1p & 19q Co-deletion by FISH Test: Booking, Price, and Results
About 1p & 19q Co-deletion by FISH Test: Booking, Price, and Results
| Field | Value |
|---|---|
| Also Known As | 1p/19q Co-deletion FISH, 1p19q deletion by FISH, Oligodendroglioma FISH panel, Chromosomes 1p and 19q Co-deletion test |
| Sample Type | Fresh tumour tissue, formalin-fixed paraffin-embedded (FFPE) tissue block/slides, biopsy tissue |
| Fasting Required | No |
| Report Time | 9 Days |
| Recommended For | Adults and children with suspected brain gliomas, regardless of gender |
| Price | Starting at ₹10,000 |
What is a 1p & 19q Co-deletion by FISH Test?
The 1p & 19q co-deletion by FISH test is a specialised molecular analysis performed on brain tumour tissue. It uses a technique called fluorescence in situ hybridisation (FISH), which employs fluorescent probes to detect the loss of specific chromosomal segments within tumour cells. The test looks for simultaneous deletion of the short arm of chromosome 1 (1p) and the long arm of chromosome 19 (19q). It is also known as the 1p/19q co-deletion FISH test or Oligodendroglioma FISH panel, and is typically requested by oncologists or neurosurgeons following a brain tumour biopsy.
What Does a 1p & 19q Co-deletion by FISH Test Measure?
This test analyses genetic material from tumour tissue to identify specific chromosomal deletions. The key findings are listed below.
| Parameter | What it Represents |
|---|---|
| 1p deletion | Loss of genetic material on the short arm of chromosome 1 |
| 19q deletion | Loss of genetic material on the long arm of chromosome 19 |
| 1p/19q co-deletion | Simultaneous loss of both chromosomal arms; a key diagnostic marker for oligodendroglioma |
| Signal ratio | Numerical ratio used to confirm whether a deletion is present or absent |
Why is a 1p & 19q Co-deletion by FISH Test Done?
This test is ordered when a brain tumour is identified, and the treating doctor needs molecular information to classify it accurately. It guides diagnosis, prognosis, and treatment planning.
Common Symptoms That May Require This Test
A doctor may request the 1p & 19q co-deletion by FISH test when a patient presents with the following symptoms, particularly in the setting of a known brain tumour.
- Seizures (one of the most frequently reported symptoms in glioma patients)
- Persistent or worsening headaches
- Focal neurological deficits such as limb weakness or numbness
- Speech and language difficulties
- Memory problems or cognitive changes
- Personality or behavioural changes
Conditions This Test Can Help Detect
The test helps identify or differentiate the following conditions.
- Oligodendroglioma, defined by IDH mutation together with 1p/19q co-deletion
- Astrocytoma and other diffuse gliomas (by exclusion, when co-deletion is absent)
- Low-grade and high-grade oligodendroglial tumours requiring specific treatment planning
- Gliomas where chemosensitivity and radiation response prediction are needed
How to Prepare and What to Expect
The 1p & 19q co-deletion by FISH test procedure does not require any preparation from the patient before the biopsy. The steps below explain how the test is carried out.
Do You Need to Fast?
No fasting is required for this test. The analysis is performed on tumour tissue, not a blood or urine sample.
Practical Tips Before Your Test
The following points will help ensure the process goes smoothly.
- Bring a detailed clinical history, including symptoms, previous imaging reports, pathology results, and family history, as this is required for the test
- Follow all instructions from your neurosurgeon or oncologist regarding the biopsy procedure
- Ensure that the tissue block submitted contains tumour tissue, as confirmed by your surgical team
- Inform your doctor about all current medications before the biopsy
Step-by-Step Procedure
Here is what happens during sample collection and laboratory processing for the 1p & 19q co-deletion by FISH test.
- A neurosurgeon performs a brain biopsy, typically by inserting a needle through a small opening in the skull to obtain a sample of tumour tissue.
- The tissue is fixed in formalin and embedded in paraffin wax (FFPE), which preserves it for laboratory analysis.
- In the laboratory, the paraffin is removed from the tissue sections, and the tissue is treated with an enzyme (pepsin) to allow the fluorescent probes to access the DNA.
- The DNA is heated to separate its strands, then fluorescent probe sets targeting the 1p, 1q, 19p, and 19q chromosomal regions are applied and allowed to bind.
- Excess unbound probes are washed away, and the cell nuclei are stained so they can be visualised clearly.
- A trained specialist examines the tissue under a fluorescence microscope, counting the fluorescent signals in each cell to determine whether a deletion is present.
