Levels
These are general wellness steps relevant after receiving your results:
- Follow a balanced diet and maintain regular physical activity to support overall health
- Attend all recommended cancer screening appointments, such as mammography or MRI, as advised by your doctor
- Discuss personalised cancer surveillance options with a genetic counsellor and oncologist, particularly if your result is positive
Lupin Diagnostics BRCA 1 & 2 Germline with TP53 Gene Panel Test Price
The BRCA 1 & 2 Germline with TP53 Gene Panel Test cost at Lupin Diagnostics starts at ₹25,000. This test requires a visit to a Lupin Diagnostics centre; home collection is not available for this test.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 25000 |
| CHENNAI | 25000 |
| HYDERABAD | 25000 |
| KOLKATA | 25000 |
| NAVI MUMBAI | 25000 |
| PUNE | 25000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
You can get a BRCA 1 & 2 Germline with TP53 Gene Panel Test online booking in a few simple steps:
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time for sample collection.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
People with a personal or family history suggesting an inherited cancer condition should speak to their doctor or a genetic counsellor. This includes those diagnosed with cancer at a young age, those with multiple family members affected by cancer, or those with a known BRCA1, BRCA2, or TP53 mutation in the family.
Germline testing, like this panel, looks for inherited gene changes present in all cells from birth. Somatic testing, by contrast, examines mutations that developed within cancer cells during a person's lifetime. The two tests serve different clinical purposes and are not interchangeable.
A positive result means a harmful mutation has been identified in one of the three genes tested. For BRCA1 or BRCA2, this can mean a significantly elevated lifetime risk of breast or ovarian cancer compared to the general population. A positive TP53 result may indicate Li-Fraumeni syndrome. A positive result does not mean cancer will definitely develop.
Li-Fraumeni syndrome is a rare inherited condition caused by a harmful change in the TP53 gene. It significantly raises the risk of developing several types of cancer, including breast cancer, bone and soft-tissue sarcomas, brain tumours, and others. Most people with this mutation will develop cancer during their lifetime, and genetic counselling is strongly recommended.
Results from the BRCA 1 & 2 Germline with TP53 Gene Panel Test are typically available within 16 days, as this test uses next-generation sequencing technology, which requires detailed laboratory analysis.
Yes. Men who carry a harmful BRCA2 mutation face an elevated lifetime risk of breast cancer and a three to seven times higher risk of prostate cancer compared to the general population. BRCA2-associated prostate cancers also tend to be more aggressive. Testing is relevant for men with a significant family history of these cancers.
Genetic counselling is strongly advised before and after taking this test. A genetic counsellor helps you understand what the test involves, what the results may mean for you and your family, and what steps to consider based on your findings. This support is especially important given the sensitive nature of hereditary cancer risk information.
BRCA 1 & 2 Germline with TP53 Gene Panel Test
