Prothrombin (Factor II) Mutation Test
About Prothrombin (Factor II) Mutation Test
| Field | Value |
|---|---|
| Also Known As | Prothrombin G20210A Mutation Test, Factor II Mutation Test, F2 Gene Mutation Test, PT G20210A Test |
| Sample Type | Whole blood (EDTA tube) |
| Fasting Required | No fasting required |
| Report Time | 5 days |
| Recommended For | Adults and children of all genders; particularly those with a personal or family history of blood clots |
| Price | Starting at ₹3,700 |
What is a Prothrombin (Factor II) Mutation Test?
The Prothrombin (Factor II) Mutation test is a genetic test that checks for a specific change in the F2 gene. This gene carries the instructions for making prothrombin, a protein that plays a key role in blood clotting. The test detects a known mutation called G20210A, which can cause the body to produce too much prothrombin, raising the risk of abnormal blood clots.
Also called the Factor II Mutation test or the Prothrombin G20210A Mutation test, it is performed on a small whole blood sample. Doctors order this test when a patient has had unexplained blood clots or has a family history of clotting disorders.
What Does a Prothrombin (Factor II) Mutation Test Measure?
This test analyses the F2 gene in your DNA to detect the G20210A variant. Here is what each component involves:
- F2 Gene: The gene responsible for producing prothrombin, a protein essential to the normal clotting process.
- Prothrombin (Factor II): A protein that circulates in the blood in an inactive form. When injury occurs, it converts into thrombin, which then helps form a blood clot.
- G20210A Variant: A specific change in the DNA code of the F2 gene. When present, it leads to excess prothrombin in the bloodstream, increasing clot risk.
- Genotype: The test reports whether the mutation is absent, present in one copy (heterozygous), or present in both copies (homozygous) of the gene.
Why is a Prothrombin (Factor II) Mutation Test Done?
A doctor may order the Prothrombin (Factor II) Mutation test when a patient shows signs of a clotting problem or has a family history of inherited clotting disorders. The test helps identify a genetic cause for unexplained blood clots.
Common Symptoms That May Require This Test
Certain symptoms may prompt your doctor to recommend this test. These include:
- Leg pain, tenderness, or swelling (possible signs of deep vein thrombosis)
- Skin that appears purple or red and feels warm to the touch
- Chest pain without a clear cause
- Shortness of breath
- A very fast heartbeat
- Recurring unexplained blood clots
- Blood clots occurring at a young age in an otherwise healthy person
Conditions This Test Can Help Detect
This test can help identify or confirm the following conditions:
- Prothrombin thrombophilia, an inherited condition that raises the risk of venous blood clots
- Deep vein thrombosis (DVT), where clots form in deep veins, usually in the legs
- Pulmonary embolism (PE), where a blood clot travels to the lungs
- Venous thromboembolism (VTE), a broader term covering clots in the veins
How to Prepare and What to Expect
No special preparation is needed for the Prothrombin (Factor II) Mutation test procedure. However, there are a few things to be aware of before your appointment.
Do You Need to Fast?
No. Fasting is not required before this test. You may eat and drink normally on the day of your appointment.
Practical Tips Before Your Test
Keep these points in mind before heading to your sample collection:
- Bring a detailed clinical history, including your symptoms, previous test results, and family history, as this is required for the test
- Tell your doctor about all medications you are currently taking, as certain drugs may affect test results
- Inform your doctor if you have recently received a blood transfusion, as this may interfere with the results
- Let your doctor know if you have had a bone marrow or stem cell transplant, as this can affect DNA-based testing
- Wear a short-sleeved top or clothing with sleeves that can be rolled up easily
- Stay well hydrated, as this helps with blood collection
Step-by-Step Procedure
The Prothrombin (Factor II) Mutation test procedure involves a simple blood draw. Here is what to expect:
- A trained phlebotomist will clean the skin on your arm, usually at the inner elbow, with an antiseptic
- A small amount of blood, about 2 ml, is drawn from a vein using a fine needle
- The blood is collected into a lavender-top EDTA tube, which prevents the sample from clotting during transport
- The sample is labelled and stored at the correct temperature before being sent to the laboratory
- In the lab, DNA is extracted from the blood cells and the F2 gene is examined using Real Time PCR (polymerase chain reaction), a highly accurate method for detecting specific genetic changes
- The results are reviewed and dispatched within the turnaround time
Factors That Can Affect Accuracy
Certain factors may affect the reliability of your results:
- Recent blood transfusions, which can introduce donor DNA into the sample
- A previous bone marrow or stem cell transplant from a donor with a different genotype
- Improper sample handling or storage during transport
- Rare genetic variations near the test site that may affect how the PCR assay performs
Understanding Your Prothrombin (Factor II) Mutation Test Results
Your results will indicate whether the G20210A mutation is present in your F2 gene. Because this is a genetic test, it reports a genotype rather than a number. The table below explains the possible outcomes.