Factors That Can Affect Accuracy
The following factors may affect the reliability of the test result.
- Use of fixatives other than formalin during tissue processing, which can make results uninterpretable
- Decalcified tissue specimens, which are generally not suitable for FISH analysis
- Insufficient tumour cell content in the submitted tissue block
- Improper fixation or embedding during surgical processing
- The level of experience of the laboratory personnel performing the analysis
Understanding Your 1p & 19q Co-deletion by FISH Test Results
Results from this test are reported qualitatively, not as numerical values. Your oncologist will interpret the findings alongside imaging reports, pathology results, and other molecular markers such as IDH mutation status.
| Finding | Interpretation |
|---|---|
| 1p/19q co-deletion detected (Positive) | Supports a diagnosis of oligodendroglioma; associated with a favourable prognosis and better response to treatment |
| 1p/19q co-deletion not detected (Negative) | Does not support oligodendroglioma; the tumour may be an astrocytoma or another glioma subtype |
| Isolated 1p deletion detected | Requires correlation with other molecular markers |
| Isolated 19q deletion detected | Isolated 19q loss is more commonly seen in astrocytomas |
These ranges are general guidelines. Your doctor will interpret your results based on your age, health history, and other factors. Always consult a qualified healthcare professional for personalised medical advice.
How to Maintain Healthy Levels
Since this is a genetic test performed on tumour tissue, "normal levels" do not apply in the conventional sense. The following tips support overall well-being during and after the diagnostic process.
- Follow all follow-up appointments, monitoring scans, and treatment recommendations given by your healthcare team
- Maintain a balanced diet, adequate hydration, and regular rest to support recovery after a biopsy procedure
- Reach out to your treating team with any new or worsening symptoms rather than waiting for a scheduled visit
Lupin Diagnostics 1p & 19q Co-deletion by FISH Test Price
The 1p & 19q co-deletion by the FISH test at Lupin Diagnostics costs ₹10,000. This test requires surgically obtained tumour tissue and must be processed in a specialised laboratory; home collection is not available. Please visit your nearest Lupin Diagnostics centre to submit your sample.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 10000 |
| CHENNAI | 10000 |
| HYDERABAD | 10000 |
| KOLKATA | 10000 |
| NAVI MUMBAI | 10000 |
| PUNE | 10000 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Follow these steps to book the 1p & 19q co-deletion by FISH test online or in person.
- Select the test on the Lupin Diagnostics website.
- Choose your city and preferred centre location.
- Visit the centre at your scheduled time to submit the FFPE tissue block or biopsy sample.
- Receive your report via email or WhatsApp within the stipulated turnaround time.
Frequently Asked Questions
The 1p & 19q co-deletion by FISH test is a specialised molecular technique that uses fluorescent probes on brain tumour tissue to detect the simultaneous loss of two chromosomal segments: the short arm of chromosome 1 (1p) and the long arm of chromosome 19 (19q). This co-deletion is a defining molecular marker for a specific type of brain tumour called oligodendroglioma. Results help guide diagnosis and treatment planning.
The 1p/19q co-deletion, together with an IDH gene mutation, is required to confirm a diagnosis of oligodendroglioma under current classification guidelines. A positive finding is also associated with a better response to chemotherapy and radiation therapy, as well as improved long-term survival. This makes the test a key step in deciding the most appropriate treatment plan.
The sample is tumour tissue obtained during a brain biopsy, which is a surgical procedure performed by a neurosurgeon. The tissue is then fixed in formalin and embedded in paraffin to preserve it before being sent to the laboratory for FISH analysis. Patients do not need to provide any additional samples, such as blood or urine.
No. This test requires surgically collected brain tumour tissue that must be processed in a specialised laboratory. Home collection is not available. The tissue block or slides must be submitted to a Lupin Diagnostics centre.
A positive result means that both the 1p and 19q chromosomal deletions were detected in the tumour cells. This finding supports a diagnosis of oligodendroglioma and indicates a more favourable prognosis. It also suggests that the tumour may respond well to specific chemotherapy and radiation regimens. Your oncologist will explain what this means for your individual treatment plan.
The 1p & 19q co-deletion by FISH test report is typically ready in 9 days after the laboratory receives the tissue sample. This turnaround time accounts for the multi-step laboratory processing involved in FISH analysis.
Yes. Clinical history is required when submitting the sample for this test. You should provide relevant details, including the patient's symptoms, previous imaging findings, surgical notes, and any earlier pathology reports. This information helps the laboratory team process and interpret the results accurately.
1p & 19q Co-deletion by FISH Test: Booking, Price, and Results