| Result | Interpretation |
|---|---|
| Not Detected (Wild Type) | No mutation found; normal clotting risk from this gene |
| Heterozygous (one copy) | One copy of the mutated gene; moderately increased clotting risk (two to five times higher than average) |
| Homozygous (two copies) | Both copies of the gene are mutated; significantly increased clotting risk |
Disclaimer: This information is a general guide. Your doctor will review your genetic results alongside your age, health history, and other clinical findings. Always consult a qualified healthcare professional for personalised medical advice.
Results During Special Conditions
Certain situations can affect how results are interpreted:
- Carrying both the Prothrombin G20210A mutation and the Factor V Leiden mutation together raises the clotting risk approximately 15 times compared to the general population.
- Women who carry one copy of this mutation and take combined oral contraceptive pills have roughly a 15-fold increased risk of venous thromboembolism.
- Pregnancy on its own raises clotting risk; the presence of this mutation alongside pregnancy may further elevate that risk.
Managing Your Clotting Risk
Since this is a genetic result, it cannot be changed. However, you can manage your overall clotting risk with these general wellness steps:
- Stay physically active and avoid sitting or lying down for long, uninterrupted periods
- Maintain a healthy body weight to reduce pressure on your veins
- Speak with your doctor before starting oral contraceptives or hormone replacement therapy, as these may raise clotting risk if the mutation is present
Lupin Diagnostics Prothrombin (Factor II) Mutation Test Price and Home Collection
The Prothrombin (Factor II) Mutation test is available at Lupin Diagnostics starting at ₹3,700, with home collection available for your convenience.
| City | Approximate Price (₹) |
|---|---|
| BHOPAL | 3700 |
| CHENNAI | 3700 |
| HYDERABAD | 3700 |
| KOLKATA | 3700 |
| NAVI MUMBAI | 3700 |
| PUNE | 3700 |
Disclaimer: Prices are indicative and may vary by location. Please confirm the current price at the time of booking.
How to Book
Booking the Prothrombin (Factor II) Mutation test online is straightforward:
- Select the test on the Lupin Diagnostics website
- Choose your city and preferred time slot
- Opt for home sample collection by a certified phlebotomist, or visit your nearest Lupin Diagnostics centre
- Receive your report via email or WhatsApp within the stipulated turnaround time
Home Collection
Lupin Diagnostics offers home collection for this test across multiple cities in India. All samples are processed in NABL-accredited laboratories by trained professionals. Once your results are ready, you can access your digital report via email or WhatsApp.
Frequently Asked Questions
The Prothrombin (Factor II) Mutation test is a genetic test that checks for a specific change in the F2 gene, known as the G20210A variant. This mutation causes the body to produce excess prothrombin, a clotting protein, which increases the risk of developing blood clots in the veins.
Your doctor may recommend this test if you have experienced unexplained blood clots on two or more occasions, developed a blood clot at a young age, or have a close family member with a known clotting disorder. It is a one-time genetic test because the result does not change over time.
No fasting is required before this test. You can eat, drink, and continue taking your usual medications unless your doctor advises otherwise.
No. This test analyses your DNA, which remains unchanged from birth. Diet, exercise, and lifestyle choices do not affect the genetic result. However, lifestyle factors may influence your overall risk of developing blood clots.
No. Many people who carry the Prothrombin G20210A mutation never develop a blood clot. A positive result indicates an increased risk but does not guarantee that a clot will occur. Your doctor will interpret the result along with your personal and family medical history.
Yes. If you carry one copy of the mutation (heterozygous), each child has a 50% chance of inheriting it. If you carry two copies (homozygous), all children will inherit at least one copy of the mutation. A genetic counsellor can help explain the implications for your family.
At Lupin Diagnostics, results are typically available within 5 days from the date of sample collection. Your digital report will be shared via email or WhatsApp once it is ready.
Prothrombin (Factor II) Mutation Test
